CAF1 (CHAF1A) (NM_005483) Human Tagged ORF Clone

CAT#: RC209138

CHAF1A (Myc-DDK-tagged)-Human chromatin assembly factor 1, subunit A (p150) (CHAF1A)

Product Images

Western blot validation of overexpression lysate (Cat# LY417270) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209138 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CHAF1A protein (Cat# TP309138). The protein was produced from HEK293T cells transfected with CHAF1A cDNA clone (Cat# RC209138) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHAF1A
• Synonyms: CAF-1; CAF1; CAF1B; CAF1P150; P150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209138 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAGGAGCTGGAGTGCGGGGCGCCCGGCGCCAGGGGAGCCGCCACAGCCATGGATTGCAAAGATA
  GACCAGCTTTTCCAGTTAAGAAGTTAATACAAGCCCGTCTGCCGTTTAAGCGCCTGAATCTTGTCCCAAA
  GGGGAAAGCCGATGACATGTCAGACGATCAGGGTACTTCTGTGCAAAGTAAAAGCCCCGATTTAGAGGCC
  TCTTTGGACACCTTGGAAAACAACTGTCATGTGGGTTCTGACATAGACTTTAGACCGAAACTTGTCAACG
  GGAAGGGTCCCTTAGATAACTTTTTAAGAAATAGAATCGAAACCAGTATTGGCCAGAGCACAGTCATCAT
  TGATTTGACAGAGGACTCGAATGAGCAGCCAGACAGTCTTGTGGACCACAATAAACTAAATTCTGAAGCC
  TCTCCCTCCAGGGAGGCAATAAATGGCCAGCGAGAAGACACTGGGGATCAGCAGGGGTTGTTGAAGGCCA
  TTCAGAACGACAAGTTGGCATTTCCTGGAGAGACCCTTTCAGACATTCCTTGCAAAACAGAGGAGGAGGG
  TGTTGGCTGTGGAGGTGCAGGGAGGAGAGGCGACTCCCAGGAATGTTCGCCACGGAGCTGCCCGGAGCTG
  ACGAGTGGCCCGAGAATGTGCCCCAGAAAGGAGCAGGACAGTTGGAGTGAAGCTGGGGGCATCCTGTTCA
  AAGGGAAGGTGCCTATGGTGGTCTTGCAGGACATCTTGGCTGTGAGACCACCGCAAATCAAGTCCCTTCC
  AGCCACACCCCAAGGCAAGAACATGACCCCTGAGAGTGAGGTGCTGGAATCTTTCCCCGAAGAAGACTCT
  GTACTCAGCCATTCGTCCCTGAGCTCTCCCTCTTCCACCAGCTCGCCCGAGGGGCCGCCTGCTCCCCCAA
  AGCAGCACAGCAGTACCAGTCCCTTCCCCACCTCCACGCCCCTCCGCAGAATAACTAAGAAATTCGTCAA
  AGGCTCTACAGAGAAGAACAAGCTCAGACTGCAAAGAGATCAGGAGCGTCTGGGCAAGCAGCTCAAGTTA
  CGTGCAGAAAGGGAAGAAAAGGAGAAGCTGAAAGAGGAGGCCAAGCGGGCCAAGGAGGAGGCCAAGAAGA
  AGAAGGAGGAAGAGAAGGAGCTTAAGGAAAAGGAGAGGCGGGAGAAGCGGGAGAAGGATGAGAAGGAGAA
  GGCGGAGAAGCAGCGGCTCAAGGAGGAGCGGCGCAAGGAGAGACAGGAAGCCCTGGAGGCTAAACTTGAG
  GAAAAAAGGAAAAAGGAAGAAGAGAAACGGTTAAGAGAAGAAGAGAAGCGCATTAAAGCAGAGAAGGCCG
  AAATCACGAGGTTCTTCCAGAAACCAAAGACTCCACAGGCCCCCAAGACCCTGGCCGGCTCCTGTGGGAA
  GTTTGCCCCCTTTGAAATTAAAGAGCACATGGTCCTGGCCCCTCGGCGTCGGACCGCTTTCCATCCAGAC
  CTCTGCAGTCAGCTGGACCAGCTCCTCCAGCAGCAGAGCGGCGAGTTCTCCTTCTTGAAAGACCTCAAAG
  GCCGGCAGCCCCTGAGGTCCGGACCCACGCACGTTTCCACCCGGAATGCAGATATTTTTAACAGTGATGT
  CGTCATCGTGGAGCGTGGGAAGGGCGACGGTGTTCCCGAGAGGAGGAAGTTTGGCAGGATGAAGCTCCTG
  CAGTTCTGTGAGAACCACCGGCCTGCCTACTGGGGTACCTGGAATAAGAAGACGGCACTCATCCGCGCGC
  GAGACCCCTGGGCCCAGGACACGAAGCTCCTGGACTATGAGGTGGACAGTGATGAGGAGTGGGAAGAAGA
  GGAGCCTGGGGAGTCCCTGTCCCACAGTGAGGGGGATGATGATGACGACATGGGAGAGGATGAAGATGAG
  GACGATGGTTTCTTTGTGCCCCATGGGTACCTGTCTGAGGACGAAGGTGTGACAGAGGAGTGTGCCGACC
  CTGAGAACCATAAGGTCCGCCAGAAACTGAAGGCCAAGGAGTGGGACGAGTTCCTGGCTAAGGGGAAGCG
  CTTTCGCGTCCTGCAACCTGTGAAGATCGGCTGCGTGTGGGCGGCTGACAGAGACTGCGCAGGCGATGAC
  CTGAAGGTACTGCAGCAGTTCGCAGCCTGCTTCCTGGAGACCCTGCCGGCCCAGGAGGAGCAGACGCCCA
  AGGCCTCCAAGCGGGAGAGGAGAGACGAGCAGATCCTGGCCCAGCTGCTGCCGCTCCTGCACGGCAATGT
  GAACGGGAGCAAGGTCATCATCCGGGAGTTCCAGGAGCACTGCCGCCGGGGACTGCTCAGCAACCACACC
  GGCAGCCCGCGGAGCCCCTCCACCACCTACCTGCACACCCCCACCCCCAGCGAGGATGCCGCCATCCCCT
  CTAAGTCCCGGCTCAAGCGGCTCATTTCCGAGAACTCAGTGTATGAGAAGCGGCCTGACTTCAGGATGTG
  CTGGTACGTGCACCCGCAGGTGCTACAGAGCTTCCAGCAGGAGCACCTGCCCGTGCCGTGCCAGTGGAGC
  TATGTGACATCGGTGCCCTCGGCCCCCAAAGAGGACAGTGGCAGCGTCCCCTCCACGGGGCCCAGCCAGG
  GCACTCCCATCTCGCTGAAGAGGAAGTCAGCGGGCAGCATGTGCATCACCCAATTCATGAAGAAGCGCAG
  GCACGACGGCCAGATTGGTGCTGAAGACATGGACGGCTTCCAGGCAGACACGGAGGAGGAGGAAGAGGAG
  GAGGGCGACTGTATGATCGTGGATGTCCCGGATGCTGCGGAGGTCCAAGCCCCGTGTGGAGCCGCTTCCG
  GAGCTGGGGGTGGTGTGGGGGTGGACACCGGCAAGGCCACCCTGACCGCGAGCCCACTGGGTGCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209138 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNLVPKGKADDMSDDQGTSVQSKSPDLEA
  SLDTLENNCHVGSDIDFRPKLVNGKGPLDNFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEA
  SPSREAINGQREDTGDQQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQECSPRSCPEL
  TSGPRMCPRKEQDSWSEAGGILFKGKVPMVVLQDILAVRPPQIKSLPATPQGKNMTPESEVLESFPEEDS
  VLSHSSLSSPSSTSSPEGPPAPPKQHSSTSPFPTSTPLRRITKKFVKGSTEKNKLRLQRDQERLGKQLKL
  RAEREEKEKLKEEAKRAKEEAKKKKEEEKELKEKERREKREKDEKEKAEKQRLKEERRKERQEALEAKLE
  EKRKKEEEKRLREEEKRIKAEKAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMVLAPRRRTAFHPD
  LCSQLDQLLQQQSGEFSFLKDLKGRQPLRSGPTHVSTRNADIFNSDVVIVERGKGDGVPERRKFGRMKLL
  QFCENHRPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDSDEEWEEEEPGESLSHSEGDDDDDMGEDEDE
  DDGFFVPHGYLSEDEGVTEECADPENHKVRQKLKAKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGDD
  LKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLLPLLHGNVNGSKVIIREFQEHCRRGLLSNHT
  GSPRSPSTTYLHTPTPSEDAAIPSKSRLKRLISENSVYEKRPDFRMCWYVHPQVLQSFQQEHLPVPCQWS
  YVTSVPSAPKEDSGSVPSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGFQADTEEEEEE
  EGDCMIVDVPDAAEVQAPCGAASGAGGGVGVDTGKATLTASPLGAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005483
• ORF Size: 2868 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005483.2, NP_005474.2
• RefSeq Size: 3344 bp
• RefSeq ORF: 2871 bp
• Locus ID: 10036
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• MW: 106.9 kDa
• Gene Summary: Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]). [supplied by OMIM, Mar 2008]