CPSF2 (NM_017437) Human Tagged ORF Clone

CAT#: RC209857

CPSF2 (Myc-DDK-tagged)-Human cleavage and polyadenylation specific factor 2, 100kDa (CPSF2)

Product Images

Western blot validation of overexpression lysate (Cat# LY413751) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209857 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CPSF2 protein (Cat# TP309857). The protein was produced from HEK293T cells transfected with CPSF2 cDNA clone (Cat# RC209857) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPSF2
• Synonyms: CPSF100
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209857 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGTCTATTATCAAATTAACTACCCTTTCTGGGGTCCAAGAAGAATCTGCCCTTTGCTATCTTCTCC
  AAGTTGATGAGTTTAGATTTTTATTGGACTGTGGCTGGGATGAGCACTTTTCTATGGATATTATTGATTC
  CCTGAGGAAGCATGTTCACCAGATTGATGCAGTGCTGTTGTCTCACCCTGATCCTCTCCACCTTGGTGCC
  CTCCCGTATGCTGTCGGAAAGTTGGGTCTGAACTGTGCTATCTATGCAACCATTCCTGTTTATAAAATGG
  GACAGATGTTCATGTATGATCTTTATCAGTCTCGACACAATACAGAAGATTTTACACTCTTTACATTAGA
  TGATGTGGATGCAGCCTTTGATAAAATACAGCAGCTAAAATTCTCTCAGATTGTGAATTTGAAAGGTAAA
  GGACATGGCCTGTCTATCACACCTCTGCCAGCTGGTCATATGATAGGTGGAACAATATGGAAAATAGTCA
  AAGATGGAGAAGAAGAAATTGTTTATGCAGTTGACTTCAACCACAAGAGGGAGATCCATTTAAATGGATG
  TTCCCTGGAAATGCTAAGCAGGCCTTCCCTACTTATCACAGATTCATTCAATGCTACATATGTACAGCCT
  AGAAGAAAACAGAGAGATGAGCAGCTTCTGACAAATGTCCTGGAAACACTTCGAGGTGATGGAAATGTGT
  TAATAGCAGTGGACACAGCAGGCAGAGTTTTGGAACTTGCTCAACTTCTTGATCAGATTTGGAGGACTAA
  AGATGCAGGATTGGGTGTTTACTCATTGGCACTCCTAAATAATGTCAGTTACAATGTGGTGGAGTTTTCT
  AAGTCCCAGGTAGAATGGATGAGTGGTAAATTGATGAGATGTTTTGAAGACAAAAGAAATAATCCGTTTC
  AGTTTCGCCATCTCTCTTTATGTCATGGTCTTTCTGACTTGGCCCGTGTACCTAGCCCTAAAGTTGTACT
  TGCCAGCCAACCTGACCTGGAATGCGGATTTTCAAGGGATCTCTTTATTCAGTGGTGTCAGGACCCTAAA
  AACTCAATCATTCTAACCTACAGAACTACTCCTGGGACTTTAGCACGTTTCCTAATTGATAATCCTTCTG
  AAAAAATTACAGAAATAGAGTTGAGGAAACGTGTGAAGCTTGAAGGGAAAGAACTTGAAGAATACTTGGA
  AAAAGAGAAACTAAAGAAAGAAGCTGCCAAAAAGCTTGAGCAGTCAAAAGAGGCAGATATAGATTCCAGT
  GATGAGAGTGATATTGAGGAAGATATTGACCAGCCATCAGCTCATAAGACGAAGCATGACTTGATGATGA
  AAGGTGAAGGCAGTCGTAAAGGAAGTTTTTTCAAACAGGCAAAAAAGTCCTATCCTATGTTTCCTGCCCC
  AGAAGAAAGAATTAAATGGGATGAATATGGAGAGATTATCAAACCAGAGGATTTCTTAGTGCCAGAGCTT
  CAAGCTACTGAAGAAGAAAAAAGCAAATTAGAATCTGGTTTGACAAATGGAGATGAACCTATGGATCAGG
  ATTTATCTGATGTTCCTACTAAATGTATTTCTACAACAGAGTCTATTGAAATAAAAGCCCGGGTTACCTA
  CATAGACTATGAAGGACGCTCTGATGGGGATTCCATTAAAAAAATCATTAATCAGATGAAACCACGACAG
  TTGATCATCGTCCATGGCCCACCAGAGGCCAGTCAAGATCTGGCAGAGTGCTGTCGCGCCTTTGGTGGGA
  AAGATATTAAAGTGTACATGCCAAAGCTACATGAAACAGTTGATGCCACTAGTGAAACTCACATCTACCA
  GGTGAGGTTAAAAGACTCACTTGTCAGCTCTCTTCAGTTTTGTAAGGCAAAAGATGCTGAATTAGCTTGG
  ATAGATGGTGTCTTAGATATGAGAGTTTCCAAAGTGGACACAGGGGTTATTTTAGAAGAAGGAGAACTAA
  GGGATGATGGAGAAGACTCAGAGATGCAAGTGGAAGCTCCCTCAGATTCTAGCGTTATAGCACAACAAAA
  GGCCATGAAAAGTTTGTTCGGAGATGATGAAAAAGAAACAGGTGAAGAAAGTGAGATCATTCCTACTTTG
  GAACCCTTGCCACCTCATGAGGTTCCTGGACATCAGTCAGTTTTTATGAATGAACCAAGGCTGTCAGACT
  TCAAGCAAGTTCTCTTACGGGAGGGAATTCAAGCTGAATTTGTAGGAGGTGTACTTGTTTGCAACAATCA
  AGTAGCAGTCCGCAGAACGGAAACTGGACGCATTGGATTAGAAGGCTGCCTTTGTCAAGATTTTTATAGG
  ATAAGAGACCTTTTATATGAACAATATGCCATTGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209857 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTSIIKLTTLSGVQEESALCYLLQVDEFRFLLDCGWDEHFSMDIIDSLRKHVHQIDAVLLSHPDPLHLGA
  LPYAVGKLGLNCAIYATIPVYKMGQMFMYDLYQSRHNTEDFTLFTLDDVDAAFDKIQQLKFSQIVNLKGK
  GHGLSITPLPAGHMIGGTIWKIVKDGEEEIVYAVDFNHKREIHLNGCSLEMLSRPSLLITDSFNATYVQP
  RRKQRDEQLLTNVLETLRGDGNVLIAVDTAGRVLELAQLLDQIWRTKDAGLGVYSLALLNNVSYNVVEFS
  KSQVEWMSGKLMRCFEDKRNNPFQFRHLSLCHGLSDLARVPSPKVVLASQPDLECGFSRDLFIQWCQDPK
  NSIILTYRTTPGTLARFLIDNPSEKITEIELRKRVKLEGKELEEYLEKEKLKKEAAKKLEQSKEADIDSS
  DESDIEEDIDQPSAHKTKHDLMMKGEGSRKGSFFKQAKKSYPMFPAPEERIKWDEYGEIIKPEDFLVPEL
  QATEEEKSKLESGLTNGDEPMDQDLSDVPTKCISTTESIEIKARVTYIDYEGRSDGDSIKKIINQMKPRQ
  LIIVHGPPEASQDLAECCRAFGGKDIKVYMPKLHETVDATSETHIYQVRLKDSLVSSLQFCKAKDAELAW
  IDGVLDMRVSKVDTGVILEEGELRDDGEDSEMQVEAPSDSSVIAQQKAMKSLFGDDEKETGEESEIIPTL
  EPLPPHEVPGHQSVFMNEPRLSDFKQVLLREGIQAEFVGGVLVCNNQVAVRRTETGRIGLEGCLCQDFYR
  IRDLLYEQYAIV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017437
• ORF Size: 2346 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017437.1, NM_017437.2, NP_059133.1
• RefSeq Size: 5072 bp
• RefSeq ORF: 2349 bp
• Locus ID: 53981
• MW: 88.5 kDa