BDH2 (NM_020139) Human Tagged ORF Clone

CAT#: RC210586

BDH2 (Myc-DDK-tagged)-Human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2)

Product Images

Western blot validation of overexpression lysate (Cat# LY402755) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210586 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BDH2 protein (Cat# TP310586). The protein was produced from HEK293T cells transfected with BDH2 cDNA clone (Cat# RC210586) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BDH2
• Synonyms: DHRS6; EFA6R; PRO20933; SDR15C1; UCPA-OR; UNQ6308
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210586 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCGACTTGATGGGAAAGTCATCATCCTGACGGCCGCTGCTCAGGGGATTGGCCAAGCAGCTGCCT
  TAGCTTTTGCAAGAGAAGGTGCCAAAGTCATAGCCACAGACATTAATGAGTCCAAACTTCAGGAACTGGA
  AAAGTACCCGGGTATTCAAACTCGTGTCCTTGATGTCACAAAGAAGAAACAAATTGATCAGTTTGCCAAT
  GAAGTTGAGAGACTTGATGTTCTCTTTAATGTTGCTGGTTTTGTCCATCATGGAACTGTCCTGGATTGTG
  AGGAGAAAGACTGGGACTTCTCGATGAATCTCAATGTGCGCAGCATGTACCTGATGATCAAGGCATTCCT
  TCCTAAAATGCTTGCTCAGAAATCTGGCAATATTATCAACATGTCTTCTGTGGCTTCCAGCGTCAAAGGA
  GTTGTGAACAGATGTGTGTACAGCACAACCAAGGCAGCCGTGATTGGCCTCACAAAATCTGTGGCTGCAG
  ATTTCATCCAGCAGGGCATCAGGTGCAACTGTGTGTGCCCAGGAACAGTTGATACGCCATCTCTACAAGA
  AAGAATACAAGCCAGAGGAAATCCTGAAGAGGCACGGAATGATTTCCTGAAGAGACAAAAGACGGGAAGA
  TTCGCAACTGCAGAAGAAATAGCCATGCTCTGCGTGTATTTGGCTTCTGATGAATCTACTTATGTAACTG
  GTAACCCTGTCATCATTGATGGAGGCTGGAGCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210586 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRLDGKVIILTAAAQGIGQAAALAFAREGAKVIATDINESKLQELEKYPGIQTRVLDVTKKKQIDQFAN
  EVERLDVLFNVAGFVHHGTVLDCEEKDWDFSMNLNVRSMYLMIKAFLPKMLAQKSGNIINMSSVASSVKG
  VVNRCVYSTTKAAVIGLTKSVAADFIQQGIRCNCVCPGTVDTPSLQERIQARGNPEEARNDFLKRQKTGR
  FATAEEIAMLCVYLASDESTYVTGNPVIIDGGWSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020139
• ORF Size: 735 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020139.1, NM_020139.2, NM_020139.3, NP_064524.3
• RefSeq Size: 2936 bp
• RefSeq ORF: 738 bp
• Locus ID: 56898
• Domains: adh_short
• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Metabolic pathways, Synthesis and degradation of ketone bodies
• MW: 26.8 kDa
• Gene Summary: Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. Also acts as a 3-hydroxybutyrate dehydrogenase. [UniProtKB/Swiss-Prot Function]