Rho GTPase activating protein 29 (ARHGAP29) (NM_004815) Human Tagged ORF Clone

CAT#: RC210830

ARHGAP29 (Myc-DDK-tagged)-Human Rho GTPase activating protein 29 (ARHGAP29)

Product Images

Western blot validation of overexpression lysate (Cat# LY417731) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210830 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ARHGAP29 protein (Cat# TP310830). The protein was produced from HEK293T cells transfected with ARHGAP29 cDNA clone (Cat# RC210830) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGAP29
• Synonyms: PARG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210830 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGCTCACAAACAGAAAAAGACAAAGAAAAAACGTGCTTGGGCATCAGGTCAACTCTCTACTGATA
  TTACAACTTCTGAAATGGGGCTCAAGTCCTTAAGTTCCAACTCTATTTTTGATCCGGATTACATCAAGGA
  GTTGGTGAATGATATCAGGAAGTTCTCCCACATGTTACTATATTTGAAAGAAGCCATATTTTCAGACTGT
  TTTAAAGAAGTTATTCATATACGTCTAGAGGAACTGCTCCGTGTTTTAAAGTCTATAATGAATAAACATC
  AGAACCTCAATTCTGTTGATCTTCAAAATGCTGCAGAAATGCTCACTGCAAAAGTGAAAGCTGTGAACTT
  CACAGAAGTTAATGAAGAAAACAAAAACGATCTCTTCCAGGAAGTGTTTTCTTCTATTGAAACTTTGGCA
  TTTACCTTTGGAAATATCCTTACAAACTTCCTTATGGGAGATGTAGGCAATGATTCATTATTGCGACTGC
  CTGTTTCTCGAGAAACTAAGTCGTTTGAAAATGTTTCTGTGGAATCAGTGGACTCATCCAGTGAAAAAGG
  AAATTTTTCCCCTTTAGAACTAGACAACGTGCTGTTAAAGAACACTGACTCTATCGAGCTGGCTTTGTCA
  TATGCTAAAACTTGGTCAAAATATACTAAGAACATAGTTTCATGGGTTGAAAAAAAGCTTAACTTGGAAT
  TGGAGTCCACTAGAAATATGGTCAAGTTGGCAGAGGCAACTAGAACTAACATTGGAATTCAGGAGTTCAT
  GCCACTGCAGTCTCTGTTTACTAATGCTCTTCTTAATGATATAGAAAGCAGTCACCTTTTACAACAAACA
  ATTGCAGCTCTCCAGGCTAACAAATTTGTGCAGCCTCTACTTGGAAGGAAAAATGAAATGGAAAAACAAA
  GGAAAGAAATAAAAGAGCTTTGGAAACAGGAGCAAAATAAAATGCTTGAAGCAGAGAATGCTCTCAAAAA
  GGCAAAATTATTATGCATGCAACGTCAAGATGAATATGAGAAAGCAAAGTCTTCCATGTTTCGTGCAGAA
  GAGGAGCATCTGTCTTCAAGTGGCGGATTAGCAAAAAATCTCAACAAGCAACTAGAAAAAAAGCGAAGGT
  TGGAAGAGGAGGCTCTCCAAAAAGTAGAAGAAGCAAATGAACTTTACAAAGTTTGTGTGACAAATGTTGA
  AGAAAGAAGAAATGATCTAGAAAATACCAAAAGAGAAATTTTAGCACAACTCCGGACACTTGTTTTCCAG
  TGTGATCTTACCCTTAAAGCTGTAACAGTTAACCTCTTCCACATGCAGCATCTGCAGGCTGCTTCCCTTG
  CAGACAGTTTACAGTCTCTCTGTGATAGTGCCAAACTCTATGACCCAGGCCAAGAGTACAGTGAATTTGT
  CAAGGCCACAAATTCAACTGAAGAAGAAAAAGTTGATGGAAATGTAAATAAACATTTAAATAGTTCCCAA
  CCTTCAGGATTTGGACCTGCCAACTCTTTAGAGGATGTTGTACGCCTTCCTGACAGTTCTAATAAAATTG
  AAGAGGACAGATGCTCTAACAGTGCAGATATAACAGGTCCTTCCTTTATAAGATCATGGACATTTGGGAT
  GTTTAGTGATTCTGAGAGCACTGGAGGGAGCAGCGAATCTAGATCTCTGGATTCAGAATCTATAAGTCCA
  GGAGACTTTCATCGAAAACTTCCACGAACACCATCCAGTGGAACTATGTCCTCTGCAGATGATCTAGATG
  AAAGAGAGCCACCTTCCCCTTCAGAAACTGGACCCAATTCCCTTGGAACATTTAAGAAAACATTGATGTC
  AAAGGCAGCTCTCACACACAAGTTTCGCAAATTGAGATCCCCCACGAAATGTAGGGATTGTGAAGGCATT
  GTAGTGTTCCAAGGTGTTGAATGTGAAGAGTGTCTCCTTGTTTGTCATCGAAAGTGTTTGGAAAATTTAG
  TCATTATTTGTGGTCATCAGAAACTTCCAGGAAAAATACACTTATTTGGAGCAGAATTCACACAAGTTGC
  AAAAAAGGAACCAGATGGTATCCCTTTTATACTCAAAATATGTGCCTCAGAGATTGAAAATAGAGCTTTG
  TGTCTACAGGGAATTTATCGTGTGTGTGGAAACAAAATAAAAACTGAAAAATTGTGTCAAGCTTTGGAAA
  ATGGAATGCACTTGGTAGATATTTCAGAATTTAGTTCACATGATATCTGTGACGTCTTGAAATTATACCT
  TCGGCAGCTCCCAGAACCATTTATTTTATTTCGATTGTACAAGGAATTTATAGACCTTGCAAAAGAGATC
  CAACATGTAAATGAAGAACAAGAGACAAAAAAGAATAGTCTTGAAGACAAAAAATGGCCAAATATGTGTA
  TAGAAATAAACCGAATTCTTCTAAAAAGCAAAGACCTTCTAAGACAATTGCCAGCATCAAATTTTAACAG
  TCTTCATTTCCTTATAGTACATCTAAAGCGGGTAGTAGATCATGCAGAAGAAAACAAGATGAACTCCAAA
  AACTTGGGGGTGATATTTGGACCAAGTCTCATTAGGCCAAGGCCCACAACTGCTCCTATCACCATCTCCT
  CCCTTGCAGAGTATTCAAATCAAGCACGCTTGGTAGAGTTTCTCATTACTTACTCACAGAAGATCTTCGA
  TGGGTCCCTACAACCACAAGATGTTATGTGTAGCATAGGTGTTGTTGATCAAGGCTGTTTTCCAAAGCCT
  CTGTTATCACCAGAAGAAAGAGACATTGAACGTTCCATGAAGTCACTATTTTTTTCTTCAAAGGAAGATA
  TCCATACTTCAGAGAGTGAAAGCAAAATTTTTGAACGAGCTACATCATTTGAGGAATCAGAACGCAAGCA
  AAATGCGTTAGGAAAATGTGATGCATGTCTCAGTGACAAAGCACAGTTGCTTCTAGACCAAGAGGCTGAA
  TCAGCATCCCAAAAGATAGAAGATGGTAAAACCCCTAAGCCACTTTCTCTGAAATCTGATAGGTCAACAA
  ACAATGTGGAGAGGCATACTCCAAGGACCAAGATTAGACCTGTAAGTTTGCCTGTAGATAGACTACTTCT
  TGCAAGTCCTCCTAATGAGAGAAATGGCAGAAATATGGGAAATGTAAATTTAGACAAGTTTTGCAAGAAT
  CCTGCCTTTGAAGGAGTTAATAGAAAAGACGCTGCTACTACTGTTTGTTCCAAATTTAATGGCTTTGACC
  AGCAAACTCTACAGAAAATTCAGGACAAACAGTATGAACAAAACAGCCTAACTGCCAAGACTACAATGAT
  CATGCCCAGTGCACTCCAGGAAAAAGGAGTGACAACAAGCCTCCAGATTAGTGGGGACCATTCTATCAAT
  GCCACTCAACCCAGTAAGCCATATGCAGAGCCAGTCAGGTCAGTGAGAGAGGCATCTGAGAGACGGTCTT
  CAGATTCCTACCCTCTCGCTCCTGTCAGAGCACCCAGAACACTGCAGCCTCAACATTGGACAACATTTTA
  TAAACCACATGCTCCCATCATCAGTATCAGGGGGAATGAGGAGAAGCCAGCTTCACCCTCAGCAGCAGTG
  CCTCCTGGCACAGATCACGATCCCCACGGTCTCGTGGTGAAGTCAATGCCAGACCCAGACAAAGCATCAG
  CTTGTCCTGGGCAAGCAACTGGTCAACCTAAAGAAGACTCTGAGGAGCTTGGCTTGCCTGATGTGAATCC
  AATGTGTCAGAGACCAAGGCTAAAACGAATGCAACAGTTTGAAGACCTCGAAGATGAAATTCCACAATTT
  GTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210830 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIAHKQKKTKKKRAWASGQLSTDITTSEMGLKSLSSNSIFDPDYIKELVNDIRKFSHMLLYLKEAIFSDC
  FKEVIHIRLEELLRVLKSIMNKHQNLNSVDLQNAAEMLTAKVKAVNFTEVNEENKNDLFQEVFSSIETLA
  FTFGNILTNFLMGDVGNDSLLRLPVSRETKSFENVSVESVDSSSEKGNFSPLELDNVLLKNTDSIELALS
  YAKTWSKYTKNIVSWVEKKLNLELESTRNMVKLAEATRTNIGIQEFMPLQSLFTNALLNDIESSHLLQQT
  IAALQANKFVQPLLGRKNEMEKQRKEIKELWKQEQNKMLEAENALKKAKLLCMQRQDEYEKAKSSMFRAE
  EEHLSSSGGLAKNLNKQLEKKRRLEEEALQKVEEANELYKVCVTNVEERRNDLENTKREILAQLRTLVFQ
  CDLTLKAVTVNLFHMQHLQAASLADSLQSLCDSAKLYDPGQEYSEFVKATNSTEEEKVDGNVNKHLNSSQ
  PSGFGPANSLEDVVRLPDSSNKIEEDRCSNSADITGPSFIRSWTFGMFSDSESTGGSSESRSLDSESISP
  GDFHRKLPRTPSSGTMSSADDLDEREPPSPSETGPNSLGTFKKTLMSKAALTHKFRKLRSPTKCRDCEGI
  VVFQGVECEECLLVCHRKCLENLVIICGHQKLPGKIHLFGAEFTQVAKKEPDGIPFILKICASEIENRAL
  CLQGIYRVCGNKIKTEKLCQALENGMHLVDISEFSSHDICDVLKLYLRQLPEPFILFRLYKEFIDLAKEI
  QHVNEEQETKKNSLEDKKWPNMCIEINRILLKSKDLLRQLPASNFNSLHFLIVHLKRVVDHAEENKMNSK
  NLGVIFGPSLIRPRPTTAPITISSLAEYSNQARLVEFLITYSQKIFDGSLQPQDVMCSIGVVDQGCFPKP
  LLSPEERDIERSMKSLFFSSKEDIHTSESESKIFERATSFEESERKQNALGKCDACLSDKAQLLLDQEAE
  SASQKIEDGKTPKPLSLKSDRSTNNVERHTPRTKIRPVSLPVDRLLLASPPNERNGRNMGNVNLDKFCKN
  PAFEGVNRKDAATTVCSKFNGFDQQTLQKIQDKQYEQNSLTAKTTMIMPSALQEKGVTTSLQISGDHSIN
  ATQPSKPYAEPVRSVREASERRSSDSYPLAPVRAPRTLQPQHWTTFYKPHAPIISIRGNEEKPASPSAAV
  PPGTDHDPHGLVVKSMPDPDKASACPGQATGQPKEDSEELGLPDVNPMCQRPRLKRMQQFEDLEDEIPQF
  V
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004815
• ORF Size: 3783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004815.2, NM_004815.3, NP_004806.2
• RefSeq Size: 9121 bp
• RefSeq ORF: 3786 bp
• Locus ID: 9411
• Domains: RhoGAP, DAG_PE-bind
• MW: 142.1 kDa
• Gene Summary: Rap1 is a small GTPase that, through effectors, regulates Rho GTPase signaling. These effectors- Rasip1, Radil, and the protein encoded by this gene- translocate to the cell membrane, where they form a multiprotein complex. This complex is necessary for Rap1-induced inhibition of Rho signaling. Defects in this gene may be a cause of nonsyndromic cleft lip with or without cleft palate. [provided by RefSeq, Jun 2016]