SPC24 (NM_182513) Human Tagged ORF Clone
CAT#: RC211241
SPC24 (Myc-DDK-tagged)-Human SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (SPC24)
"NM_182513" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SPC24 |
Synonyms | SPBC24 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC211241 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCTTCCGCGACATAGAGGAGGTGAGCCAGGGGCTGCTCAGCCTGCTGGGCGCCAACCGCGCGG AGGCGCAGCAGCGACGGCTGCTGGGGCGCCACGAGCAGGTGGTGGAGCGGCTGCTGGAAACGCAAGACGG TGCCGAGAAGCAGCTGCGAGAGATCCTCACCATGGAGAAGGAAGTGGCCCAGAGCCTTCTCAATGCGAAG GAGCAGGTGCACCAGGGAGGCGTGGAGCTGCAGCAGCTGGAAGCTGGGCTTCAGGAGGCTGGGGAGGAGG ACACCCGTCTGAAGGCCAGCCTCCTTCAGCTCACCAGAGAGCTGGAAGAGCTCAAGGAGATTGAGGCGGA TCTGGAGCGACAGGAGAAGGAGGTCGACGAGGACACGACAGTCACAATCCCCTCGGCCGTGTACGTGGCT CAACTTTACCACCAAGTTAGTAAAATTGAGTGGGATTATGAGTGTGAGCCAGGGATGGTCAAAGGCATCC ATCATGGCCCCAGTGTGGCCCAGCCCATCCACCTGGACAGCACCCAGCTCTCCAGGAAATTCATCAGCGA CTACCTCTGGAGTCTGGTGGACACCGAGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211241 protein sequence
Red=Cloning site Green=Tags(s) MAAFRDIEEVSQGLLSLLGANRAEAQQRRLLGRHEQVVERLLETQDGAEKQLREILTMEKEVAQSLLNAK EQVHQGGVELQQLEAGLQEAGEEDTRLKASLLQLTRELEELKEIEADLERQEKEVDEDTTVTIPSAVYVA QLYHQVSKIEWDYECEPGMVKGIHHGPSVAQPIHLDSTQLSRKFISDYLWSLVDTEW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_182513 |
ORF Size | 591 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_182513.1, NM_182513.2, NM_182513.3, NP_872319.1 |
RefSeq Size | 654 bp |
RefSeq ORF | 594 bp |
Locus ID | 147841 |
Protein Families | Druggable Genome |
MW | 22.4 kDa |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC307413 | SPC24 (untagged)-Human SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (SPC24) |
USD 420.00 |
|
RG211241 | SPC24 (GFP-tagged) - Human SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (SPC24) |
USD 460.00 |
|
RC211241L3 | Lenti ORF clone of Human SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (SPC24), Myc-DDK-tagged |
USD 620.00 |
|
RC211241L4 | Lenti ORF clone of Human SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (SPC24), mGFP tagged |
USD 620.00 |
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