REST (NM_005612) Human Tagged ORF Clone

CAT#: RC211570

REST (Myc-DDK-tagged)-Human RE1-silencing transcription factor (REST), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: REST
• Synonyms: DFNA27; GINGF5; HGF5; NRSF; WT6; XBR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211570 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCCAGGTAATGGGGCAGTCTTCTGGAGGAGGAGGGCTGTTTACCAGCAGTGGCAACATTGGAA
  TGGCCCTGCCTAACGACATGTATGACTTGCATGACCTTTCCAAAGCTGAACTGGCCGCACCTCAGCTTAT
  TATGCTGGCAAATGTGGCCTTAACTGGGGAAGTAAATGGCAGCTGCTGTGATTACCTGGTCGGTGAAGAA
  AGACAGATGGCAGAACTGATGCCGGTTGGGGATAACAACTTTTCAGATAGTGAAGAAGGAGAAGGACTTG
  AAGAGTCTGCTGATATAAAAGGTGAACCTCATGGACTGGAAAACATGGAACTGAGAAGTTTGGAACTCAG
  CGTCGTAGAACCTCAGCCTGTATTTGAGGCATCAGGTGCTCCAGATATTTACAGTTCAAATAAAGATCTT
  CCCCCTGAAACACCTGGAGCGGAGGACAAAGGCAAGAGCTCGAAGACCAAACCCTTTCGCTGTAAGCCAT
  GCCAATATGAAGCAGAATCTGAAGAACAGTTTGTGCATCACATCAGAGTTCACAGTGCTAAGAAATTTTT
  TGTGGAAGAGAGTGCAGAGAAGCAGGCAAAAGCCAGGGAATCTGGCTCTTCCACTGCAGAAGAGGGAGAT
  TTCTCCAAGGGCCCCATTCGCTGTGACCGCTGCGGCTACAATACTAATCGATATGATCACTATACAGCAC
  ACCTGAAACACCACACCAGAGCTGGGGATAATGAGCGAGTCTACAAGTGTATCATTTGCACATACACAAC
  AGTGAGCGAGTATCACTGGAGGAAACATTTAAGAAACCATTTTCCAAGGAAAGTATACACATGTGGAAAA
  TGCAACTATTTTTCAGACAGAAAAAACAATTATGTTCAGCATGTTAGAACTCATACAGGAGAACGCCCAT
  ATAAATGTGAACTTTGTCCTTACTCAAGTTCTCAGAAGACTCATCTAACTAGACATATGCGTACTCATTC
  AGGTGAGAAGCCATTTAAATGTGATCAGTGCAGTTATGTGGCCTCTAATCAACATGAAGTAACCCGCCAT
  GCAAGACAGGTTCACAATGGGCCTAAACCTCTTAATTGCCCACACTGTGATTACAAAACAGCAGATAGAA
  GCAACTTCAAAAAACATGTAGAGCTACATGTGAACCCACGGCAGTTCAATTGCCCTGTATGTGACTATGC
  AGCTTCCAAGAAGTGTAATCTACAGTATCACTTCAAATCTAAGCATCCTACTTGTCCTAATAAAACAATG
  GATGTCTCAAAAGTGAAACTAAAGAAAACCAAAAAACGAGAGGCTGACTTGCCTGATAATATTACCAATG
  AAAAAACAGAAATAGAACAAACAAAAATAAAAGGGGATGTGGCTGGAAAGAAAAATGAAAAGTCCGTCAA
  AGCAGAGAAAAGAGATGTCTCAAAAGAGAAAAAGCCTTCTAATAATGTGTCAGTGATCCAGGTGACTACC
  AGAACTCGAAAATCAGTAACAGAGGTGAAAGAGATGGATGTGCATACAGGAAGCAATTCAGAAAAATTCA
  GTAAAACTAAGAAAAGCAAAAGGAAGCTGGAAGTTGACAGCCATTCTTTACATGGTCCTGTGAATGATGA
  GGAATCTTCAACAAAAAAGAAAAAGAAGGTAGAAAGCAAATCCAAAAATAATAGTCAGGAAGTGCCAAAG
  GGTGACAGCAAAGTGGAGGAGAATAAAAAGCAAAATACTTGCATGAAAAAAAGTACAAAGAAGAAAACTC
  TGAAAAATAAATCAAGTAAGAAAAGCAGTAAGCCTCCTCAGAAGGAACCTGTTGAGAAGGGATCTGCTCA
  GATGGACCCTCCTCAGATGGGGCCTGCTCCCACAGAGGCGGTTCAGAAGGGGCCCATTCAGGTGGAGCCG
  CCACCTCCCATGGAGCATGCTCAGATGGAGGGTGCCCAGATACGGCCTGCTCCTGACGAGCCTGTTCAGA
  TGGAGGTGGTTCAGGAGGGGCCTGCTCAGAAGGAGCTGCTGCCTCCCGTGGAGCCTGCTCAGATGGTGGG
  TGCCCAAATTGTACTTGCTCACATGGAGCTGCCTCCTCCCATGGAGACTGCTCAGACGGAGGTTGCCCAA
  ATGGGGCCTGCTCCCATGGAACCTGCTCAGATGGAGGTTGCCCAGGTAGAATCTGCTCCCATGCAGGTGG
  TCCAGAAGGAGCCTGTTCAGATGGAGCTGTCTCCTCCCATGGAGGTGGTCCAGAAGGAGCCTGTTCAGAT
  AGAGCTGTCTCCTCCCATGGAGGTGGTCCAGAAGGAACCTGTTAAGATAGAGCTGTCTCCTCCCATAGAG
  GTGGTCCAGAAGGAGCCTGTTCAGATGGAGTTGTCTCCTCCCATGGGGGTGGTTCAGAAGGAGCCTGCTC
  AGAGGGAGCCACCTCCTCCCAGAGAGCCTCCCCTTCACATGGAGCCAATTTCCAAAAAGCCTCCTCTCCG
  AAAAGATAAAAAGGAAAAGTCTAACATGCAGAGTGAAAGGGCACGGAAGGAGCAAGTCCTTATTGAAGTT
  GGCTTAGTGCCTGTTAAAGATAGCTGGCTTCTAAAGGAAAGTGTAAGCACAGAGGATCTCTCACCACCAT
  CACCACCACTGCCAAAGGAAAATTTAAGAGAAGAGGCATCAGGAGACCAAAAATTACTCAACACAGGTGA
  AGGAAATAAAGAAGCCCCTCTTCAGAAAGTAGGAGCAGAAGAGGCAGATGAGAGCCTACCTGGTCTTGCT
  GCTAATATCAACGAATCTACCCATATTTCATCCTCTGGACAAAACTTGAATACGCCAGAGGGTGAAACTT
  TAAATGGTAAACATCAGACTGACAGTATAGTTTGTGAAATGAAAATGGACACTGATCAGAACACAAGAGA
  GAATCTCACTGGTATAAATTCAACAGTTGAAGAACCAGTTTCACCAATGCTTCCCCCTTCAGCAGTAGAA
  GAACGTGAAGCAGTGTCCAAAACTGCACTGGCATCACCTCCTGCTACAATGGCAGCAAATGAGTCTCAGG
  AAATTGATGAAGATGAAGGCATCCACAGCCATGAAGGAAGTGACCTAAGTGACAACATGTCAGAGGGTAG
  TGATGATTCTGGATTGCATGGGGCTCGGCCAGTTCCACAAGAATCTAGCAGAAAAAATGCAAAGGAAGCG
  TTGGCAGTCAAAGCGGCTAAGGGAGATTTTGTTTGTATCTTCTGTGATCGTTCTTTCAGAAAGGGAAAAG
  ATTACAGCAAACACCTCAATCGCCATTTGGTTAATGTGTACTATCTTGAAGAAGCAGCTCAAGGGCAGGA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211570 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATQVMGQSSGGGGLFTSSGNIGMALPNDMYDLHDLSKAELAAPQLIMLANVALTGEVNGSCCDYLVGEE
  RQMAELMPVGDNNFSDSEEGEGLEESADIKGEPHGLENMELRSLELSVVEPQPVFEASGAPDIYSSNKDL
  PPETPGAEDKGKSSKTKPFRCKPCQYEAESEEQFVHHIRVHSAKKFFVEESAEKQAKARESGSSTAEEGD
  FSKGPIRCDRCGYNTNRYDHYTAHLKHHTRAGDNERVYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCGK
  CNYFSDRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCSYVASNQHEVTRH
  ARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPNKTM
  DVSKVKLKKTKKREADLPDNITNEKTEIEQTKIKGDVAGKKNEKSVKAEKRDVSKEKKPSNNVSVIQVTT
  RTRKSVTEVKEMDVHTGSNSEKFSKTKKSKRKLEVDSHSLHGPVNDEESSTKKKKKVESKSKNNSQEVPK
  GDSKVEENKKQNTCMKKSTKKKTLKNKSSKKSSKPPQKEPVEKGSAQMDPPQMGPAPTEAVQKGPIQVEP
  PPPMEHAQMEGAQIRPAPDEPVQMEVVQEGPAQKELLPPVEPAQMVGAQIVLAHMELPPPMETAQTEVAQ
  MGPAPMEPAQMEVAQVESAPMQVVQKEPVQMELSPPMEVVQKEPVQIELSPPMEVVQKEPVKIELSPPIE
  VVQKEPVQMELSPPMGVVQKEPAQREPPPPREPPLHMEPISKKPPLRKDKKEKSNMQSERARKEQVLIEV
  GLVPVKDSWLLKESVSTEDLSPPSPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVGAEEADESLPGLA
  ANINESTHISSSGQNLNTPEGETLNGKHQTDSIVCEMKMDTDQNTRENLTGINSTVEEPVSPMLPPSAVE
  EREAVSKTALASPPATMAANESQEIDEDEGIHSHEGSDLSDNMSEGSDDSGLHGARPVPQESSRKNAKEA
  LAVKAAKGDFVCIFCDRSFRKGKDYSKHLNRHLVNVYYLEEAAQGQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005612
• ORF Size: 3291 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005612.2, NM_005612.3, NM_005612.4, NP_005603.2
• RefSeq Size: 7333 bp
• RefSeq ORF: 3294 bp
• Locus ID: 5978
• Cytogenetics: 4q12
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease
• MW: 121.9 kDa
• Gene Summary: 'This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018]'