XYLB (NM_005108) Human Tagged ORF Clone

CAT#: RC212021

XYLB (Myc-DDK-tagged)-Human xylulokinase homolog (H. influenzae) (XYLB)

Product Images

Western blot validation of overexpression lysate (Cat# LY417522) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212021 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified XYLB protein (Cat# TP312021). The protein was produced from HEK293T cells transfected with XYLB cDNA clone (Cat# RC212021) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: XYLB
• Synonyms: FLJ10343; FLJ12539; FLJ22075
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212021 representing NM_005108
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCACGCCCCTCGCCGCTGCTGCCTGGGCTGGGACTTCAGCACGCAGCAGGTAAAGGTTGTTG
  CTGTTGATGCAGAGTTGAATGTCTTCTATGAGGAAAGTGTGCATTTTGACAGAGATCTTCCAGAATTTGG
  GACTCAGGGCGGTGTTCATGTGCACAAGGATGGGCTGACGGTCACTTCTCCAGTACTAATGTGGGTCCAG
  GCACTGGATATCATCTTGGAGAAGATGAAGGCTTCGGGCTTCGACTTCTCTCAAGTCCTAGCCTTGTCCG
  GGGCGGGCCAGCAACACGGAAGTATATACTGGAAGGCTGGAGCCCAGCAGGCACTGACAAGCTTATCACC
  AGACCTCCGGCTACACCAGCAGCTGCAGGACTGTTTCTCCATCAGCGACTGCCCGGTGTGGATGGACTCC
  AGCACCACAGCCCAGTGCCGCCAGCTGGAGGCTGCTGTGGGTGGTGCTCAGGCTCTCAGCTGCCTCACGG
  GGTCCCGTGCCTATGAGCGTTTTACAGGGAACCAAATTGCAAAAATTTACCAGCAGAACCCCGAGGCCTA
  CTCACATACGGAGAGAATTTCTTTGGTCAGTAGCTTTGCTGCTTCCCTGTTCCTTGGCTCTTACTCCCCT
  ATTGACTACAGTGATGGTTCTGGAATGAATTTGTTGCAGATACAGGATAAAGTCTGGTCCCAGGCTTGCC
  TTGGTGCCTGTGCACCTCATTTAGAGGAGAAGCTTAGCCCACCAGTACCATCATGCTCAGTTGTGGGAGC
  CATTTCTTCCTACTACGTCCAGCGCTACGGATTTCCTCCAGGATGCAAAGTGGTGGCCTTCACTGGGGAC
  AACCCAGCGTCGCTGGCAGGCATGAGACTGGAGGAAGGTGACATTGCGGTCAGCCTGGGCACCAGTGACA
  CCCTGTTTCTCTGGCTCCAAGAGCCCATGCCTGCCCTGGAAGGCCACATCTTCTGCAACCCGGTTGACTC
  CCAGCACTACATGGCACTCCTGTGCTTTAAAAATGGCTCCCTCATGAGAGAGAAGATCCGCAACGAGTCT
  GTATCCCGTTCCTGGAGCGATTTCTCTAAGGCACTGCAGTCCACAGAGATGGGCAACGGTGGAAACCTGG
  GTTTTTATTTTGATGTAATGGAGATCACCCCTGAAATTATTGGACGTCATAGGTTTAACACAGAAAACCA
  CAAGGTTGCAGCATTCCCTGGGGATGTGGAGGTTCGAGCACTAATTGAAGGACAATTCATGGCCAAGAGG
  ATTCACGCAGAAGGCCTGGGCTATCGAGTCATGTCCAAGACAAAGATTTTGGCCACAGGAGGAGCATCTC
  ACAATAGAGAAATCTTACAGGTGCTTGCAGATGTGTTTGATGCCCCGGTGTATGTTATAGACACTGCCAA
  CTCGGCCTGTGTGGGTTCTGCATACCGAGCTTTTCATGGTCTTGCAGGTGGAACAGATGTGCCCTTTTCA
  GAGGTTGTGAAGTTAGCTCCAAATCCCAGACTAGCTGCTACCCCAAGCCCGGGAGCTTCTCAGGTCTACG
  AGGCCCTTCTCCCCCAGTATGCCAAACTCGAGCAGAGAATCTTGTCTCAGACCCGGGGGCCTCCGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212021 representing NM_005108
  Red=Cloning site Green=Tags(s)
  
  MAEHAPRRCCLGWDFSTQQVKVVAVDAELNVFYEESVHFDRDLPEFGTQGGVHVHKDGLTVTSPVLMWVQ
  ALDIILEKMKASGFDFSQVLALSGAGQQHGSIYWKAGAQQALTSLSPDLRLHQQLQDCFSISDCPVWMDS
  STTAQCRQLEAAVGGAQALSCLTGSRAYERFTGNQIAKIYQQNPEAYSHTERISLVSSFAASLFLGSYSP
  IDYSDGSGMNLLQIQDKVWSQACLGACAPHLEEKLSPPVPSCSVVGAISSYYVQRYGFPPGCKVVAFTGD
  NPASLAGMRLEEGDIAVSLGTSDTLFLWLQEPMPALEGHIFCNPVDSQHYMALLCFKNGSLMREKIRNES
  VSRSWSDFSKALQSTEMGNGGNLGFYFDVMEITPEIIGRHRFNTENHKVAAFPGDVEVRALIEGQFMAKR
  IHAEGLGYRVMSKTKILATGGASHNREILQVLADVFDAPVYVIDTANSACVGSAYRAFHGLAGGTDVPFS
  EVVKLAPNPRLAATPSPGASQVYEALLPQYAKLEQRILSQTRGPPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005108
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005108.2, NM_005108.3, NP_005099.2
• RefSeq Size: 3694
• RefSeq ORF: 1611
• Locus ID: 9942
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pentose and glucuronate interconversions
• MW: 58.2 kDa
• Gene Summary: The protein encoded by this gene shares 22% sequence identity with Hemophilus influenzae xylulokinase, and even higher identity to other gene products in C.elegans (45%) and yeast (31-35%), which are thought to belong to a family of enzymes that include fucokinase, gluconokinase, glycerokinase and xylulokinase. These proteins play important roles in energy metabolism. [provided by RefSeq, Aug 2009]