SERPINB8 (NM_198833) Human Tagged ORF Clone

CAT#: RC212081

SERPINB8 (Myc-DDK-tagged)-Human serpin peptidase inhibitor, clade B (ovalbumin), member 8 (SERPINB8), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY404778) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212081 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SERPINB8
• Synonyms: C18orf53; CAP2; PI-8; PI8; PSS5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212081 representing NM_198833
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGACCTCTGTGAAGCAAATGGCACTTTTGCCATCAGCTTATTTAAAATATTGGGGGAAGAGGACA
  ACTCAAGAAACGTATTCTTCTCTCCCATGAGCATCTCCTCTGCCCTGGCCATGGTCTTCATGGGGGCAAA
  GGGAAGCACTGCAGCCCAGATGTCCCAGGCACTTTGTTTATACAAAGACGGAGATATTCACCGAGGTTTC
  CAGTCACTTCTCAGTGAAGTTAACAGAACTGGCACTCAGTACTTGCTTAGAACTGCCAACAGACTCTTTG
  GAGAAAAGACGTGTGATTTCCTTCCAGACTTTAAAGAATACTGTCAGAAGTTCTATCAGGCAGAGCTGGA
  GGAGTTGTCCTTTGCTGAAGACACTGAAGAGTGCAGGAAGCATATAAATGACTGGGTGGCAGAGAAGACT
  GAAGGTAAGATTTCAGAGGTACTGGATGCTGGGACAGTCGATCCCCTGACAAAGCTGGTCCTTGTGAATG
  CCATTTATTTCAAGGGAAAGTGGAATGAGCAATTTGACAGAAAGTACACAAGGGGAATGCTCTTTAAAAC
  CAACGAGGAAAAAAAGACAGTGCAGATGATGTTTAAGGAAGCTAAGTTTAAAATGGGGTATGCGGATGAG
  GTACACACCCAGGTCCTGGAGCTGCCCTATGTGGAAGAGGAGCTGAGCATGGTCATTCTGCTTCCCGATG
  ACAACACGGACCTCGCCGTGGTGGAAAAAGCACTTACATATGAGAAATTCAAAGCCTGGACAAATTCAGA
  AAAGTTGACAAAAAGTAAGGTTCAAGTTTTCCTTCCCAGATTAAAGCTGGAGGAGAGTTATGACTTGGAG
  CCTTTCCTTCGAAGATTAGGAATGATCGATGCTTTTGACGAAGCCAAGGCAGACTTTTCTGGAATGTCAA
  CTGAGAAGAATGTGCCTCTGTCCAAGGTTGCCCACAAGTGCTTCGTGGAGGTCAATGAGGAAGGCACAGA
  GGCTGCCGCAGCCACTGCTGTGGTCAGGAATTCCCGGTGCAGCAGAATGGAGCCAAGATTCTGTGCAGAC
  CACCCTTTTCTTTTCTTCATCAGGCACCACAAAACCAACTGCATCTTGTTCTGTGGCAGGTTCTCTTCTC
  CG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212081 representing NM_198833
  Red=Cloning site Green=Tags(s)
  
  MDDLCEANGTFAISLFKILGEEDNSRNVFFSPMSISSALAMVFMGAKGSTAAQMSQALCLYKDGDIHRGF
  QSLLSEVNRTGTQYLLRTANRLFGEKTCDFLPDFKEYCQKFYQAELEELSFAEDTEECRKHINDWVAEKT
  EGKISEVLDAGTVDPLTKLVLVNAIYFKGKWNEQFDRKYTRGMLFKTNEEKKTVQMMFKEAKFKMGYADE
  VHTQVLELPYVEEELSMVILLPDDNTDLAVVEKALTYEKFKAWTNSEKLTKSKVQVFLPRLKLEESYDLE
  PFLRRLGMIDAFDEAKADFSGMSTEKNVPLSKVAHKCFVEVNEEGTEAAAATAVVRNSRCSRMEPRFCAD
  HPFLFFIRHHKTNCILFCGRFSSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198833
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198833.1, NP_942130.1
• RefSeq Size: 3407 bp
• RefSeq ORF: 1125 bp
• Locus ID: 5271
• Cytogenetics: 18q22.1
• Protein Families: Druggable Genome
• MW: 42.6 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the ov-serpin family of serine protease inhibitors. The encoded protein is produced by platelets and can bind to and inhibit the function of furin, a serine protease involved in platelet functions. In addition, this protein has been found to enhance the mechanical stability of cell-cell adhesion in the skin, and defects in this gene have been associated with an autosomal-recessive form of exfoliative ichthyosis. [provided by RefSeq, Jan 2017]'