POF1B (NM_024921) Human Tagged ORF Clone

CAT#: RC212627

POF1B (Myc-DDK-tagged)-Human premature ovarian failure, 1B (POF1B)

Product Images

Western blot validation of overexpression lysate (Cat# LY403038) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212627 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified POF1B protein (Cat# TP312627). The protein was produced from HEK293T cells transfected with POF1B cDNA clone (Cat# RC212627) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POF1B
• Synonyms: POF; POF2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212627 representing NM_024921
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCGAGCTATTGGAGTGAGACGAGCAGCAGCAGCTGTGGAACCCAGCAGCTCCCAGAGGTGCTGC
  AGTGCCAGCCCCAGCATTACCACTGCTACCATCAGTCAAGCCAAGCCCAGCAGCCTCCAGAAAAAAATGT
  AGTGTATGAGCGAGTGAGGACCTACAGTGGGCCCATGAACAAGGTGGTGCAGGCCTTGGACCCCTTCAAC
  TCACGGGAAGTGCTCTCCCCTCTCAAAACCACCTCCTCCTACCAAAATTTGGTTTGGAGCGACCATTCTC
  AGGAACTCCATTCTCCAACTTTAAAAATATCTACATGTGCCCCAAGTACTCTACATATAACCCAAAATAC
  TGAACAGGAACTTCATTCTCCAACAGTGAAACTTACTACATATCCACAGACCACTATTAGGAAATATGTA
  GTACAAAATCCTGAACAGGAACCACTGTCTCAATTCCTAAGAGGAAGCCATTTCTTCCCAGGAAACAATG
  TTATTTATGAAAAAACAATAAGAAAAGTGGAGAAGCTAAATACTGATCAGGGGTGCCATCCTCAGGCTCA
  ATGCCATCATCACATTATCCAACAGCCCCAGGTCATCCACTCTGCACACTGGCAACAACCTGATTCTAGC
  CAGCAAATCCAAGCCATCACAGGAAATAATCCAATTTCTACACATATTGGAAATGAACTGTGCCATAGTG
  GATCAAGCCAGATTTGTGAGCAGGTGATAATTCAGGATGATGGCCCTGAAAAATTGGACCCCAGATATTT
  TGGAGAGTTGCTTGCTGATCTGAGCCGTAAGAATACGGATCTATATCACTGCTTATTAGAACATTTGCAG
  AGAATTGGAGGAAGCAAACAGGACTTTGAGTCTACAGATGAGTCGGAAGACATTGAATCATTGATTCCTA
  AAGGATTATCAGAGTTTACAAAACAGCAAATACGCTACATTCTGCAGATGAGGGGTATGTCTGATAAGTC
  ACTCCGACTAGTGCTGTCCACATTTAGCAACATACGGGAGGAGCTTGGACATCTTCAAAATGATTTGACA
  TCACTGGAAAATGACAAGATGAGACTTGAGAAAGATTTATCATTCAAAGACACTCAATTAAAAGAGTACG
  AAGAACTCTTGGCATCAGTGAGAGCAAATAATCACCAGCAGCAGCAAGGACTTCAAGACTCAAGTTCAAA
  ATGCCAGGCATTGGAAGAAAACAATCTCTCTCTTCGACATACACTATCAGACATGGAATACAGACTAAAA
  GAACTGGAATATTGTAAACGTAATTTAGAGCAAGAGAATCAAAACCTTAGAATGCAGGTTTCTGAGACTT
  GCACAGGCCCAATGTTGCAGGCTAAAATGGATGAGATTGGCAACCACTACACGGAGATGGTAAAAAACTT
  GAGAATGGAGAAAGATAGAGAGATCTGCAGACTGAGGTCCCAATTAAACCAGTACCATAAAGATGTTTCA
  AAGAGAGAAGGAAGTTGTAGTGACTTCCAATTTAAGCTTCATGAACTGACAAGCTTGCTGGAAGAGAAGG
  ATTCCCTCATAAAGCGTCAGTCAGAGGAACTCTCCAAGTTGCGGCAAGAAATATATTCCTCTCATAACCA
  ACCCTCCACTGGTGGAAGGACTACTATTACCACTAAAAAGTACAGGACACAATATCCAATCCTAGGCCTC
  CTATATGATGACTACGAATATATACCACCAGGTAGTGAAACACAGACTATTGTGATTGAGAAAACAGAAG
  ACAAATACACTTGTCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212627 representing NM_024921
  Red=Cloning site Green=Tags(s)
  
  MSSSYWSETSSSSCGTQQLPEVLQCQPQHYHCYHQSSQAQQPPEKNVVYERVRTYSGPMNKVVQALDPFN
  SREVLSPLKTTSSYQNLVWSDHSQELHSPTLKISTCAPSTLHITQNTEQELHSPTVKLTTYPQTTIRKYV
  VQNPEQEPLSQFLRGSHFFPGNNVIYEKTIRKVEKLNTDQGCHPQAQCHHHIIQQPQVIHSAHWQQPDSS
  QQIQAITGNNPISTHIGNELCHSGSSQICEQVIIQDDGPEKLDPRYFGELLADLSRKNTDLYHCLLEHLQ
  RIGGSKQDFESTDESEDIESLIPKGLSEFTKQQIRYILQMRGMSDKSLRLVLSTFSNIREELGHLQNDLT
  SLENDKMRLEKDLSFKDTQLKEYEELLASVRANNHQQQQGLQDSSSKCQALEENNLSLRHTLSDMEYRLK
  ELEYCKRNLEQENQNLRMQVSETCTGPMLQAKMDEIGNHYTEMVKNLRMEKDREICRLRSQLNQYHKDVS
  KREGSCSDFQFKLHELTSLLEEKDSLIKRQSEELSKLRQEIYSSHNQPSTGGRTTITTKKYRTQYPILGL
  LYDDYEYIPPGSETQTIVIEKTEDKYTCP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024921
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024921.2, NM_024921.3, NP_079197.2
• RefSeq Size: 3942 bp
• RefSeq ORF: 1770 bp
• Locus ID: 79983
• MW: 67.9 kDa
• Gene Summary: Premature ovarian failure (POF) is characterized by primary or secondary amenorrhea in women less than 40 years old. Two POF susceptibility regions called "POF1" and "POF2" have been identified by breakpoint mapping of X-autosome translocations. POF1 extends from Xq21-qter while POF2 extends from Xq13.3 to Xq21.1. This gene, POF1B, resides in the POF2 region. This gene is expressed at trace levels in mouse prenatal ovary and is barely detectable or absent from adult ovary, in human and in the mouse respectively. This gene's expression is restricted to epithelia with its highest expression in the epidermis, and oro-pharyngeal and gastro-intestinal tracts. The protein encoded by this gene binds non-muscle actin filaments. The role this gene may play in the etiology of premature ovarian failure remains to be determined. [provided by RefSeq, Jan 2010]