CEP104 (NM_014704) Human Tagged ORF Clone

CAT#: RC212710

CEP104 (Myc-DDK-tagged)-Human centrosomal protein 104kDa (CEP104)

Product Images

Western blot validation of overexpression lysate (Cat# LY415082) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212710 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CEP104 protein (Cat# TP312710). The protein was produced from HEK293T cells transfected with CEP104 cDNA clone (Cat# RC212710) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CEP104
• Synonyms: CFAP256; GlyBP; JBTS25; KIAA0562; ROC22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212710 representing NM_014704
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCACAAGATTGGATTTGTAGTCGTCAGCTCATCTGGACACGAAGACGGCTTCAGTGCCCGGGAGC
  TCATGATCCACGCGCCAACTGTCAGTGGGTGGCGGTCACCTAGATTTTGCCAGTTTCCACAAGAAATTGT
  CCTTCAAATGGTGGAGAGATGTCGAATAAGGAAACTGCAGTTACTTGCTCACCAGTATATGATTTCAAGT
  AAAATTGAGTTCTACATTAGTGAAAGCTTGCCTGAATATTTTGCACCCTATCAAGCAGAGCGGTTTCGAA
  GACTTGGCTACGTGTCTCTCTGTGATAATGAAAAGACAGGTTGCAAAGCCCGGGAACTAAAATCAGTTTA
  TGTGGATGCAGTAGGACAATTTCTTAAACTGATTTTTCACCAAAACCATGTCAACAAATACAACATATAT
  AATCAGGTTGCTTTGGTTGCCATAAATATCATTGGAGACCCTGCAGATTTCAGTGATGAAAGCAATACTG
  CCTCTCGAGAGAAGTTGATTGACCACTACCTTGGGCACAACAGCGAGGACCCTGCTCTAGAAGGAACGTA
  CGCCAGGAAATCTGACTATATCTCTCCGCTAGATGACTTAGCTTTTGATATGTACCAAGATCCAGAAGTT
  GCACAGATCATACGAAAATTAGATGAAAGAAAACGGGAAGCTGTCCAAAAGGAACGCTATGATTATGCCA
  AGAAACTAAAACAAGCCATTGCTGATTTGCAAAAGGTTGGTGAGCGCCTTGGGAGGTATGAGGTAGAGAA
  ACGCTGTGCCGTGGAGAAGGAAGACTACGATCTCGCCAAGGAGAAGAAGCAGCAGATGGAGCAGTATCGT
  GCCGAGGTGTACGAGCAGCTGGAGCTGCACAGCCTCCTGGATGCCGAGCTGATGCGAAGACCTTTTGATT
  TGCCCCTCCAGCCCCTCGCTCGTTCTGGCAGTCCTTGCCACCAAAAGCCAATGCCCTCACTACCACAACT
  GGAAGAAAGAGGAACAGAAAACCAGTTTGCAGAACCTTTCCTTCAGGAAAAGCCTTCATCATATTCTCTA
  ACTATTTCTCCTCAGCATTCTGCAGTAGACCCGTTACTCCCTGCCACAGATCCTCATCCAAAGATCAATG
  CAGAGTCCCTGCCCTACGATGAGCGGCCTCTTCCAGCTATTCGTAAGCATTATGGGGAGGCAGTGGTGGA
  GCCGGAAATGAGTAATGCAGACATCAGCGATGCTCGGAGGGGAGGCATGTTAGGGGAGCCAGAGCCCTTA
  ACCGAGAAGGCCTTGAGAGAAGCCAGCTCTGCCATCGATGTGTTGGGAGAAACCTTGGTTGCTGAGGCCT
  ATTGTAAGACGTGGTCCTACCGAGAGGATGCACTGCTTGCCTTGTCTAAGAAGTTAATGGAAATGCCTGT
  TGGAACCCCAAAAGAAGATTTAAAGAACACACTGAGAGCATCCGTCTTTCTCGTTAGAAGAGCCATAAAG
  GACATTGTGACCTCCGTTTTTCAGGCTTCTTTGAAATTGTTGAAAATGATCATTACACAATATATTCCTA
  AACATAAACTGAGTAAACTTGAAACAGCTCACTGTGTGGAGAGGACCATTCCCGTTTTGCTCACCAGAAC
  TGGAGATTCTTCTGCCCGCCTCCGCGTCACAGCTGCAAATTTTATTCAGGAAATGGCCTTGTTTAAAGAA
  GTTAAGTCTCTCCAAATTATTCCATCCTACCTGGTGCAGCCATTGAAAGCAAACTCTTCAGTTCACCTGG
  CAATGAGTCAGATGGGCCTCCTGGCCCGGCTGCTGAAAGACCTGGGCACTGGCAGCTCGGGCTTCACCAT
  TGACAACGTGATGAAGTTTTCAGTGAGTGCCCTGGAGCATAGAGTGTATGAGGTCCGCGAGACGGCGGTT
  CGAATTATTTTGGACATGTACAGACAGCACCAGGCTTCCATCCTGGAGTACCTTCCTCCAGACGACAGCA
  ACACACGCAGGAACATTCTCTACAAAACAATTTTTGAGGGATTTGCTAAAATAGATGGCAGAGCTACAGA
  TGCTGAGATGAGGGCACGGAGAAAAGCGGCTACAGAAGAAGCAGAAAAACAAAAGAAAGAAGAAATAAAA
  GCTTTACAAGGGCAGCTGGCAGCACTGAAAGAAATTCAGGCTGAAGTTCAGGAAAAAGAAAGTGATGCTG
  TGAAGCCAAAGAATCAGGACATTCAAGGAGGGAAAGCAGCCCCTGCTGAAGCTCTGGGAATCCCGGATGA
  GCACTATCTAGATAATTTGTGTATTTTTTGTGGGGAAAGGAGTGAATCCTTCACAGAGGAAGGTCTGGAT
  CTCCACTACTGGAAGCACTGTCTCATGCTGACAAGATGTGACCACTGCAAACAGGTGGTCGAGATATCCA
  GTCTGACGGAGCACTTGCTGACGGAATGTGACAAAAAAGACGGGTTTGGAAAGTGTTACCGTTGCAGTGA
  GGCTGTTTTCAAGGAAGAGCTGCCCAGACACATAAAACACAAGGATTGCAACCCTGCCAAACCGGAGAAG
  CTGGCAAACCGGTGTCCCCTGTGTCATGAGAACTTCAGCCCTGGAGAAGAGGCATGGAAAGCTCACCTGA
  TGGGCCCAGCCGGCTGCACGATGAACCTGCGCAAGACACACATTCTGCAGAAGGCCCCGGCACTGCAGCC
  AGGGAAAAGCTCAGCTGTGGCCGCATCAGGCCCCTTGGGGTCAAAGGCCGGAAGCAAGATCCCCACCCCG
  AAGGGCGGACTGAGCAAGAGCTCCAGCAGGACGTACGCCAAGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212710 representing NM_014704
  Red=Cloning site Green=Tags(s)
  
  MPHKIGFVVVSSSGHEDGFSARELMIHAPTVSGWRSPRFCQFPQEIVLQMVERCRIRKLQLLAHQYMISS
  KIEFYISESLPEYFAPYQAERFRRLGYVSLCDNEKTGCKARELKSVYVDAVGQFLKLIFHQNHVNKYNIY
  NQVALVAINIIGDPADFSDESNTASREKLIDHYLGHNSEDPALEGTYARKSDYISPLDDLAFDMYQDPEV
  AQIIRKLDERKREAVQKERYDYAKKLKQAIADLQKVGERLGRYEVEKRCAVEKEDYDLAKEKKQQMEQYR
  AEVYEQLELHSLLDAELMRRPFDLPLQPLARSGSPCHQKPMPSLPQLEERGTENQFAEPFLQEKPSSYSL
  TISPQHSAVDPLLPATDPHPKINAESLPYDERPLPAIRKHYGEAVVEPEMSNADISDARRGGMLGEPEPL
  TEKALREASSAIDVLGETLVAEAYCKTWSYREDALLALSKKLMEMPVGTPKEDLKNTLRASVFLVRRAIK
  DIVTSVFQASLKLLKMIITQYIPKHKLSKLETAHCVERTIPVLLTRTGDSSARLRVTAANFIQEMALFKE
  VKSLQIIPSYLVQPLKANSSVHLAMSQMGLLARLLKDLGTGSSGFTIDNVMKFSVSALEHRVYEVRETAV
  RIILDMYRQHQASILEYLPPDDSNTRRNILYKTIFEGFAKIDGRATDAEMRARRKAATEEAEKQKKEEIK
  ALQGQLAALKEIQAEVQEKESDAVKPKNQDIQGGKAAPAEALGIPDEHYLDNLCIFCGERSESFTEEGLD
  LHYWKHCLMLTRCDHCKQVVEISSLTEHLLTECDKKDGFGKCYRCSEAVFKEELPRHIKHKDCNPAKPEK
  LANRCPLCHENFSPGEEAWKAHLMGPAGCTMNLRKTHILQKAPALQPGKSSAVAASGPLGSKAGSKIPTP
  KGGLSKSSSRTYAKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014704
• ORF Size: 2775 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014704.1, NM_014704.2, NM_014704.3, NP_055519.1
• RefSeq Size: 5848 bp
• RefSeq ORF: 2778 bp
• Locus ID: 9731
• MW: 104.3 kDa
• Gene Summary: This gene encodes a centrosomal protein required for ciliogenesis and for ciliary tip structural integrity. The mammalian protein contains three amino-terminal hydrophobic domains, two glycosylation sites, four cysteine-rich motifs, and two regions with homology to the glutamate receptor ionotropic, NMDA 1 protein. During ciliogenesis, the encoded protein translocates from the distal tips of the centrioles to the tip of the elongating cilium. Knockdown of the protein in human retinal pigment cells results in severe defects in ciliogenesis with structural deformities at the ciliary tips. Allelic variants of this gene are associated with the autosomal-recessive disorder Joubert syndrome, which is characterized by a distinctive mid-hindbrain and cerebellar malformation, oculomotor apraxia, irregular breathing, developmental delay, and ataxia. [provided by RefSeq, Feb 2016]