CCNL2 (NM_030937) Human Tagged ORF Clone

CAT#: RC213824

CCNL2 (Myc-DDK-tagged)-Human cyclin L2 (CCNL2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY410653) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213824 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCNL2
• Synonyms: ANIA-6B; CCNM; CCNS; HCLA-ISO; HLA-ISO; PCEE; SB138
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213824 representing NM_030937
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGCGGCGGCTGGTGCTGCAGGGTCGGCAGCTCCCGCGGCAGCGGCCGGCGCCCCGG
  GATCTGGGGGCGCACCCTCAGGGTCGCAGGGGGTGCTGATCGGGGACAGGCTGTACTCCGGGGTGCTCAT
  CACCTTGGAGAACTGCCTCCTGCCTGACGACAAGCTCCGTTTCACGCCGTCCATGTCGAGCGGCCTCGAC
  ACCGACACAGAGACCGACCTCCGCGTGGTGGGCTGCGAGCTCATCCAGGCGGCCGGTATCCTGCTCCGCC
  TGCCGCAGGTGGCCATGGCTACCGGGCAGGTGTTGTTCCAGCGGTTCTTTTATACCAAGTCCTTCGTGAA
  GCACTCCATGGAGCATGTGTCAATGGCCTGTGTCCACCTGGCTTCCAAGATAGAAGAGGCCCCAAGACGC
  ATACGGGACGTCATCAATGTGTTTCACCGCCTTCGACAGCTGAGAGACAAAAAGAAGCCCGTGCCTCTAC
  TACTGGATCAAGATTATGTTAATTTAAAGAACCAAATTATAAAGGCGGAAAGACGAGTTCTCAAAGAGTT
  GGGTTTCTGCGTCCATGTGAAGCATCCTCATAAGATAATCGTTATGTACCTTCAGGTGTTAGAGTGTGAG
  CGTAACCAACACCTGGTCCAGACCTCATGGAATTACATGAACGACAGCCTTCGCACCGACGTCTTCGTGC
  GGTTCCAGCCAGAGAGCATCGCCTGTGCCTGCATTTATCTTGCTGCCCGGACGCTGGAGATCCCTTTGCC
  CAATCGTCCCCATTGGTTTCTTTTGTTTGGAGCAACTGAAGAAGAAATTCAGGAAATCTGCTTAAAGATC
  TTGCAGCTTTATGCTCGGAAAAAGGTTGATCTCACACACCTGGAGGGTGAAGTGGAAAAAAGAAAGCACG
  CTATCGAAGAGGCAAAGGCCCAAGCCCGGGGCCTGTTGCCTGGGGGCACACAGGTGCTGGATGGTACCTC
  GGGGTTCTCTCCTGCCCCCAAGCTGGTGGAATCCCCCAAAGAAGGTAAAGGGAGCAAGCCTTCCCCACTG
  TCTGTGAAGAACACCAAGAGGAGGCTGGAGGGCGCCAAGAAAGCCAAGGCGGACAGCCCCGTGAACGGCT
  TGCCAAAGGGGCGAGAGAGTCGGAGTCGGAGCCGGAGCCGTGAGCAGAGCTACTCGAGGTCCCCATCCCG
  ATCAGCGTCTCCTAAGAGGAGGAAAAGTGACAGCGGCTCCACATCTGGTGGGTCCAAGTCGCAGAGCCGC
  TCCCGGAGCAGGAGTGACTCCCCACCGAGACAGGCCCCCCGCAGCGCTCCCTACAAAGGCTCTGAGATTC
  GGGGCTCCCGGAAGTCCAAGGACTGCAAGTACCCCCAGAAGCCACACAAGTCTCGGAGCCGGAGTTCTTC
  CCGTTCTCGAAGCAGGTCACGGGAGCGGGCGGATAATCCGGGAAAATACAAGAAGAAAAGTCATTACTAC
  AGAGATCAGCGACGAGAGCGCTCGAGGTCGTATGAACGCACAGGCCGTCGCTATGAGCGGGACCACCCTG
  GGCACAGCAGGCATCGGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213824 representing NM_030937
  Red=Cloning site Green=Tags(s)
  
  MAAAAAAAGAAGSAAPAAAAGAPGSGGAPSGSQGVLIGDRLYSGVLITLENCLLPDDKLRFTPSMSSGLD
  TDTETDLRVVGCELIQAAGILLRLPQVAMATGQVLFQRFFYTKSFVKHSMEHVSMACVHLASKIEEAPRR
  IRDVINVFHRLRQLRDKKKPVPLLLDQDYVNLKNQIIKAERRVLKELGFCVHVKHPHKIIVMYLQVLECE
  RNQHLVQTSWNYMNDSLRTDVFVRFQPESIACACIYLAARTLEIPLPNRPHWFLLFGATEEEIQEICLKI
  LQLYARKKVDLTHLEGEVEKRKHAIEEAKAQARGLLPGGTQVLDGTSGFSPAPKLVESPKEGKGSKPSPL
  SVKNTKRRLEGAKKAKADSPVNGLPKGRESRSRSRSREQSYSRSPSRSASPKRRKSDSGSTSGGSKSQSR
  SRSRSDSPPRQAPRSAPYKGSEIRGSRKSKDCKYPQKPHKSRSRSSSRSRSRSRERADNPGKYKKKSHYY
  RDQRRERSRSYERTGRRYERDHPGHSRHRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030937
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_030937.3, NM_030937.4, NP_112199.2
• RefSeq Size: 3107
• RefSeq ORF: 1563
• Locus ID: 81669
• Protein Families: Druggable Genome
• MW: 58 kDa
• Gene Summary: The protein encoded by this gene belongs to the cyclin family. Through its interaction with several proteins, such as RNA polymerase II, splicing factors, and cyclin-dependent kinases, this protein functions as a regulator of the pre-mRNA splicing process, as well as in inducing apoptosis by modulating the expression of apoptotic and antiapoptotic proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]