COPA (NM_001098398) Human Tagged ORF Clone

CAT#: RC214199

COPA (Myc-DDK-tagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY420566) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214199 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: COPA
• Synonyms: AILJK; alpha-COP; HEP-COP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214199 representing NM_001098398
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAACCAAATTCGAGACCAAGAGCGCGCGGGTCAAAGGGCTCAGCTTTCACCCCAAAAGACCTTGGA
  TCCTGACTAGTTTACATAATGGGGTCATCCAGTTATGGGACTATCGGATGTGCACTCTCATTGACAAGTT
  TGATGAACATGATGGTCCAGTGCGAGGCATTGACTTCCATAAGCAGCAGCCACTGTTCGTCTCTGGAGGA
  GATGACTATAAGATTAAGGTTTGGAATTACAAGCTTCGGCGCTGTCTTTTCACATTGCTTGGGCACTTAG
  ATTATATTCGCACCACGTTTTTTCATCATGAATATCCCTGGATTCTGAGTGCCTCCGATGATCAGACCAT
  CCGAGTGTGGAATTGGCAATCTAGAACCTGTGTTTGTGTGTTAACAGGGCACAACCATTATGTGATGTGT
  GCTCAGTTCCACCCCACAGAAGACTTGGTAGTATCAGCCAGCCTGGACCAGACTGTGCGCGTTTGGGATA
  TTTCTGGTCTGAGGAAAAAAAACCTGTCCCCTGGTGCGGTGGAATCGGATGTGAGAGGAATAACTGGGGT
  TGATCTATTTGGAACTACAGATGCAGTGGTGAAGCATGTACTAGAGGGTCACGATCGTGGAGTAAACTGG
  GCTGCCTTCCACCCCACTATGCCCCTTATTGTATCTGGGGCAGATGATCGTCAAGTGAAGATCTGGCGCA
  TGAATGAATCAAAGGCATGGGAGGTTGATACCTGCCGGGGCCATTACAACAATGTATCTTGTGCCGTCTT
  CCACCCTCGCCAAGAGTTGATCCTCAGCAATTCTGAGGACAAGAGTATTCGAGTCTGGGATATGTCTAAG
  CGGACTGGGGTTCAGACTTTCCGCAGAGACCATGATCGTTTCTGGGTCCTAGCTGCTCACCCTAACCTTA
  ACCTCTTTGCAGCAGGCCATGATGGTGGTATGATTGTGTTTAAGCTGGAACGGGAACGGCCAGCCTATGC
  TGTTCATGGCAATATGCTACACTATGTCAAGGACCGATTCTTACGACAGCTGGATTTCAACAGCTCCAAA
  GATGTAGCTGTGATGCAGTTGCGGAGTGGTTCCAAGTTTCCAGTATTCAATATGTCATACAATCCAGCAG
  AAAATGCAGTCCTGCTTTGTACAAGAGCTAGCAATCTAGAGAATAGTACCTATGACCTGTACACCATCCC
  TAAAGATGCTGACTCCCAGAATCCTGATGCGCCTGAAGGGAAACGATCCTCAGGCCTGACAGCCGTTTGG
  GTCGCTCGAAATCGGTTTGCTGTCCTAGATCGGATGCATTCGCTTCTGATCAAGAATCTGAAGAATGAGA
  TCACCAAAAAGGTACAGGTGCCCAACTGTGATGAGATCTTCTATGCTGGCACAGGCAATCTCCTGCTTCG
  AGATGCGGACTCTATCACACTCTTTGACGTACAGCAGAAGCGGACTCTGGCATCTGTGAAGATTTCTAAA
  GTGAAATACGTTATCTGGTCAGCAGACATGTCACATGTAGCACTACTAGCCAAACACGAACACTCATGCC
  CTTTGCCTCTTACAGCCATTGTGATCTGTAACCGCAAACTGGATGCTTTATGTAACATTCATGAGAACAT
  TCGTGTCAAGAGTGGGGCCTGGGATGAGAGTGGGGTATTTATCTATACCACAAGCAACCACATCAAATAT
  GCTGTCACCACTGGGGACCACGGGATCATTCGAACTCTGGATTTACCCATCTATGTCACACGGGTGAAGG
  GCAACAATGTATACTGCCTAGACAGGGAGTGTCGTCCCCGGGTACTCACCATTGATCCCACTGAGTTCAA
  ATTCAAGCTGGCCCTGATCAACAGAAAATATGATGAGGTACTGCACATGGTGAGGAATGCCAAACTAGTT
  GGCCAGTCTATTATTGCTTATCTCCAGAAGAAGGGCTATCCTGAAGTGGCACTGCATTTTGTCAAGGATG
  AGAAAACTCGCTTTAGTCTGGCACTGGAGTGTGGAAACATTGAGATTGCTCTGGAAGCAGCCAAAGCACT
  GGATGACAAGAACTGCTGGGAAAAGCTGGGAGAAGTGGCCCTGCTGCAGGGGAACCACCAGATTGTGGAA
  ATGTGCTATCAGCGTACCAAAAACTTTGACAAACTTTCCTTCCTGTATCTTATCACTGGCAACTTAGAAA
  AACTTCGCAAGATGATGAAGATTGCTGAGATCAGAAAGGACATGAGTGGCCACTATCAGAATGCCCTATA
  CCTGGGTGATGTGTCAGAGCGTGTGCGGATCCTGAAGAACTGTGGACAGAAGTCCCTGGCCTATCTCACA
  GCTGCTACCCATGGCTTAGATGAAGAAGCTGAGAGCCTAAAGGAGACATTTGACCCAGAGAAGGAGACAA
  TCCCAGACATTGACCCTAATGCCAAGCTGCTCCAGCCACCTGCACCTATCATGCCATTGGATACCAATTG
  GCCTTTATTGACTGTATCCAAAGGATTTTTTGAAGGCACCATTGCCAGCAAAGGGAAGGGAGGAGCACTG
  GCTGCTGACATTGACATTGACACTGTTGGTACAGAGGGCTGGGGAGAGGATGCAGAGCTGCAGTTGGATG
  AAGATGGGTTTGTGGAGGCTACAGAAGGTTTGGGGGATGATGCTCTTGGCAAGGGACAGGAAGAAGGAGG
  TGGCTGGGATGTAGAAGAAGATCTGGAGCTCCCTCCTGAGCTGGATATATCCCCTGGGGCAGCTGGTGGG
  GCTGAAGATGGTTTCTTTGTGCCCCCAACCAAGGGAACAAGTCCAACTCAGATCTGGTGTAATAACTCTC
  AGCTTCCAGTTGATCACATCCTGGCAGGCTCTTTCGAAACAGCCATGCGGCTCCTTCATGACCAAGTAGG
  GGTAATCCAGTTTGGCCCCTACAAGCAACTGTTCCTACAGACATACGCCCGAGGCCGCACAACCTATCAG
  GCTCTGCCCTGCCTACCCTCCATGTATGGCTATCCTAATCGCAACTGGAAGGATGCAGGGCTGAAGAATG
  GTGTACCAGCTGTGGGCCTGAAGCTTAATGACCTCATCCAACGGTTGCAGCTGTGCTACCAGCTCACCAC
  AGTTGGCAAATTTGAGGAGGCTGTGGAAAAATTCCGTTCCATCCTTCTCAGTGTGCCACTTCTTGTTGTG
  GACAATAAACAAGAGATTGCAGAGGCCCAGCAGCTCATCACCATTTGCCGTGAGTACATTGTGGGTTTGT
  CCGTGGAGACAGAAAGGAAGAAGCTGCCCAAAGAGACTCTAGAACAGCAGAAGCGCATCTGTGAGATGGC
  AGCCTATTTCACCCACTCAAACCTGCAGCCTGTGCACATGATCCTGGTGCTGCGTACAGCCCTCAATCTG
  TTCTTCAAGCTCAAGAACTTCAAGACAGCTGCCACCTTTGCTCGGCGCCTACTAGAACTCGGGCCCAAGC
  CTGAGGTGGCCCAACAGACCCGAAAAATCCTGTCTGCCTGTGAGAAGAATCCCACAGATGCCTACCAGCT
  CAATTATGACATGCACAACCCCTTTGACATTTGTGCTGCATCATATCGGCCCATCTACCGTGGAAAGCCA
  GTAGAAAAGTGTCCACTCAGTGGGGCCTGCTATTCCCCTGAGTTCAAAGGTCAAATCTGCAGGGTCACCA
  CAGTGACAGAGATTGGCAAAGATGTGATTGGTTTAAGGATCAGTCCTCTGCAGTTTCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214199 representing NM_001098398
  Red=Cloning site Green=Tags(s)
  
  MLTKFETKSARVKGLSFHPKRPWILTSLHNGVIQLWDYRMCTLIDKFDEHDGPVRGIDFHKQQPLFVSGG
  DDYKIKVWNYKLRRCLFTLLGHLDYIRTTFFHHEYPWILSASDDQTIRVWNWQSRTCVCVLTGHNHYVMC
  AQFHPTEDLVVSASLDQTVRVWDISGLRKKNLSPGAVESDVRGITGVDLFGTTDAVVKHVLEGHDRGVNW
  AAFHPTMPLIVSGADDRQVKIWRMNESKAWEVDTCRGHYNNVSCAVFHPRQELILSNSEDKSIRVWDMSK
  RTGVQTFRRDHDRFWVLAAHPNLNLFAAGHDGGMIVFKLERERPAYAVHGNMLHYVKDRFLRQLDFNSSK
  DVAVMQLRSGSKFPVFNMSYNPAENAVLLCTRASNLENSTYDLYTIPKDADSQNPDAPEGKRSSGLTAVW
  VARNRFAVLDRMHSLLIKNLKNEITKKVQVPNCDEIFYAGTGNLLLRDADSITLFDVQQKRTLASVKISK
  VKYVIWSADMSHVALLAKHEHSCPLPLTAIVICNRKLDALCNIHENIRVKSGAWDESGVFIYTTSNHIKY
  AVTTGDHGIIRTLDLPIYVTRVKGNNVYCLDRECRPRVLTIDPTEFKFKLALINRKYDEVLHMVRNAKLV
  GQSIIAYLQKKGYPEVALHFVKDEKTRFSLALECGNIEIALEAAKALDDKNCWEKLGEVALLQGNHQIVE
  MCYQRTKNFDKLSFLYLITGNLEKLRKMMKIAEIRKDMSGHYQNALYLGDVSERVRILKNCGQKSLAYLT
  AATHGLDEEAESLKETFDPEKETIPDIDPNAKLLQPPAPIMPLDTNWPLLTVSKGFFEGTIASKGKGGAL
  AADIDIDTVGTEGWGEDAELQLDEDGFVEATEGLGDDALGKGQEEGGGWDVEEDLELPPELDISPGAAGG
  AEDGFFVPPTKGTSPTQIWCNNSQLPVDHILAGSFETAMRLLHDQVGVIQFGPYKQLFLQTYARGRTTYQ
  ALPCLPSMYGYPNRNWKDAGLKNGVPAVGLKLNDLIQRLQLCYQLTTVGKFEEAVEKFRSILLSVPLLVV
  DNKQEIAEAQQLITICREYIVGLSVETERKKLPKETLEQQKRICEMAAYFTHSNLQPVHMILVLRTALNL
  FFKLKNFKTAATFARRLLELGPKPEVAQQTRKILSACEKNPTDAYQLNYDMHNPFDICAASYRPIYRGKP
  VEKCPLSGACYSPEFKGQICRVTTVTEIGKDVIGLRISPLQFR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098398
• ORF Size: 3699 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098398.1, NP_001091868.1
• RefSeq Size: 5666 bp
• RefSeq ORF: 3702 bp
• Locus ID: 1314
• Cytogenetics: 1q23.2
• MW: 139.1 kDa
• Gene Summary: 'In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms. [provided by RefSeq, Jul 2008]'