DAB1 (NM_021080) Human Tagged ORF Clone

CAT#: RC214664

DAB1 (Myc-DDK-tagged)-Human disabled homolog 1 (Drosophila) (DAB1)

Product Images

Western blot validation of overexpression lysate (Cat# LY402829) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214664 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DAB1 protein (Cat# TP314664). The protein was produced from HEK293T cells transfected with DAB1 cDNA clone (Cat# RC214664) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DAB1
• Synonyms: SCA37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214664 representing NM_021080
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAACTGAGACAGAACTTCAAGTAGCTGTGAAAACCAGCGCCAAGAAAGACTCCAGAAAGAAAGGTC
  AGGATCGCAGTGAAGCCACTTTGATAAAGAGGTTTAAAGGTGAAGGGGTCCGGTACAAAGCCAAATTGAT
  CGGGATTGATGAAGTTTCCGCAGCTCGGGGAGACAAGTTATGTCAAGATTCCATGATGAAACTCAAGGGC
  GTTGTTGCTGGCGCTCGTTCCAAAGGAGAACACAAACAGAAAATCTTTTTAACCATCTCCTTTGGAGGAA
  TCAAAATCTTTGATGAGAAGACAGGGGCCCTTCAGCATCATCATGCTGTTCATGAAATATCCTACATTGC
  AAAGGACATTACAGATCACCGGGCCTTTGGATATGTTTGTGGGAAGGAAGGGAATCACAGATTTGTGGCC
  ATAAAAACAGCCCAGGCGGCTGAACCTGTTATTCTGGACTTGAGAGATCTCTTTCAACTCATTTATGAAT
  TGAAGCAAAGAGAAGAATTAGAAAAAAAGGCACAAAAGGATAAGCAGTGTGAACAAGCTGTGTACCAGAC
  AATATTGGAAGAGGATGTTGAAGATCCTGTGTACCAGTACATTGTGTTTGAGGCTGGACACGAGCCAATC
  CGTGATCCCGAAACGGAAGAAAACATTTATCAGGTTCCCACCAGCCAAAAGAAGGAAGGTGTTTATGATG
  TGCCAAAAAGTCAACCTGTAAGTGCTGTGACCCAATTAGAACTTTTTGGGGACATGTCCACACCCCCTGA
  TATAACCTCTCCCCCCACTCCTGCAACTCCAGGTGATGCCTTTATCCCATCTTCATCTCAGACCCTTCCA
  GCGAGTGCAGATGTGTTTAGTTCTGTACCTTTCGGCACTGCTGCTGTACCCTCAGGTTACGTTGCAATGG
  GCGCTGTCCTCCCGTCCTTCTGGGGTCAGCAGCCCCTCGTCCAACAGCAGATGGTCATGGGTGCCCAGCC
  ACCAGTCGCTCAGGTGATGCCGGGGGCTCAGCCCATCGCATGGGGCCAGCCGGGTCTCTTTCCTGCCACT
  CAGCAGCCCTGGCCAACTGTGGCCGGGCAGTTTCCGCCAGCCGCCTTCATGCCCACACAAACTGTTATGC
  CTTTGCCAGCTGCCATGTTCCAAGGTCCCCTCACCCCCCTTGCCACCGTCCCAGGCACGAGTGACTCCAC
  CAGGTCAAGTCCACAGACCGACAAGCCCAGGCAGAAAATGGGCAAAGAAACGTTTAAGGATTTCCAGATG
  GCCCAGCCTCCGCCCGTGCCCTCCCGCAAACCCGACCAGCCCTCCCTCACCTGTACCTCAGAGGCCTTCT
  CCAGTTACTTCAACAAAGTCGGGGTGGCACAGGATACAGACGACTGTGATGACTTTGACATCTCCCAGTT
  GAATTTGACCCCTGTGACTTCTACCACACCATCGACCAACTCACCTCCAACCCCAGCCCCTAGACAGAGC
  TCTCCATCCAAATCATCTGCATCCCATGCCAGTGATCCTACCACAGATGACATCTTTGAAGAGGGCTTTG
  AAAGTCCCAGCAAAAGCGAAGAGCAAGAAGCTCCTGATGGATCACAGGCCTCATCCAACAGTGATCCATT
  TGGTGAGCCCAGTGGGGAGCCCAGTGGTGATAATATAAGTCCACAGGCCGGTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214664 representing NM_021080
  Red=Cloning site Green=Tags(s)
  
  MSTETELQVAVKTSAKKDSRKKGQDRSEATLIKRFKGEGVRYKAKLIGIDEVSAARGDKLCQDSMMKLKG
  VVAGARSKGEHKQKIFLTISFGGIKIFDEKTGALQHHHAVHEISYIAKDITDHRAFGYVCGKEGNHRFVA
  IKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVYQTILEEDVEDPVYQYIVFEAGHEPI
  RDPETEENIYQVPTSQKKEGVYDVPKSQPVSAVTQLELFGDMSTPPDITSPPTPATPGDAFIPSSSQTLP
  ASADVFSSVPFGTAAVPSGYVAMGAVLPSFWGQQPLVQQQMVMGAQPPVAQVMPGAQPIAWGQPGLFPAT
  QQPWPTVAGQFPPAAFMPTQTVMPLPAAMFQGPLTPLATVPGTSDSTRSSPQTDKPRQKMGKETFKDFQM
  AQPPPVPSRKPDQPSLTCTSEAFSSYFNKVGVAQDTDDCDDFDISQLNLTPVTSTTPSTNSPPTPAPRQS
  SPSKSSASHASDPTTDDIFEEGFESPSKSEEQEAPDGSQASSNSDPFGEPSGEPSGDNISPQAGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021080
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021080.1, NM_021080.2, NM_021080.3, NP_066566.3
• RefSeq Size: 2580 bp
• RefSeq ORF: 1668 bp
• Locus ID: 1600
• Cytogenetics: 1p32.2
• Domains: PID
• MW: 59.8 kDa
• Gene Summary: 'The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. [provided by RefSeq, Jan 2017]'