SRGAP2 (NM_001042758) Human Tagged ORF Clone

CAT#: RC215563

SRGAP2 (Myc-DDK-tagged)-Human SLIT-ROBO Rho GTPase activating protein 2 (SRGAP2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SRGAP2
• Synonyms: ARHGAP34; FNBP2; SRGAP2A; SRGAP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215563 representing NM_001042758
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGTCTCCAGCCAAATTCAAAAAGGATAAGGAGATCATAGCAGAGTACGATACTCAGGTCAAAGAGA
  TCCGTGCTCAGCTCACAGAGCAGATGAAATGCCTGGACCAGCAGTGTGAGCTTCGGGTGCAACTGTTGCA
  GGACCTCCAGGACTTCTTCCGAAAGAAGGCAGAGATTGAGATGGACTACTCCCGCAACCTGGAGAAGCTG
  GCAGAACGCTTCCTGGCCAAGACACGCAGCACCAAGGACCAGCAATTCAAGAAGGATCAGAATGTTCTCT
  CTCCAGTCAACTGCTGGAATCTCCTCTTAAACCAGGTGAAGCGGGAAAGCAGGGACCATACCACCCTGAG
  TGACATCTACCTGAATAATATCATTCCTCGATTTGTACAAGTCAGCGAGGACTCAGGAAGACTCTTTAAA
  AAGAGTAAAGAAGTCGGCCAGCAGCTCCAAGATGATTTGATGAAGGTCCTGAACGAGCTCTACTCGGTGA
  TGAAGACATATCACATGTACAATGCCGACAGCATCAGTGCTCAGAGCAAACTAAAGGAGGCGGAGAAGCA
  GGAGGAGAAGCAAATTGGTAAATCGGTAAAGCAGGAGGACCGGCAGACCCCACGCTCCCCTGACTCCACG
  GCCAACGTTCGCATTGAGGAGAAACATGTCCGGAGGAGCTCAGTGAAGAAGATTGAGAAGATGAAGGAGA
  AGCGTCAAGCCAAGTACACGGAGAATAAGCTGAAGGCCATCAAAGCCCGGAATGAGTACTTGCTGGCTTT
  GGAGGCAACCAATGCATCTGTCTTCAAGTACTACATCCATGACCTATCTGACCTTATTGATCAGTGTTGT
  GACTTAGGCTACCATGCAAGTCTGAACCGGGCTCTACGCACCTTCCTCTCTGCTGAGTTAAACCTGGAAC
  AGTCGAAGCATGAGGGTCTGGATGCCATCGAGAATGCAGTAGAAAACCTGGATGCCACCAGTGACAAGCA
  GCGCCTCATGGAGATGTACAACAACGTCTTCTGCCCCCCTATGAAGTTTGAGTTTCAGCCCCACATGGGG
  GATATGGCTTCCCAGCTCTGTGCCCAGCAGCCTGTCCAGAGTGAGCTGGTACAGAGATGCCAACAACTGC
  AGTCTCGCTTATCCACTCTAAAGATTGAAAACGAAGAGGTAAAGAAGACAATGGAGGCCACCCTGCAAAC
  CATCCAGGACATTGTGACTGTCGAGGACTTTGATGTGTCTGACTGCTTCCAGTACAGCAACTCCATGGAG
  TCCGTCAAGTCCACGGTCTCTGAAACCTTCATGAGCAAGCCCAGCATTGCTAAGAGGAGAGCCAACCAGC
  AAGAGACAGAGCAGTTTTATTTCACAAAAATGAAAGAGTACCTGGAGGGCAGGAACCTCATCACCAAGTT
  ACAAGCCAAGCATGACCTTCTGCAGAAAACCCTGGGAGAAAGTCAGCGGACAGATTGCAGTCTAGCCAGG
  CGCAGCTCAACTGTGAGGAAACAGGACTCCAGCCAGGCAATTCCTCTGGTGGTGGAAAGCTGTATCCGGT
  TTATCAGCAGACACGGACTACAGCATGAAGGAATTTTCCGGGTGTCAGGATCCCAGGTGGAAGTGAATGA
  CATCAAAAATGCCTTTGAGAGAGGAGAGGACCCCCTGGCTGGGGACCAGAACGACCATGACATGGATTCC
  ATAGCTGGTGTCCTGAAGCTTTACTTCCGGGGGCTGGAACACCCTCTCTTCCCCAAGGACATCTTTCATG
  ACCTGATGGCCTGCGTCACAATGGACAACCTGCAGGAGAGAGCTCTGCACATCCGGAAAGTCCTCCTAGT
  CCTGCCCAAAACCACTCTGATTATCATGAGATACCTCTTTGCCTTCCTCAATCATTTATCACAGTTCAGT
  GAAGAGAACATGATGGACCCCTACAACCTCGCCATCTGCTTCGGGCCCTCGCTAATGTCAGTGCCAGAGG
  GCCACGACCAGGTGTCCTGCCAAGCCCACGTGAATGAGCTGATCAAAACCATCATCATCCAGCATGAGAA
  CATCTTCCCAAGCCCCAGGGAGCTGGAGGGCCCTGTCTACAGCAGAGGAGGAAGCATGGAGGATTACTGT
  GATAGCCCTCATGGAGAGACTACCTCGGTTGAAGACTCAACCCAGGATGTGACCGCAGAGCACCACACGA
  GCGATGACGAATGTGAGCCCATCGAGGCCATTGCCAAGTTTGACTACGTGGGCCGGACAGCCCGAGAGCT
  GTCCTTTAAGAAGGGAGCATCCCTGCTGCTTTACCAGCGGGCTTCCGACGACTGGTGGGAAGGCCGGCAC
  AATGGCATCGACGGACTCATCCCCCATCAGTACATCGTGGTCCAAGACACCGAGGACGGTGTCGTGGAGA
  GGTCCAGCCCCAAGTCTGAGATTGAGGTCATTTCTGAGCCACCTGAAGAAAAGGTGACAGCCAGAGCGGG
  GGCCAGCTGTCCCAGTGGGGGTCATGTAGCCGATATTTATCTTGCAAACATCAACAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215563 representing NM_001042758
  Red=Cloning site Green=Tags(s)
  
  MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKL
  AERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFK
  KSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDST
  ANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDLIDQCC
  DLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMG
  DMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSME
  SVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLAR
  RSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDS
  IAGVLKLYFRGLEHPLFPKDIFHDLMACVTMDNLQERALHIRKVLLVLPKTTLIIMRYLFAFLNHLSQFS
  EENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPSPRELEGPVYSRGGSMEDYC
  DSPHGETTSVEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRH
  NGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVISEPPEEKVTARAGASCPSGGHVADIYLANINK
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042758
• ORF Size: 2508 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001042758.1, NP_001036223.1
• RefSeq Size: 3714
• RefSeq ORF: 2510
• Locus ID: 23380
• Protein Pathways: Axon guidance
• MW: 95.7 kDa
• Gene Summary: This locus encodes a member of the SLIT-ROBO Rho GTPase activating protein family. The encoded protein stimulates GTPase activity of Rac1, and plays a role in cortical neuron development. This locus has several paralogs on human chromosome 1 resulting from segmental duplication. While this locus itself is conserved among various species, the paralogs are found only in the genus Homo, and not in the genomes of non-human great apes. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Jul 2014]