BMP10 (NM_014482) Human Tagged ORF Clone

CAT#: RC215695

BMP10 (Myc-DDK-tagged)-Human bone morphogenetic protein 10 (BMP10)

Product Images

Western blot validation of overexpression lysate (Cat# LY415241) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215695 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BMP10
• Synonyms: MGC126783
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215695 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCTCTGGTCCTGACACTGTGCGCTCTTTTCTGCCTGGCAGCTTACTTGGTTTCTGGCAGCCCCA
  TCATGAACCTAGAGCAGTCTCCTCTGGAAGAAGATATGTCCCTCTTTGGTGATGTTTTCTCAGAGCAAGA
  CGGTGTCGACTTTAACACACTGCTCCAGAGCATGAAGGATGAGTTTCTTAAGACACTAAACCTCTCTGAC
  ATCCCCACGCAGGATTCAGCCAAGGTGGACCCACCAGAGTACATGTTGGAACTCTACAACAAATTTGCAA
  CAGATCGGACCTCCATGCCCTCTGCCAACATCATTAGGAGTTTCAAGAATGAAGATCTGTTTTCCCAGCC
  GGTCAGTTTTAATGGGCTCCGAAAATACCCCCTCCTCTTCAATGTGTCCATTCCTCACCATGAAGAGGTC
  ATCATGACTGAACTTAGGCTATACACACTGGTGCAAAGGGATCGTATGATATACGATGGAGTAGACCGGA
  AAATTACCATTTTTGAAGTGCTGGAGAGCAAAGGGGATAATGAGGGAGAAAGAAACATGCTGGTCTTGGT
  GTCTGGGGAGATATATGGAACCAACAGTGAGTGGGAGACTTTTGATGTCACAGATGCCATCAGACGTTGG
  CAAAAGTCAGGCTCATCCACCCACCAGCTGGAGGTCCACATTGAGAGCAAACACGATGAAGCTGAGGATG
  CCAGCAGTGGACGGCTAGAAATAGATACCAGTGCCCAGAATAAGCATAACCCTTTGCTCATCGTGTTTTC
  TGATGACCAAAGCAGTGACAAGGAGAGGAAGGAGGAACTGAATGAAATGATTTCCCATGAGCAACTTCCA
  GAGCTGGACAACTTGGGCCTGGATAGCTTTTCCAGTGGACCTGGGGAAGAGGCTTTGTTGCAGATGAGAT
  CAAACATCATCTATGACTCCACTGCCCGAATCAGAAGGAACGCCAAAGGAAACTACTGTAAGAGGACCCC
  GCTCTACATCGACTTCAAGGAGATTGGGTGGGACTCCTGGATCATCGCTCCGCCTGGATACGAAGCCTAT
  GAATGCCGTGGTGTTTGTAACTACCCCCTGGCAGAGCATCTCACACCCACAAAGCATGCAATTATCCAGG
  CCTTGGTCCACCTCAAGAATTCCCAGAAAGCTTCCAAAGCCTGCTGTGTGCCCACAAAGCTAGAGCCCAT
  CTCCATCCTCTATTTAGACAAAGGCGTCGTCACCTACAAGTTTAAATACGAAGGCATGGCCGTCTCCGAA
  TGTGGCTGTAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215695 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSLVLTLCALFCLAAYLVSGSPIMNLEQSPLEEDMSLFGDVFSEQDGVDFNTLLQSMKDEFLKTLNLSD
  IPTQDSAKVDPPEYMLELYNKFATDRTSMPSANIIRSFKNEDLFSQPVSFNGLRKYPLLFNVSIPHHEEV
  IMTELRLYTLVQRDRMIYDGVDRKITIFEVLESKGDNEGERNMLVLVSGEIYGTNSEWETFDVTDAIRRW
  QKSGSSTHQLEVHIESKHDEAEDASSGRLEIDTSAQNKHNPLLIVFSDDQSSDKERKEELNEMISHEQLP
  ELDNLGLDSFSSGPGEEALLQMRSNIIYDSTARIRRNAKGNYCKRTPLYIDFKEIGWDSWIIAPPGYEAY
  ECRGVCNYPLAEHLTPTKHAIIQALVHLKNSQKASKACCVPTKLEPISILYLDKGVVTYKFKYEGMAVSE
  CGCR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014482
• ORF Size: 1272 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014482.1, NM_014482.2, NP_055297.1
• RefSeq Size: 1584 bp
• RefSeq ORF: 1275 bp
• Locus ID: 27302
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• MW: 48.1 kDa
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which binds to the activin receptor-like kinase 1 (ALK1) and plays important roles in cardiovascular development including cardiomyocyte proliferation and regulation of heart size, closure of the ductus arteriosus, angiogenesis and ventricular trabeculation. [provided by RefSeq, Aug 2016]