TRAPPC9 (NM_031466) Human Tagged ORF Clone

CAT#: RC215800

TRAPPC9 (Myc-DDK-tagged)-Human trafficking protein particle complex 9 (TRAPPC9), transcript variant 1

Product Images

Coomassie blue staining of purified TRAPPC9 protein (Cat# TP315800). The protein was produced from HEK293T cells transfected with TRAPPC9 cDNA clone (Cat# RC215800) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRAPPC9
• Synonyms: IBP; IKBKBBP; MRT13; NIBP; T1; TRS120
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215800 representing NM_031466
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCAGCTGGGGATCAAGACCGCGCGCCACACAGGGGGAAGCCGGCCCAGGCTGGGGCTCGCACCT
  CACGTGCCTCCCGGGCCCTGCGATCCTGGAGGCGCTCCCAGGCCGCGCGCGCCACGGTCACCCACCCACG
  TGGGGGGCACGACCGTGGGAGTCACGGGGGGTACCGTGAGGGTCACAGGGGGTGCCGCAGGGATCCACAG
  TGGGCTTCCGCGGGGCCTCCACCCCTGAGCTTCACAGAGGAAGTGAAATTTGAGCTGCGCGCCCTGAAGG
  ACTGGGACTTCAAAATGAGCGTCCCTGACTACATGCAGTGTGCTGAGGACCACCAGACGCTGCTCGTGGT
  GGTCCAGCCTGTGGGCATCGTCTCCGAGGAGAACTTCTTCAGGATCTATAAGAGGATTTGCTCTGTGAGT
  CAGATCAGCGTGCGGGACTCCCAGCGAGTCCTCTACATCCGCTACAGGCACCACTACCCACCCGAGAACA
  ACGAGTGGGGTGACTTCCAGACCCACCGCAAAGTCGTGGGCCTCATCACCATCACAGACTGCTTCTCGGC
  CAAGGACTGGCCACAGACCTTTGAGAAGTTCCACGTGCAGAAGGAGATCTACGGCTCCACACTGTATGAC
  TCCCGGCTCTTTGTCTTCGGGCTGCAGGGGGAGATCGTGGAGCAGCCGCGCACCGACGTGGCTTTCTACC
  CCAACTACGAGGACTGCCAGACGGTGGAGAAGAGAATCGAGGACTTCATCGAGTCACTGTTCATCGTGCT
  GGAGTCCAAGCGTCTGGACAGAGCCACAGACAAGTCTGGGGATAAGATCCCCCTTCTCTGTGTCCCGTTT
  GAGAAAAAGGACTTTGTAGGACTGGACACAGACAGCAGACATTACAAGAAGCGGTGCCAAGGCCGCATGC
  GGAAGCACGTGGGGGACCTGTGCCTGCAGGCAGGGATGCTGCAGGACTCCCTGGTGCATTACCACATGTC
  GGTGGAGCTGCTGCGTTCTGTGAATGACTTTCTGTGGCTTGGAGCTGCCCTGGAAGGATTGTGTTCAGCT
  TCTGTCATCTATCACTATCCTGGTGGAACTGGTGGGAAGAGTGGAGCTCGGAGGTTCCAGGGCAGCACCC
  TTCCTGCTGAAGCAGCCAATAGACACCGGCCAGGGGCACAGGAAGTTCTCATTGATCCAGGTGCCCTCAC
  CACCAATGGCATCAACCCTGACACCAGTACTGAGATCGGACGTGCTAAGAACTGCCTTAGCCCTGAAGAC
  ATAATTGACAAGTATAAAGAGGCGATTTCCTATTACAGCAAGTATAAGAATGCGGGAGTGATTGAGTTGG
  AAGCGTGCATCAAGGCTGTACGTGTCCTTGCAATTCAGAAACGGAGCATGGAAGCATCAGAATTTCTTCA
  GAATGCAGTTTACATTAACCTTCGACAGCTTTCTGAGGAAGAGAAAATTCAGCGCTACAGCATCCTCTCC
  GAGCTCTATGAGCTGATCGGCTTCCATCGCAAGTCTGCGTTCTTCAAGCGCGTGGCCGCCATGCAGTGCG
  TGGCCCCAAGCATCGCGGAGCCTGGGTGGAGGGCCTGCTACAAACTCCTCCTGGAAACGCTGCCCGGCTA
  CAGTCTGTCGCTGGATCCCAAAGATTTCAGCAGAGGCACGCACAGAGGCTGGGCTGCGGTCCAGATGCGT
  TTGCTCCATGAATTGGTCTACGCCTCCCGAAGGATGGGGAACCCTGCCCTCTCTGTCAGACACCTGTCCT
  TCCTTCTACAGACCATGCTGGACTTCTTGTCGGATCAGGAAAAGAAAGATGTGGCCCAAAGCCTAGAGAA
  CTATACGTCCAAGTGTCCTGGGACCATGGAGCCCATCGCCCTCCCTGGCGGCCTCACCCTGCCACCGGTG
  CCCTTCACCAAGCTTCCCATCGTCAGGCATGTGAAACTATTGAACCTTCCTGCTAGCCTCCGGCCACACA
  AAATGAAAAGCTTGCTGGGTCAGAACGTGTCAACCAAAAGTCCTTTCATCTATTCACCAATTATCGCACA
  CAACCGTGGAGAAGAGCGGAACAAGAAAATAGATTTCCAGTGGGTTCAAGGAGATGTGTGTGAAGTTCAG
  CTGATGGTATATAACCCAATGCCGTTTGAACTTCGAGTTGAAAACATGGGGCTGCTCACCAGCGGAGTGG
  AGTTCGAGTCTCTCCCTGCGGCGCTTTCTCTTCCGGCTGAATCTGGTCTGTACCCAGTGACGCTCGTCGG
  GGTCCCGCAGACGACTGGAACGATTACTGTGAACGGTTACCATACCACGGTCTTCGGTGTGTTCAGTGAC
  TGTTTGCTGGATAACCTGCCGGGAATAAAAACCAGTGGCTCCACAGTGGAAGTCATTCCCGCGTTGCCAA
  GACTGCAGATCAGCACCTCTCTGCCCAGATCTGCACATTCATTGCAACCTTCTTCTGGTGATGAAATATC
  TACTAATGTATCTGTCCAGCTTTACAATGGAGAAAGTCAGCAACTAATCATTAAATTGGAAAATATTGGA
  ATGGAACCATTGGAGAAACTGGAGGTCACCTCGAAAGTTCTCACCACTAAAGAAAAATTGTATGGCGACT
  TCTTGAGCTGGAAGCTAGAGGAAACCCTTGCCCAGTTCCCTTTGCAGCCTGGGAAGGTGGCCACGTTCAC
  AATCAACATCAAAGTGAAGCTGGATTTCTCCTGCCAGGAGAATCTCCTGCAGGATCTCAGTGATGATGGA
  ATCAGTGTGAGTGGCTTTCCCCTGTCCAGTCCTTTTCGGCAGGTCGTTCGGCCCCGAGTGGAGGGCAAAC
  CTGTGAACCCACCCGAGAGCAACAAAGCAGGCGACTACAGCCACGTGAAGACCCTGGAAGCTGTCCTGAA
  TTTCAAATACTCTGGAGGCCCGGGCCACACTGAAGGATATTACAGGAATCTCTCCCTGGGGCTGCATGTA
  GAAGTCGAGCCGTCTGTATTTTTCACCCGAGTCAGCACCCTCCCAGCAACCAGTACCCGGCAGTGTCACC
  TGCTCCTGGATGTCTTCAACTCCACCGAGCATGAGCTGACCGTCAGCACCAGGAGCAGCGAGGCACTCAT
  CCTGCACGCCGGCGAGTGCCAGCGAATGGCTATTCAAGTGGACAAGTTCAACTTTGAGAGTTTCCCGGAG
  TCCCCTGGGGAGAAGGGGCAATTTGCAAACCCCAAGCAGCTGGAGGAAGAGCGGCGGGAAGCCCGAGGCC
  TGGAGATCCACAGCAAGCTGGGCATCTGCTGGAGAATCCCCTCCCTGAAGCGCAGTGGCGAGGCGAGTGT
  GGAAGGACTCCTGAACCAGCTCGTCCTGGAGCACCTGCAGCTGGCGCCTCTGCAGTGGGATGTGCTGGTG
  GACGGACAGCCATGTGACCGCGAGGCTGTGGCGGCCTGCCAGGTGGGCGACCCCGTGCGCCTGGAGGTGC
  GGCTGACCAACCGGAGCCCGCGCAGCGTAGGGCCCTTCGCCCTCACTGTGGTCCCCTTCCAGGACCACCA
  GAACGGCGTGCACAACTACGACCTGCACGACACCGTCTCCTTCGTGGGCTCCAGCACCTTCTACCTCGAC
  GCGGTGCAGCCGTCCGGCCAGTCGGCCTGCCTCGGGGCCCTCCTCTTCCTCTACACGGGAGACTTCTTCC
  TCCACATCCGGTTCCACGAGGACAGCACCAGCAAGGAGCTGCCACCCTCTTGGTTCTGCCTGCCCAGTGT
  GCACGTGTGTGCCCTGGAGGCGCAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215800 representing NM_031466
  Red=Cloning site Green=Tags(s)
  
  MVPAGDQDRAPHRGKPAQAGARTSRASRALRSWRRSQAARATVTHPRGGHDRGSHGGYREGHRGCRRDPQ
  WASAGPPPLSFTEEVKFELRALKDWDFKMSVPDYMQCAEDHQTLLVVVQPVGIVSEENFFRIYKRICSVS
  QISVRDSQRVLYIRYRHHYPPENNEWGDFQTHRKVVGLITITDCFSAKDWPQTFEKFHVQKEIYGSTLYD
  SRLFVFGLQGEIVEQPRTDVAFYPNYEDCQTVEKRIEDFIESLFIVLESKRLDRATDKSGDKIPLLCVPF
  EKKDFVGLDTDSRHYKKRCQGRMRKHVGDLCLQAGMLQDSLVHYHMSVELLRSVNDFLWLGAALEGLCSA
  SVIYHYPGGTGGKSGARRFQGSTLPAEAANRHRPGAQEVLIDPGALTTNGINPDTSTEIGRAKNCLSPED
  IIDKYKEAISYYSKYKNAGVIELEACIKAVRVLAIQKRSMEASEFLQNAVYINLRQLSEEEKIQRYSILS
  ELYELIGFHRKSAFFKRVAAMQCVAPSIAEPGWRACYKLLLETLPGYSLSLDPKDFSRGTHRGWAAVQMR
  LLHELVYASRRMGNPALSVRHLSFLLQTMLDFLSDQEKKDVAQSLENYTSKCPGTMEPIALPGGLTLPPV
  PFTKLPIVRHVKLLNLPASLRPHKMKSLLGQNVSTKSPFIYSPIIAHNRGEERNKKIDFQWVQGDVCEVQ
  LMVYNPMPFELRVENMGLLTSGVEFESLPAALSLPAESGLYPVTLVGVPQTTGTITVNGYHTTVFGVFSD
  CLLDNLPGIKTSGSTVEVIPALPRLQISTSLPRSAHSLQPSSGDEISTNVSVQLYNGESQQLIIKLENIG
  MEPLEKLEVTSKVLTTKEKLYGDFLSWKLEETLAQFPLQPGKVATFTINIKVKLDFSCQENLLQDLSDDG
  ISVSGFPLSSPFRQVVRPRVEGKPVNPPESNKAGDYSHVKTLEAVLNFKYSGGPGHTEGYYRNLSLGLHV
  EVEPSVFFTRVSTLPATSTRQCHLLLDVFNSTEHELTVSTRSSEALILHAGECQRMAIQVDKFNFESFPE
  SPGEKGQFANPKQLEEERREARGLEIHSKLGICWRIPSLKRSGEASVEGLLNQLVLEHLQLAPLQWDVLV
  DGQPCDREAVAACQVGDPVRLEVRLTNRSPRSVGPFALTVVPFQDHQNGVHNYDLHDTVSFVGSSTFYLD
  AVQPSGQSACLGALLFLYTGDFFLHIRFHEDSTSKELPPSWFCLPSVHVCALEAQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031466
• ORF Size: 3738 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031466.3, NM_031466.4, NM_031466.5, NM_031466.6, NM_031466.7, NP_113654.3
• RefSeq Size: 4473
• RefSeq ORF: 3741
• Locus ID: 83696
• MW: 139.3 kDa
• Gene Summary: This gene encodes a protein that likely plays a role in NF-kappa-B signaling. Mutations in this gene have been associated with autosomal-recessive cognitive disability. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]