AKAP6 (NM_004274) Human Tagged ORF Clone

CAT#: RC215986

AKAP6 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 6 (AKAP6)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AKAP6
• Synonyms: ADAP6; ADAP100; AKAP100; mAKAP; PRKA6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215986 representing NM_004274
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAACCATGAGCGTGACACTTTCCCCCCTGAGGTCACAGGACCTGGATCCCATGGCTACTGATGCTT
  CACCCATGGCCATCAACATGACACCCACTGTGGAGCAGGGTGAGGGAGAAGAGGCAATGAAGGACATGGA
  CTCTGACCAGCAGTATGAAAAGCCACCCCCACTACACACAGGGGCTGACTGGAAGATTGTCCTCCACTTA
  CCTGAAATTGAGACCTGGCTCCGGATGACCTCAGAGAGGGTCCGAGACCTAACCTATTCAGTCCAGCAGG
  ATTCGGACAGCAAGCATGTGGATGTACATCTAGTTCAACTAAAGGACATTTGTGAAGATATTTCTGATCA
  TGTTGAGCAAATCCATGCCCTCCTTGAAACAGAGTTCTCCCTAAAGCTGCTGTCTTACTCTGTCAACGTG
  ATAGTGGACATCCACGCAGTGCAGCTCCTCTGGCACCAGCTTCGAGTCTCAGTGCTGGTTCTGCGGGAGC
  GCATTCTGCAAGGTCTGCAGGACGCCAATGGCAACTACACTAGGCAGACGGACATTCTGCAAGCTTTCTC
  TGAAGAGACAAAAGAGGGCCGGCTTGATTCTCTAACAGAAGTGGATGACTCAGGACAATTAACCATCAAA
  TGTTCTCAAAATTACTTGTCTCTGGATTGTGGCATTACTGCATTCGAACTGTCTGACTACAGTCCAAGTG
  AGGATTTGCTCAGTGGGCTAGGTGACATGACCTCTAGCCAAGTCAAAACCAAACCCTTTGACTCTTGGAG
  CTACAGTGAGATGGAAAAGGAGTTTCCTGAGCTTATCCGAAGTGTTGGTTTACTTACGGTAGCTGCTGAC
  TCTATCTCTACCAATGGCAGTGAAGCAGTTACTGAGGAGGTATCTCAAGTATCTCTCTCAGTAGACGACA
  AAGGTGGATGTGAGGAAGACAATGCTTCTGCAGTCGAAGAGCAACCAGGCTTAACACTGGGGGTGTCATC
  ATCTTCAGGAGAAGCTCTGACAAATGCTGCTCAACCCTCCTCTGAGACTGTGCAGCAAGAATCCAGTTCC
  TCCTCCCATCATGATGCAAAGAATCAGCAGCCTGTTCCTTGTGAAAATGCAACCCCCAAACGAACCATCA
  GAGATTGCTTTAATTATAACGAGGACTCTCCCACACAGCCTACATTGCCAAAAAGAGGACTTTTTCTTAA
  AGAGGAAACTTTTAAGAATGATCTGAAAGGCAATGGTGGAAAGAGGCAAATGGTTGATCTAAAGCCTGAG
  ATGAGCAGAAGCACCCCTTCGCTAGTAGATCCTCCTGACAGATCCAAACTTTGCCTGGTATTGCAGTCTT
  CTTACCCCAACAGCCCTTCTGCTGCCAGCCAGTCTTATGAGTGTTTACACAAGGTGGGGAATGGGAACCT
  TGAAAACACAGTCAAATTTCACATTAAAGAAATTTCTTCCAGCCTGGGAAGGCTTAACGACTGCTATAAA
  GAGAAATCTCGACTTAAAAAGCCACACAAGACCTCAGAAGAGGTGCCTCCATGCCGAACACCTAAACGGG
  GGACTGGTTCAGGCAAACAAGCTAAAAATACAAAGAGCTCAGCAGTGCCAAATGGAGAGCTTTCTTATAC
  TTCCAAGGCCATAGAGGGGCCACAAACAAATTCTGCTTCCACATCCTCACTTGAGCCTTGTAATCAGAGA
  AGTTGGAATGCCAAATTGCAATTGCAGTCAGAAACATCCAGTTCACCAGCTTTTACTCAGAGCAGTGAAT
  CCTCTGTTGGCTCAGACAACATCATGTCTCCGGTGCCACTTCTTTCAAAACACAAAAGCAAAAAAGGTCA
  AGCCTCCTCTCCAAGTCACGTCACTAGGAATGGTGAGGTTGTGGAGGCCTGGTATGGCTCTGATGAATAC
  CTAGCACTGCCCTCTCACCTTAAGCAGACAGAAGTATTGGCTTTGAAGTTGGAAAACCTAACAAAGCTTC
  TGCCTCAGAAACCCAGAGGAGAAACCATCCAGAATATTGATGACTGGGAACTGTCTGAAATGAATTCAGA
  TTCTGAAATCTATCCAACCTATCATGTCAAAAAGAAGCATACAAGGCTAGGCAGGGTGTCTCCAAGCTCA
  TCTAGTGACATAGCCTCTTCACTAGGGGAGAGCATTGAATCTGGGCCCCTGAGTGACATTCTTTCTGATG
  AGGAGTCCAGTATGCCTCTCGCTGGCATGAAAAAGTATGCTGATGAGAAGTCAGAAAGAGCTTCATCCTC
  TGAGAAAAATGAGAGCCATTCTGCCACTAAATCAGCTTTAATTCAGAAACTGATGCAAGATATTCAGCAC
  CAAGACAACTATGAAGCCATATGGGAAAAAATAGAGGGGTTTGTAAACAAACTGGATGAATTCATTCAAT
  GGTTAAATGAAGCCATGGAAACTACAGAGAATTGGACTCCCCCTAAAGCAGAGATGGATGACCTTAAACT
  GTATCTGGAGACACACTTGAGTTTTAAGTTGAATGTAGACAGTCATTGTGCTCTCAAGGAAGCTGTGGAG
  GAGGAAGGACACCAACTTCTTGAGCTTATTGCATCTCACAAAGCAGGACTGAAGGACATGCTGCGGATGA
  TTGCAAGTCAATGGAAGGAGCTGCAGAGGCAAATCAAACGGCAGCACAGCTGGATTCTCAGGGCTCTGGA
  TACCATCAAAGCCGAGATACTGGCTACTGATGTGTCTGTGGAGGATGAGGAAGGGACTGGAAGCCCCAAG
  GCTGAGGTTCAACTATGCTACCTGGAAGCACAAAGAGATGCTGTTGAGCAGATGTCCCTCAAGCTGTACA
  GCGAGCAGTATACCAGCAGCAGCAAGCGAAAGGAAGAGTTTGCTGATATGTCAAAAGTTCATTCAGTGGG
  AAGCAATGGGCTTCTGGACTTTGATTCAGAATATCAGGAGCTCTGGGATTGGCTGATTGACATGGAGTCC
  CTTGTGATGGACAGCCACGACCTGATGATGTCAGAGGAGCAGCAGCAGCATCTTTACAAGCGATACAGTG
  TGGAAATGTCCATCAGACACCTGAAAAAGACGGAGCTGCTTAGTAAGGTTGAAGCTTTGAAGAAAGGTGG
  CGTTTTACTACCAAATGATCTCCTTGAAAAAGTGGATTCAATTAATGAAAAATGGGAACTGCTTGGGAAA
  ACCCTAGGAGAGAAGATCCAGGACACAATGGCAGGGCACAGTGGGTCGAGTCCACGTGACCTGCTCTCTC
  CTGAAAGTGGAAGCCTGGTAAGGCAGCTGGAGGTCAGGATCAAAGAACTGAAAGGATGGCTAAGAGATAC
  AGAGCTTTTCATCTTCAATTCCTGTCTGAGACAAGAAAAGGAAGGAACAATGAATACTGAGAAACAACTG
  CAATACTTTAAGTCCCTCTGTCGTGAAATCAAGCAACGACGTCGAGGAGTTGCCTCCATTCTGCGACTAT
  GCCAGCATCTTTTGGATGACCGGGAGACTTGCAATCTGAATGCAGATCACCAGCCCATGCAGCTGATCAT
  TGTAAATCTTGAAAGAAGGTGGGAAGCCATTGTCATGCAAGCCGTCCAGTGGCAAACACGTCTACAAAAG
  AAGATGGGAAAGGAATCTGAGACTTTGAATGTGATTGATCCTGGCTTGATGGACCTAAATGGGATGAGTG
  AGGATGCCCTGGAATGGGATGAAATGGACATAAGTAACAAGTTAATTAGTTTGAATGAGGAATCAAATGA
  CCTTGATCAAGAACTCCAACCTGTTATCCCTTCCTTGAAGCTTGGAGAGACAAGTAATGAGGACCCTGGT
  TATGACGAGGAGGCTGATAACCATGGGGGATCTCAGTATGCCTCAAATATTACTGCCCCCTCTAGTCCAC
  ACATTTACCAGGTGTACAGCCTCCACAATGTTGAACTCTATGAGGACAACCACATGCCATTTCTGAAAAA
  CAATCCAAAGGTCACTGGCATGACACAGCCTAATGTTTTAACTAAGAGTCTCAGTAAAGACTCTTCATTT
  TCATCTACCAAATCTTTGCCAGATCTTCTAGGTGGTTCCAATTTGGTAAAGCCCTGCGCATGTCATGGAG
  GAGACATGAGCCAGAATTCAGGCAGTGAGAGTGGAATTGTCAGTGAAGGAGACACAGAAACCACTACCAA
  CTCTGAAATGTGCTTGCTCAATGCAGTGGATGGGTCCCCAAGTAACCTTGAAACTGAACATCTGGACCCA
  CAAATGGGAGATGCAGTTAACGTGTTAAAGCAAAAATTTACAGATGAGGGGGAAAGCATTAAGCTTCCAA
  ATAGCTCTCAGTCGTCCATTTCACCAGTGGGTTGTGTAAATGGAAAAGTTGGAGATTTAAACAGTATTAC
  CAAACATACCCCTGACTGTTTGGGAGAAGAATTACAAGGAAAACATGATGTGTTTACATTTTATGATTAC
  TCATACCTCCAAGGCTCAAAACTCAAATTACCAATGATAATGAAACAGTCACAAAGCGAAAAAGCGCATG
  TGGAGGATCCCCTGCTTCGTGGTTTTTATTTTGATAAAAAATCATGCAAATCTAAACATCAGACTACAGA
  GTTACAACCAGATGTACCTCCCCATGAAAGGATTTTGGCAAGTGCATCTCATGAAATGGATCGCATTTCA
  TATAAAAGTGGCAATATAGAAAAGACATTCACTGGCATGCAGAATGCCAAACAGCTCTCCCTTTTATCTC
  ATAGTTCATCTATTGAGTCCCTTTCTCCAGGGGGTGATTTATTTGGATTGGGCATCTTTAAAAATGGCAG
  TGACAGCCTCCAGCGAAGCACTTCTTTAGAAAGTTGGTTGACTTCCTATAAAAGCAATGAAGATCTCTTT
  AGCTGTCACAGCTCTGGGGATATAAGCGTGAGCAGTGGCTCAGTTGGTGAACTAAGTAAAAGAACATTAG
  ATCTCCTGAATCGTTTGGAGAATATCCAGAGCCCCTCAGAGCAAAAGATAAAACGAAGTGTTTCTGATAT
  CACTCTTCAAAGCAGTTCCCAAAAGATGTCCTTTACTGGCCAGATGTCATTGGACATAGCATCTTCTATC
  AATGAAGACTCAGCGGCATCTCTAACAGAACTTAGCAGCAGTGACGAGCTCTCTCTTTGCTCAGAGGATA
  TTGTGTTACACAAGAACAAGATCCCGGAATCGAATGCATCGTTCAGGAAGCGTCTGACTCGTTCAGTGGC
  TGATGAAAGCGATGTCAATGTCAGCATGATTGTTAATGTCTCTTGCACCTCTGCTTGCACTGATGATGAA
  GATGACAGCGACCTGCTCTCCAGCTCTACCCTTACCTTGACTGAAGAAGAGCTGTGCATCAAAGATGAGG
  ATGACGACTCCAGTATTGCAACAGATGATGAAATTTATGAAGACTGCACCTTGATGTCAGGGCTAGACTA
  CATAAAGAATGAATTACAGACCTGGATTAGGCCAAAATTGTCTTTGACAAGAGATAAGAAAAGGTGCAAT
  GTCAGTGATGAGATGAAGGGCAGTAAAGATATAAGTAGCAGTGAGATGACCAATCCCTCTGATACTCTGA
  ATATTGAGACCCTTCTAAATGGCTCTGTAAAACGTGTCTCTGAAAATAATGGAAATGGTAAGAATTCATC
  TCATACCCATGAGTTAGGGACAAAGCGTGAAAATAAGAAAACTATTTTCAAAGTTAATAAAGATCCATAT
  GTGGCTGACATGGAAAATGGCAATATTGAAGGTATTCCAGAAAGGCAAAAGGGAAAACCGAATGTGACTT
  CAAAGGTATCAGAAAATCTTGGTTCACATGGGAAAGAGATTTCAGAGAGTGAGCATTGTAAGTGTAAAGC
  ACTTATGGATAGTTTAGATGATTCAAATACTGCTGGCAAGGAATTTGTTTCCCAAGATGTTAGACATCTT
  CCAAAGAAATGTCCAAATCACCACCATTTTGAAAATCAAAGCACTGCCTCTACTCCCACTGAGAAGTCTT
  TCTCAGAACTGGCTTTAGAAACCAGGTTTAACAACAGACAAGACTCTGATGCACTGAAATCATCTGATGA
  TGCACCGAGTATGGCTGGAAAATCTGCTGGTTGTTGCCTAGCACTTGAACAAAACGGAACAGAGGAAAAT
  GCTTCTATCAGCGACATTTCCTGTTGCAACTGTGAGCCAGATGTTTTCCATCAAAAAGATGCCGAAGATT
  GTTCAGTACACAACTTTGTTAAGGAAATCATTGACATGGCTTCGACAGCCCTAAAAAGTAAATCTCAACC
  TGAAAACGAGGTGGCTGCTCCTACTTCATTAACTCAAATCAAGGAGAAAGTGTTGGAGCATTCTCACCGG
  CCCATCCAGCTGAGAAAAGGGGACTTTTATTCGTACTTATCTCTCTCATCTCATGACAGTGATTGTGGGG
  AGGTCACCAATTACATAGAAGAGAAAAGCAGCACTCCATTGCCACTAGACACCACTGACTCGGGCTTAGA
  TGACAAGGAAGATATTGAATGCTTTTTTGAGGCCTGTGTTGAGGGTGACTCTGATGGAGAGGAGCCTTGT
  TACTCTAGTGCTCCTCCAAATGAATCTGCAGTTCCCAGCGAAGCTGCAATGCCACTACAAGCAACAGCAT
  GTTCTTCTGAGTTCAGTGATAGTTCTCTTTCAGCTGATGATGCAGATACAGTGGCTCTTTCAAGTCCTTC
  CTCTCAGGAAAGAGCTGAGGTTGGAAAGGAAGTGAATGGTTTGCCCCAAACTTCCAGTGGCTGTGCAGAA
  AACTTAGAGTTTACTCCTTCAAAGCTTGACAGTGAAAAGGAAAGTTCCGGAAAACCAGGTGAATCTGGAA
  TGCCAGAAGAACATAATGCTGCTTCAGCCAAATCTAAAGTTCAAGACCTCTCCTTGAAGGCAAATCAGCC
  AACAGACAAGGCCGCATTGCATCCCAGCCCCAAAACTTTAACCTGTGAAGAAAATCTTCTAAACCTTCAT
  GAAAAACGACATAGAAATATGCATAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215986 representing NM_004274
  Red=Cloning site Green=Tags(s)
  
  MLTMSVTLSPLRSQDLDPMATDASPMAINMTPTVEQGEGEEAMKDMDSDQQYEKPPPLHTGADWKIVLHL
  PEIETWLRMTSERVRDLTYSVQQDSDSKHVDVHLVQLKDICEDISDHVEQIHALLETEFSLKLLSYSVNV
  IVDIHAVQLLWHQLRVSVLVLRERILQGLQDANGNYTRQTDILQAFSEETKEGRLDSLTEVDDSGQLTIK
  CSQNYLSLDCGITAFELSDYSPSEDLLSGLGDMTSSQVKTKPFDSWSYSEMEKEFPELIRSVGLLTVAAD
  SISTNGSEAVTEEVSQVSLSVDDKGGCEEDNASAVEEQPGLTLGVSSSSGEALTNAAQPSSETVQQESSS
  SSHHDAKNQQPVPCENATPKRTIRDCFNYNEDSPTQPTLPKRGLFLKEETFKNDLKGNGGKRQMVDLKPE
  MSRSTPSLVDPPDRSKLCLVLQSSYPNSPSAASQSYECLHKVGNGNLENTVKFHIKEISSSLGRLNDCYK
  EKSRLKKPHKTSEEVPPCRTPKRGTGSGKQAKNTKSSAVPNGELSYTSKAIEGPQTNSASTSSLEPCNQR
  SWNAKLQLQSETSSSPAFTQSSESSVGSDNIMSPVPLLSKHKSKKGQASSPSHVTRNGEVVEAWYGSDEY
  LALPSHLKQTEVLALKLENLTKLLPQKPRGETIQNIDDWELSEMNSDSEIYPTYHVKKKHTRLGRVSPSS
  SSDIASSLGESIESGPLSDILSDEESSMPLAGMKKYADEKSERASSSEKNESHSATKSALIQKLMQDIQH
  QDNYEAIWEKIEGFVNKLDEFIQWLNEAMETTENWTPPKAEMDDLKLYLETHLSFKLNVDSHCALKEAVE
  EEGHQLLELIASHKAGLKDMLRMIASQWKELQRQIKRQHSWILRALDTIKAEILATDVSVEDEEGTGSPK
  AEVQLCYLEAQRDAVEQMSLKLYSEQYTSSSKRKEEFADMSKVHSVGSNGLLDFDSEYQELWDWLIDMES
  LVMDSHDLMMSEEQQQHLYKRYSVEMSIRHLKKTELLSKVEALKKGGVLLPNDLLEKVDSINEKWELLGK
  TLGEKIQDTMAGHSGSSPRDLLSPESGSLVRQLEVRIKELKGWLRDTELFIFNSCLRQEKEGTMNTEKQL
  QYFKSLCREIKQRRRGVASILRLCQHLLDDRETCNLNADHQPMQLIIVNLERRWEAIVMQAVQWQTRLQK
  KMGKESETLNVIDPGLMDLNGMSEDALEWDEMDISNKLISLNEESNDLDQELQPVIPSLKLGETSNEDPG
  YDEEADNHGGSQYASNITAPSSPHIYQVYSLHNVELYEDNHMPFLKNNPKVTGMTQPNVLTKSLSKDSSF
  SSTKSLPDLLGGSNLVKPCACHGGDMSQNSGSESGIVSEGDTETTTNSEMCLLNAVDGSPSNLETEHLDP
  QMGDAVNVLKQKFTDEGESIKLPNSSQSSISPVGCVNGKVGDLNSITKHTPDCLGEELQGKHDVFTFYDY
  SYLQGSKLKLPMIMKQSQSEKAHVEDPLLRGFYFDKKSCKSKHQTTELQPDVPPHERILASASHEMDRIS
  YKSGNIEKTFTGMQNAKQLSLLSHSSSIESLSPGGDLFGLGIFKNGSDSLQRSTSLESWLTSYKSNEDLF
  SCHSSGDISVSSGSVGELSKRTLDLLNRLENIQSPSEQKIKRSVSDITLQSSSQKMSFTGQMSLDIASSI
  NEDSAASLTELSSSDELSLCSEDIVLHKNKIPESNASFRKRLTRSVADESDVNVSMIVNVSCTSACTDDE
  DDSDLLSSSTLTLTEEELCIKDEDDDSSIATDDEIYEDCTLMSGLDYIKNELQTWIRPKLSLTRDKKRCN
  VSDEMKGSKDISSSEMTNPSDTLNIETLLNGSVKRVSENNGNGKNSSHTHELGTKRENKKTIFKVNKDPY
  VADMENGNIEGIPERQKGKPNVTSKVSENLGSHGKEISESEHCKCKALMDSLDDSNTAGKEFVSQDVRHL
  PKKCPNHHHFENQSTASTPTEKSFSELALETRFNNRQDSDALKSSDDAPSMAGKSAGCCLALEQNGTEEN
  ASISDISCCNCEPDVFHQKDAEDCSVHNFVKEIIDMASTALKSKSQPENEVAAPTSLTQIKEKVLEHSHR
  PIQLRKGDFYSYLSLSSHDSDCGEVTNYIEEKSSTPLPLDTTDSGLDDKEDIECFFEACVEGDSDGEEPC
  YSSAPPNESAVPSEAAMPLQATACSSEFSDSSLSADDADTVALSSPSSQERAEVGKEVNGLPQTSSGCAE
  NLEFTPSKLDSEKESSGKPGESGMPEEHNAASAKSKVQDLSLKANQPTDKAALHPSPKTLTCEENLLNLH
  EKRHRNMHR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004274
• ORF Size: 6957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004274.2, NM_004274.3, NM_004274.4, NP_004265.3
• RefSeq Size: 10342
• RefSeq ORF: 6960
• Locus ID: 9472
• Domains: spectrin
• Protein Families: Druggable Genome
• MW: 256.5 kDa
• Gene Summary: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is highly expressed in various brain regions and cardiac and skeletal muscle. It is specifically localized to the sarcoplasmic reticulum and nuclear membrane, and is involved in anchoring PKA to the nuclear membrane or sarcoplasmic reticulum. [provided by RefSeq, Jul 2008]