TDIF2 (DNTTIP2) (NM_014597) Human Tagged ORF Clone

CAT#: RC216436

DNTTIP2 (Myc-DDK-tagged)-Human deoxynucleotidyltransferase, terminal, interacting protein 2 (DNTTIP2)

Product Images

Western blot validation of overexpression lysate (Cat# LY415177) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216436 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNTTIP2
• Synonyms: ERBP; FCF2; HSU15552; LPTS-RP2; TdIF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216436 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGTTACCAGATCTGCACGGGCTAAGGCCAGCATCCAAGCCGCGTCGGCTGAAAGTTCCGGGCAAA
  AGAGTTTTGCTGCTAATGGGATTCAAGCGCATCCAGAAAGTAGTACTGGATCTGATGCCCGAACTACTGC
  TGAATCACAGACCACTGGGAAGCAAAGTTTAATCCCTAGAACTCCTAAAGCTAGAAAGAGGAAGAGCAGA
  ACTACAGGCTCACTACCAAAGGGGACTGAACCATCTACGGATGGAGAGACCTCTGAGGCAGAGTCAAATT
  ATTCTGTGTCTGAGCACCATGATACCATTTTAAGGGTAACTAGGAGAAGGCAGATCTTAATTGCATGCTC
  CCCAGTGTCCAGTGTTAGGAAAAAGCCGAAAGTAACTCCAACAAAGGAGTCTTACACTGAAGAAATAGTG
  TCTGAAGCAGAATCTCATGTTTCAGGTATTTCTAGAATTGTGCTTCCCACAGAAAAAACTACAGGAGCCA
  GAAGAAGTAAGGCTAAATCTCTGACAGATCCAAGCCAAGAATCTCATACAGAAGCTATATCTGATGCTGA
  GACATCAAGCTCAGACATTTCATTCTCTGGAATTGCAACTAGAAGAACCAGGAGTATGCAGAGGAAATTA
  AAGGCACAAACTGAAAAGAAAGATAGTAAGATTGTACCAGGAAATGAGAAACAGATCGTGGGTACACCTG
  TGAATTCAGAGGATTCAGATACCAGACAAACTTCCCATTTACAAGCAAGATCTCTTTCTGAGATAAATAA
  GCCAAATTTCTATAATAATGACTTTGATGATGATTTCTCCCACAGAAGTTCAGAAAATATATTAACAGTG
  CACGAACAGGCCAATGTTGAATCTCTTAAAGAAACAAAACAGAATTGTAAGGATTTGGATGAAGATGCCA
  ATGGAATAACAGATGATGGGAAAGAAATTAATGAGAAAAGTTCTCAGCTGAAGAATCTTTCTGAACTTCA
  GGACACTAGCCTTCAACAGTTAGTTTCTCAGAGACATTCAACCCCCCAAAATAAAAATGCTGTATCAGTG
  CACTCTAATCTGAACTCTGAGGCTGTAATGAAATCATTAACTCAAACATTTGCAACTGTGGAAGTAGGCA
  GATGGAATAACAACAAAAAGAGCCCCATAAAAGCAAGTGACTTGACAAAGTTTGGTGATTGTGGTGGTAG
  TGATGATGAAGAAGAGTCCACAGTTATAAGTGTCAGTGAAGACATGAACAGTGAAGGGAATGTAGATTTT
  GAATGTGATACCAAACTATACACGTCTGCGCCCAACACATCTCAGGGTAAAGATAATTCTGTCTTACTAG
  TTCTCAGCAGTGATGAAAGCCAACAGTCTGAAAACAGTGAGAATGAAGAGGATACTTTATGTTTTGTTGA
  AAATAGTGGCCAAAGGGAGTCATTAAGTGGAGACACAGGAAGTCTGTCATGTGACAATGCATTGTTTGTA
  ATTGACACAACTCCTGGAATGAGTGCTGATAAAAATTTTTACTTGGAAGAGGAAGACAAGGCAAGTGAGG
  TTGCCATTGAGGAAGAAAAAGAAGAGGAAGAGGATGAAAAAAGTGAAGAAGATTCATCAGACCATGACGA
  AAATGAAGATGAGTTTAGTGATGAAGAAGACTTCCTAAATAGCACAAAGGCTAAACTTCTGAAGTTGACA
  AGCAGCAGCATAGACCCTGGTCTGAGTATCAAGCAGTTGGGTGGTTTGTATATTAATTTCAATGCAGATA
  AACTACAGTCTAACAAGAGAACCCTAACACAGATCAAGGAGAAAAAGAAAAATGAGCTTCTGCAGAAAGC
  CGTCATTACACCTGATTTTGAAAAAAACCACTGTGTTCCACCATATAGTGAATCAAAGTATCAACTTCAG
  AAAAAACGCAGAAAAGAACGACAAAAAACAGCAGGGGATGGCTGGTTTGGTATGAAAGCTCCAGAAATGA
  CAAATGAACTGAAAAATGATCTCAAAGCACTGAAGATGAGAGCCAGCATGGACCCGAAAAGATTTTACAA
  GAAAAATGATAGAGATGGCTTCCCCAAGTACTTCCAGATTGGAACCATTGTTGACAATCCAGCTGATTTC
  TACCATTCACGAATTCCCAAGAAGCAAAGGAAAAGAACTATTGTGGAAGAACTGCTGGCTGATTCTGAAT
  TCAGAAGATACAACCGAAGGAAGTACTCAGAGATCATGGCTGAAAAAGCAGCAAATGCAGCAGGAAAAAA
  GTTCCGAAAGAAGAAGAAATTTCGCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216436 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVVTRSARAKASIQAASAESSGQKSFAANGIQAHPESSTGSDARTTAESQTTGKQSLIPRTPKARKRKSR
  TTGSLPKGTEPSTDGETSEAESNYSVSEHHDTILRVTRRRQILIACSPVSSVRKKPKVTPTKESYTEEIV
  SEAESHVSGISRIVLPTEKTTGARRSKAKSLTDPSQESHTEAISDAETSSSDISFSGIATRRTRSMQRKL
  KAQTEKKDSKIVPGNEKQIVGTPVNSEDSDTRQTSHLQARSLSEINKPNFYNNDFDDDFSHRSSENILTV
  HEQANVESLKETKQNCKDLDEDANGITDDGKEINEKSSQLKNLSELQDTSLQQLVSQRHSTPQNKNAVSV
  HSNLNSEAVMKSLTQTFATVEVGRWNNNKKSPIKASDLTKFGDCGGSDDEEESTVISVSEDMNSEGNVDF
  ECDTKLYTSAPNTSQGKDNSVLLVLSSDESQQSENSENEEDTLCFVENSGQRESLSGDTGSLSCDNALFV
  IDTTPGMSADKNFYLEEEDKASEVAIEEEKEEEEDEKSEEDSSDHDENEDEFSDEEDFLNSTKAKLLKLT
  SSSIDPGLSIKQLGGLYINFNADKLQSNKRTLTQIKEKKKNELLQKAVITPDFEKNHCVPPYSESKYQLQ
  KKRRKERQKTAGDGWFGMKAPEMTNELKNDLKALKMRASMDPKRFYKKNDRDGFPKYFQIGTIVDNPADF
  YHSRIPKKQRKRTIVEELLADSEFRRYNRRKYSEIMAEKAANAAGKKFRKKKKFRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014597
• ORF Size: 2268 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014597.3, NM_014597.4, NP_055412.2
• RefSeq Size: 2725 bp
• RefSeq ORF: 2271 bp
• Locus ID: 30836
• Protein Families: Druggable Genome
• MW: 84.5 kDa
• Gene Summary: This gene is thought to be involved in chromatin remodeling and gene transcription. The encoded nuclear protein binds to and enhances the transcriptional activity of the estrogen receptor alpha, and also interacts with terminal deoxynucleotidyltransferase. The expression profile of this gene is a potential biomarker for chronic obstructive pulmonary disease. [provided by RefSeq, Dec 2010]