CCDC50 (NM_178335) Human Tagged ORF Clone

CAT#: RC216523

CCDC50 (Myc-DDK-tagged)-Human coiled-coil domain containing 50 (CCDC50), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY405969) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216523 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC50
• Synonyms: C3orf6; DFNA44; YMER
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216523 representing NM_178335
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAGTCAGCATCGACCAGTCCAAGCTGCCTGGAGTCAAGGAAGTATGCCGAGATTTTGCTGTCC
  TGGAGGACCACACCCTGGCTCACAGCCTGCAGGAACAAGAGATTGAGCATCATTTGGCATCGAACGTTCA
  GCGGAACCGTTTGGTCCAGCATGATCTCCAGGTGGCTAAGCAGCTCCAAGAGGAAGATCTGAAAGCGCAG
  GCCCAGCTCCAGAAGCGCTACAAAGACCTTGAACAACAAGACTGTGAAATTGCTCAGGAAATTCAGGAGA
  AGCTGGCTATTGAGGCAGAGAGACGACGCATTCAGGAGAAGAAGGATGAGGACATAGCTCGCCTTTTGCA
  AGAAAAGGAGTTACAGGAAGAGAAAAAGAGAAAGAAACACTTTCCAGAGTTCCCTGCAACCCGTGCTTAT
  GCAGATAGTTACTATTATGAAGATGGAGACCAACCAGGGTCAAGGAGGGCCAGGGAATTGGGTTCTGGAT
  TCTCAAGACCTTGTAGACTCCAAAGAGATGGAAAGACTGTGAAGCACAAGAAAGAGAAACCAGAACATCC
  ACTGGAGAACTTGGAAGAGCCAGAACAACATTGTTCATCGAAGAGATCCCTGTCATCCTCTAGCTCGGGC
  AAAGGGAGGGACAATCCCCATATTAACAATGAGCAGCATGAAAGGAAACGGTCCACTCAGGAGAGGCCTC
  GGAGACCTCTGCTTCCCACGATCAGTGGTGAAGTGTTTCTGAGCACTGAATGTGATGACTGGGAGACTAA
  GATTAACCATCAGACTCGAAATTGGGAAAAACAGTCTCGACACCAAGATCGACTTTCACCCAAGTCCTCA
  CAAAAAGCAGGGCTTCACTGCAAGGAAGTTGTATATGGGAGGGACCATGGGCAAGGTGAGCACAGAAAAA
  GGAGACACAGGCCCAGGACTCCTCCATTCTCAGAGAGTGAGGAGCAGCTCCACCTCCATGACGCAGGAAT
  GAAGCCAAGAGTGATGAAAGAAGCTGTATCTACTCCATCACGAATGGCCCACAGGGATCAGGAATGGTAT
  GATGCTGAAATTGCCAGAAAACTGCAAGAAGAAGAACTTTTGGCTACCCAGGTGGACATGAGAGCCGCTC
  AAGTAGCTCAAGATGAAGAAATCGCTCGACTTCTAATGGCTGAAGAAAAGAAAGCTTACAAAAAAGCCAA
  GGAGCGGGAGAAATCATCTTTGGACAAAAGAAAGCAAGACCCCGAGTGGAAGCCAAAAACAGCTAAAGCA
  GCAAATTCCAAGTCAAAAGAGAGTGATGAACCTCACCATTCTAAGAATGAAAGGCCAGCACGGCCACCAC
  CACCTATCATGACAGATGGTGAAGATGCGGATTACACTCATTTTACAAACCAGCAGAGTTCCACACGGCA
  TTTCTCAAAATCAGAGTCCTCTCATAAAGGTTTTCATTACAAACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216523 representing NM_178335
  Red=Cloning site Green=Tags(s)
  
  MAEVSIDQSKLPGVKEVCRDFAVLEDHTLAHSLQEQEIEHHLASNVQRNRLVQHDLQVAKQLQEEDLKAQ
  AQLQKRYKDLEQQDCEIAQEIQEKLAIEAERRRIQEKKDEDIARLLQEKELQEEKKRKKHFPEFPATRAY
  ADSYYYEDGDQPGSRRARELGSGFSRPCRLQRDGKTVKHKKEKPEHPLENLEEPEQHCSSKRSLSSSSSG
  KGRDNPHINNEQHERKRSTQERPRRPLLPTISGEVFLSTECDDWETKINHQTRNWEKQSRHQDRLSPKSS
  QKAGLHCKEVVYGRDHGQGEHRKRRHRPRTPPFSESEEQLHLHDAGMKPRVMKEAVSTPSRMAHRDQEWY
  DAEIARKLQEEELLATQVDMRAAQVAQDEEIARLLMAEEKKAYKKAKEREKSSLDKRKQDPEWKPKTAKA
  ANSKSKESDEPHHSKNERPARPPPPIMTDGEDADYTHFTNQQSSTRHFSKSESSHKGFHYKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178335
• ORF Size: 1446 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_178335.1, NM_178335.2, NP_848018.1
• RefSeq Size: 2454
• RefSeq ORF: 1449
• Locus ID: 152137
• MW: 56.2 kDa
• Gene Summary: This gene encodes a soluble, cytoplasmic, tyrosine-phosphorylated protein with multiple ubiquitin-interacting domains. Mutations in this gene cause nonsyndromic, postlingual, progressive sensorineural DFNA44 hearing loss. In mouse, the protein is expressed in the inner ear during development and postnatal maturation and associates with microtubule-based structures. This protein may also function as a negative regulator of NF-kB signaling and as an effector of epidermal growth factor (EGF)-mediated cell signaling. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]