CDK5RAP1 (NM_016408) Human Tagged ORF Clone

CAT#: RC216600

CDK5RAP1 (Myc-DDK-tagged)-Human CDK5 regulatory subunit associated protein 1 (CDK5RAP1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY414004) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216600 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDK5RAP1
• Synonyms: C20orf34; C42; CGI-05; HSPC167
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216600 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCTTTACAGTGTGTCCTCCAAGTGCAGAGGTCTCTGGGGTGGGGACCATTGGCCTCTGTGTCTT
  GGCTGTCGCTGAGGATGTGCAGGGCACACAGCAGTCTCTCTAGTACCATGTGTCCCAGTCCAGAGAGGCA
  GGAGGATGGAGCTCGGAAGGATTTCAGCTCCAGGCTGGCTGCTGGACCGACTTTTCAACATTTTTTAAAA
  AGTGCCTCAGCTCCTCAGGAGAAGCTGTCTTCAGAAGTGGAAGACCCACCTCCCTATCTCATGATGGATG
  AACTTCTTGGAAGGCAGAGAAAAGTCTACCTCGAGACCTATGGCTGCCAGATGAATGTGAATGACACAGA
  GATAGCCTGGTCCATCTTACAGAAGAGTGGCTACCTGCGGACCAGTAACCTCCAAGAGGCAGATGTGATT
  CTCCTTGTCACGTGCTCTATCAGGGAGAAGGCTGAGCAGACCATCTGGAACCGTTTACATCAGCTTAAAG
  CCTTGAAGACAAGGCGGCCCCGCTCCCGGGTTCCTCTGAGGATTGGAATTCTAGGCTGCATGGCTGAGAG
  GTTGAAGGAGGAGATTCTCAACAGAGAGAAAATGGTAGATATTTTGGCTGGTCCTGATGCCTACCGGGAC
  CTTCCCCGGCTGCTGGCTGTTGCTGAGTCGGGCCAGCAAGCTGCCAACGTGCTGCTCTCTCTGGACGAGA
  CCTATGCTGATGTCATGCCAGTCCAGACAAGCGCCAGTGCCACGTCTGCCTTTGTGTCAATCATGCGAGG
  CTGTGACAACATGTGTAGCTACTGCATTGTTCCTTTCACCCGGGGCAGGGAGAGGAGTCGGCCTATTGCC
  TCCATTCTAGAGGAAGTGAAGAAGCTTTCTGAGCAGGGGCTGAAAGAAGTGACACTTCTTGGTCAGAATG
  TTAATAGTTTTCGGGACAATTCGGAGGTCCAGTTCAACAGTGCAGTGCCTACCAATCTCAGTCGTGGCTT
  TACCACCAACTATAAAACCAAGCAAGGAGGACTTCGTTTTGCTCATCTTCTGGATCAGGTCTCCAGAGTA
  GATCCTGAAATGAGGATCCGTTTTACCTCTCCCCACCCCAAGGATTTTCCTGATGAGGTTCTGCAGCTGA
  TTCATGAGAGAGATAACATCTGTAAACAGATCCACCTGCCAGCCCAGAGTGGAAGCAGCCGTGTGTTGGA
  GGCCATGCGGAGGGGATATTCAAGAGAAGCTTATGTGGAGTTAGTTCACCATATTAGAGAATCTATTCCA
  GGTGTGAGCCTCAGCAGCGATTTCATTGCTGGCTTTTGTGGTGAGACGGAGGAAGATCACGTCCAGACAG
  TCTCTTTGCTCCGGGAAGTTCAGTACAACATGGGCTTCCTCTTTGCCTACAGCATGAGACAGAAGACACG
  GGCATATCATAGGCTGAAGGATGATGTCCCGGAAGAGGTAAAATTAAGGCGTTTGGAGGAACTCATCACT
  ATCTTCCGAGAAGAAGCAACAAAAGCCAATCAGACCTCTGTGGGCTGTACCCAGTTGGTGCTAGTGGAAG
  GGCTCAGTAAACGCTCTGCCACTGACCTGTGTGGCAGGAATGATGGAAACCTTAAGGTGATCTTCCCTGA
  TGCAGAGATGGAGGATGTCAATAACCCTGGGCTCAGGGTCAGAGCCCAGCCTGGGGACTATGTGCTGGTG
  AAGATCACCTCAGCCAGTTCTCAGACACTTAGGGGACATGTTCTCTGCAGGACCACTCTGAGGGACTCTT
  CTGCATATTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216600 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHPLQCVLQVQRSLGWGPLASVSWLSLRMCRAHSSLSSTMCPSPERQEDGARKDFSSRLAAGPTFQHFLK
  SASAPQEKLSSEVEDPPPYLMMDELLGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVI
  LLVTCSIREKAEQTIWNRLHQLKALKTRRPRSRVPLRIGILGCMAERLKEEILNREKMVDILAGPDAYRD
  LPRLLAVAESGQQAANVLLSLDETYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIA
  SILEEVKKLSEQGLKEVTLLGQNVNSFRDNSEVQFNSAVPTNLSRGFTTNYKTKQGGLRFAHLLDQVSRV
  DPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVHHIRESIP
  GVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELIT
  IFREEATKANQTSVGCTQLVLVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQPGDYVLV
  KITSASSQTLRGHVLCRTTLRDSSAYC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016408
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016408.1, NM_016408.2, NM_016408.3, NP_057492.2
• RefSeq Size: 2143 bp
• RefSeq ORF: 1764 bp
• Locus ID: 51654
• Domains: TRAM, UPF0004, Elp3, Radical_SAM
• MW: 66.1 kDa
• Gene Summary: This gene encodes a regulator of cyclin-dependent kinase 5 activity. This protein has also been reported to modify RNA by adding a methylthio-group and may thus have a dual function as an RNA methylthiotransferase and as an inhibitor of cyclin-dependent kinase 5 activity. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013]