CNOT4 (NM_013316) Human Tagged ORF Clone

CAT#: RC217418

CNOT4 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY415669) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217418 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNOT4
• Synonyms: CLONE243; NOT4; NOT4H
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217418 representing NM_013316
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGCAGTCCTGATGCGAAGGAAGACCCTGTGGAGTGCCCTCTTTGCATGGAGCCCTTGGAGATAG
  ATGATATCAACTTTTTCCCTTGCACCTGTGGCTACCAGATTTGCCGATTTTGTTGGCATCGAATTCGCAC
  TGATGAAAATGGGCTTTGTCCTGCATGTAGAAAGCCATATCCAGAAGACCCAGCAGTTTATAAACCACTC
  TCCCAGGAAGAGCTGCAAAGGATAAAGAATGAGAAAAAACAGAAACAAAATGAGAGAAAACAGAAAATAT
  CAGAAAATCGCAAACATTTGGCTAGTGTACGTGTCGTACAAAAAAACCTCGTCTTTGTTGTAGGTTTATC
  TCAGCGCCTAGCAGACCCAGAGGTTTTAAAACGACCAGAATATTTTGGGAAGTTTGGTAAAATACATAAA
  GTTGTCATCAATAATAGCACATCATATGCAGGCTCACAGGGTCCAAGTGCCAGTGCTTATGTAACCTATA
  TCCGGTCAGAAGACGCTCTCAGAGCCATACAGTGTGTCAACAATGTGGTAGTAGATGGCAGAACACTTAA
  GGCATCTCTAGGTACAACAAAATACTGCAGTTACTTCTTAAAGAATATGCAGTGTCCAAAACCTGACTGC
  ATGTATCTTCATGAATTGGGGGATGAGGCGGCCAGCTTCACAAAAGAGGAAATGCAGGCGGGTAAACACC
  AAGAATATGAACAGAAGCTACTTCAAGAATTATATAAATTAAATCCCAATTTTCTTCAGCTATCTACGGG
  TTCAGTTGATAAAAATAAGAACAAAGTGACACCACTGCAGAGCCCCATTGACAAACCTTCAGATTCTCTC
  AGTATAGGGAACGGTGATAATTCCCAGCAGATATCTAACAGTGATACGCCTTCACCACCACCTGGTTTGT
  CAAAATCCAATCCAGTCATCCCCATCAGTTCATCCAATCACAGTGCACGGTCCCCTTTTGAAGGGGCAGT
  AACAGAGTCACAGTCGTTATTCTCAGACAATTTTCGCCATCCCAACCCTATCCCAAGTGGGCTTCCTCCT
  TTCCCCAGCTCCCCACAGACATCCAGTGACTGGCCTACAGCACCAGAACCACAGAGCCTCTTCACATCAG
  AAACAATCCCAGTATCATCCTCTACAGACTGGCAAGCAGCTTTTGGCTTTGGTTCTTCTAAACAACCAGA
  GGATGACTTGGGTTTTGATCCCTTCGATGTCACTCGAAAAGCCTTAGCAGACCTGATTGAGAAGGAACTG
  TCCGTTCAAGACCAACCTTCCCTTTCGCCCACATCTCTTCAGAACTCCTCTTCACACACTACAACCGCCA
  AAGGTCCAGGCTCTGGATTCCTGCATCCTGCTGCAGCTACAAATGCCAATTCTCTCAATAGTACCTTTTC
  AGTCTTGCCCCAGAGGTTCCCTCAATTTCAGCAGCACCGAGCGGTTTATAATTCATTCAGTTTTCCAGGC
  CAGGCAGCCCGCTATCCTTGGATGGCCTTTCCACGCAATAGCATCATGCACTTGAACCACACAGCAAACC
  CCACCTCAAATAGTAATTTCTTGGACTTGAATCTCCCGCCACAGCACAACACAGGTCTGGGAGGGATCCC
  TGTAGCAGGTATTCCAGCGTCTTCAGGAAACAGTTTAGACTCTCTTCAAGATGACAATCCTCCACACTGG
  CTAAAATCCCTTCAGGCCCTCACAGAGATGGACGGCCCCAGCGCTGCTCCATCACAGACCCACCACAGCG
  CCCCCTTCAGCACACAGATCCCGCTGCACAGAGCCAGTTGGAATCCCTACCCTCCTCCTTCAAACCCTTC
  CAGCTTCCACTCCCCACCCCCAGGCTTTCAGACGGCCTTCAGACCCCCCAGCAAAACCCCCACAGATTTA
  CTACAGAGTTCAACACTGGACCGCCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217418 representing NM_013316
  Red=Cloning site Green=Tags(s)
  
  MSRSPDAKEDPVECPLCMEPLEIDDINFFPCTCGYQICRFCWHRIRTDENGLCPACRKPYPEDPAVYKPL
  SQEELQRIKNEKKQKQNERKQKISENRKHLASVRVVQKNLVFVVGLSQRLADPEVLKRPEYFGKFGKIHK
  VVINNSTSYAGSQGPSASAYVTYIRSEDALRAIQCVNNVVVDGRTLKASLGTTKYCSYFLKNMQCPKPDC
  MYLHELGDEAASFTKEEMQAGKHQEYEQKLLQELYKLNPNFLQLSTGSVDKNKNKVTPLQSPIDKPSDSL
  SIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDNFRHPNPIPSGLPP
  FPSSPQTSSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKEL
  SVQDQPSLSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRFPQFQQHRAVYNSFSFPG
  QAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPVAGIPASSGNSLDSLQDDNPPHW
  LKSLQALTEMDGPSAAPSQTHHSAPFSTQIPLHRASWNPYPPPSNPSSFHSPPPGFQTAFRPPSKTPTDL
  LQSSTLDRH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013316
• ORF Size: 1917 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013316.1, NM_013316.2, NM_013316.3, NP_037448.2
• RefSeq Size: 3333 bp
• RefSeq ORF: 1920 bp
• Locus ID: 4850
• Cytogenetics: 7q33
• Domains: RRM, RRM_1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: RNA degradation
• MW: 70.2 kDa
• Gene Summary: 'The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]'