CARD14 (NM_024110) Human Tagged ORF Clone

CAT#: RC217455

CARD14 (Myc-DDK-tagged)-Human caspase recruitment domain family, member 14 (CARD14), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY411344) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217455 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CARD14 protein (Cat# TP317455). The protein was produced from HEK293T cells transfected with CARD14 cDNA clone (Cat# RC217455) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CARD14
• Synonyms: BIMP2; CARMA2; PRP; PSORS2; PSS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217455 representing NM_024110
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAACTGTGCCGCAGGGACTCCGCACTCACGGCACTGGACGAGGAGACACTGTGGGAGATGATGG
  AGAGCCACCGCCACAGGATCGTACGCTGCATCTGCCCCAGCCGCCTCACCCCCTACCTGCGCCAGGCCAA
  GGTGCTGTGCCAGCTGGACGAGGAGGAGGTGCTGCACAGCCCCCGGCTCACCAACAGCGCCATGCGGGCC
  GGGCACTTGCTGGATTTGCTGAAGACTCGAGGGAAGAACGGGGCCATCGCCTTCCTGGAGAGCCTGAAGT
  TCCACAACCCTGACGTCTACACCCTGGTCACCGGGCTGCAGCCTGATGTTGACTTCAGTAACTTTAGCGG
  TCTCATGGAGACATCCAAGCTGACCGAGTGCCTGGCTGGGGCCATCGGCAGCCTGCAGGAGGAGCTGAAC
  CAGGAAAAGGGGCAGAAGGAGGTGCTGCTGCGGCGGTGCCAGCAGCTGCAGGAGCACCTGGGCCTGGCCG
  AGACCCGTGCCGAGGGCCTGCACCAGCTGGAGGCTGACCACAGCCGCATGAAGCGTGAGGTTAGCGCACA
  CTTCCATGAGGTGCTGAGGCTGAAGGACGAGATGCTCAGCCTCTCGCTGCACTATAGCAATGCGCTGCAG
  GAAAAGGAGCTGGCCGCCTCACGCTGCCGCAGCCTGCAGGAGGAGCTGTATCTACTGAAGCAGGAGCTGC
  AGCGAGCCAACATGGTTTCCTCCTGTGAGCTGGAATTGCAAGAGCAGTCCCTGAGGACAGCCAGCGACCA
  GGAGTCCGGGGATGAGGAGCTGAACCGCCTGAAGGAGGAGAATGAGAAACTGCGCTCGCTGACTTTCAGC
  CTGGCGGAGAAGGACATTCTGGAGCAGAGCCTGGACGAGGCGCGGGGGAGCCGACAGGAGCTGGTGGAGC
  GCATCCACTCGCTGCGGGAGCGGGCCGTGGCTGCCGAGAGGCAGCGAGAGCAGTACTGGGAAGAGAAGGA
  ACAGACCCTGCTGCAGTTCCAGAAGAGTAAGATGGCCTGCCAACTCTACAGGGAGAAGGTGAATGCGCTG
  CAGGCCCAGGTGTGCGAGCTGCAGAAGGAGCGAGACCAGGCGTACTCCGCGAGGGACAGTGCTCAGAGGG
  AGATTTCCCAGAGCCTGGTGGAGAAGGACTCCCTCCGCAGGCAGGTGTTCGAGCTGACGGACCAGGTCTG
  CGAGCTGCGCACACAGCTTCGCCAGCTGCAGGCAGAGCCTCCGGGTGTGCTCAAGCAGGAAGCCAGGACC
  AGGGAGCCCTGTCCACGGGAGAAGCAGCGGCTGGTGCGGATGCATGCCATCTGCCCCAGAGACGACAGCG
  ACTGCAGCCTCGTCAGCTCCACAGAGTCTCAGCTCTTGTCGGACCTGAGTGCCACGTCCAGCCGCGAGCT
  GGTGGACAGCTTCCGCTCCAGCAGCCCCGCGCCCCCCAGCCAGCAGTCCCTGTACAAGCGGGTGGCCGAG
  GACTTCGGGGAAGAACCCTGGTCTTTCAGCAGCTGCCTGGAGATCCCGGAGGGAGACCCGGGAGCCCTGC
  CGGGAGCTAAGGCAGGCGACCCACACCTGGATTATGAGCTCCTAGACACGGCAGACCTTCCGCAGCTGGA
  AAGCAGCCTGCAGCCAGTCTCCCCTGGAAGGCTTGATGTCTCGGAGAGCGGCGTCCTCATGCGGCGGAGG
  CCAGCCCGCAGGATCCTGAGCCAGGTCACCATGCTGGCGTTCCAGGGGGATGCATTGCTGGAGCAGATCA
  GCGTCATCGGCGGGAACCTCACGGGCATCTTCATCCACCGGGTCACCCCGGGCTCGGCGGCGGACCAGAT
  GGCCTTGCGCCCGGGCACCCAGATTGTGATGGTTGATTACGAAGCCTCAGAGCCCTTGTTCAAGGCAGTC
  CTGGAGGACACGACCCTGGAGGAGGCCGTGGGGCTTCTCAGGAGGGTGGACGGCTTCTGCTGCCTGTCTG
  TGAAGGTCAACACGGACGGTTATAAGAGGCTACTCCAGGACCTGGAGGCCAAAGTGGCGACCTCGGGGGA
  CTCATTCTACATCCGGGTCAACCTGGCCATGGAGGGCAGGGCCAAAGGGGAGCTGCAGGTGCATTGCAAC
  GAGGTCCTGCACGTCACCGACACCATGTTCCAGGGCTGCGGCTGCTGGCATGCCCACCGCGTGAACTCTT
  ACACCATGAAGGATACTGCCGCGCACGGCACCATCCCCAACTACTCCAGGGCTCAGCAGCAGCTCATAGC
  CCTCATCCAGGACATGACTCAGCAGTGCACCGTGACCCGCAAGCCATCTTCTGGGGGACCACAGAAGCTG
  GTCCGCATCGTCAGTATGGACAAAGCCAAGGCCAGCCCTCTGCGTTTGTCCTTTGACAGGGGCCAGTTGG
  ACCCCAGCAGGATGGAGGGCTCCAGCACGTGCTTCTGGGCCGAGAGCTGCCTCACCCTGGTGCCCTATAC
  CCTGGTGTGGCCCCATCGACCCGCCCGGCCCCGGCCTGTGCTCCTCGTGCCCAGGGCGGTTGGGAAGATC
  CTGAGCGAGAAACTGTGCCTCCTCCAAGGGTTTAAGAAGTGCCTGGCAGAGTACTTGAGCCAGGAGGAAT
  ATGAGGCCTGGAGCCAGAGAGGGGACATCATCCAGGAGGGAGAGGTGTCCGGGGGCCGCTGCTGGGTGAC
  CCGCCATGCTGTGGAGTCCCTCATGGAAAAGAACACCCATGCCCTCCTGGACGTCCAGCTGGACAGTGTC
  TGCACCCTGCACAGGATGGACATCTTCCCCATCGTCATCCACGTCTCTGTCAACGAGAAGATGGCAAAGA
  AGCTCAAGAAGGGCCTACAGCGGTTGGGCACCTCAGAGGAGCAGCTCCTGGAGGCTGCGAGGCAGGAGGA
  GGGAGACCTGGACCGGGCGCCCTGTCTATACAGCAGCCTGGCTCCTGACGGCTGGAGCGACCTGGACGGC
  CTGCTCAGCTGTGTCCGCCAGGCCATCGCCGACGAGCAGAAGAAGGTGGTGTGGACGGAGCAGAGCCCCC
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217455 representing NM_024110
  Red=Cloning site Green=Tags(s)
  
  MGELCRRDSALTALDEETLWEMMESHRHRIVRCICPSRLTPYLRQAKVLCQLDEEEVLHSPRLTNSAMRA
  GHLLDLLKTRGKNGAIAFLESLKFHNPDVYTLVTGLQPDVDFSNFSGLMETSKLTECLAGAIGSLQEELN
  QEKGQKEVLLRRCQQLQEHLGLAETRAEGLHQLEADHSRMKREVSAHFHEVLRLKDEMLSLSLHYSNALQ
  EKELAASRCRSLQEELYLLKQELQRANMVSSCELELQEQSLRTASDQESGDEELNRLKEENEKLRSLTFS
  LAEKDILEQSLDEARGSRQELVERIHSLRERAVAAERQREQYWEEKEQTLLQFQKSKMACQLYREKVNAL
  QAQVCELQKERDQAYSARDSAQREISQSLVEKDSLRRQVFELTDQVCELRTQLRQLQAEPPGVLKQEART
  REPCPREKQRLVRMHAICPRDDSDCSLVSSTESQLLSDLSATSSRELVDSFRSSSPAPPSQQSLYKRVAE
  DFGEEPWSFSSCLEIPEGDPGALPGAKAGDPHLDYELLDTADLPQLESSLQPVSPGRLDVSESGVLMRRR
  PARRILSQVTMLAFQGDALLEQISVIGGNLTGIFIHRVTPGSAADQMALRPGTQIVMVDYEASEPLFKAV
  LEDTTLEEAVGLLRRVDGFCCLSVKVNTDGYKRLLQDLEAKVATSGDSFYIRVNLAMEGRAKGELQVHCN
  EVLHVTDTMFQGCGCWHAHRVNSYTMKDTAAHGTIPNYSRAQQQLIALIQDMTQQCTVTRKPSSGGPQKL
  VRIVSMDKAKASPLRLSFDRGQLDPSRMEGSSTCFWAESCLTLVPYTLVWPHRPARPRPVLLVPRAVGKI
  LSEKLCLLQGFKKCLAEYLSQEEYEAWSQRGDIIQEGEVSGGRCWVTRHAVESLMEKNTHALLDVQLDSV
  CTLHRMDIFPIVIHVSVNEKMAKKLKKGLQRLGTSEEQLLEAARQEEGDLDRAPCLYSSLAPDGWSDLDG
  LLSCVRQAIADEQKKVVWTEQSPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024110
• ORF Size: 3012 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024110.1, NM_024110.2, NM_024110.3, NM_024110.4, NP_077015.1
• RefSeq Size: 3917 bp
• RefSeq ORF: 3015 bp
• Locus ID: 79092
• Protein Families: Druggable Genome
• MW: 113.1 kDa
• Gene Summary: This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]