AFF4 (NM_014423) Human Tagged ORF Clone

CAT#: RC217692

AFF4 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 4 (AFF4)

Product Images

Western blot validation of overexpression lysate (Cat# LY402333) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217692 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AFF4
• Synonyms: AF5Q31; CHOPS; MCEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217692 representing NM_014423
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGTGAAGACCGGAATGTGCTGCGTATGAAAGAACGGGAAAGGCGGAATCAGGAAATTCAGCAGG
  GCGAAGACGCCTTCCCACCTAGCTCTCCTCTCTTTGCAGAGCCATACAAAGTTACTAGCAAAGAAGATAA
  GTTATCAAGTCGTATTCAGAGTATGCTTGGAAACTACGATGAAATGAAGGATTTCATAGGAGACAGATCT
  ATACCAAAGCTTGTTGCAATTCCCAAGCCTACAGTACCACCATCAGCAGATGAAAAATCTAACCCAAATT
  TCTTTGAACAGAGACATGGAGGCTCTCATCAGAGTAGCAAATGGACTCCAGTAGGACCCGCACCCAGCAC
  TTCTCAGTCTCAGAAACGGTCCTCAGGCTTACAGAGTGGACATAGTAGCCAGCGGACCAGCGCAGGTAGC
  AGTAGTGGCACTAACAGTAGTGGTCAGAGGCACGACCGTGAGTCATATAACAATAGTGGGAGCAGTAGCC
  GGAAAAAAGGCCAGCATGGATCAGAACACTCCAAATCACGTTCTTCCAGCCCTGGAAAACCCCAGGCTGT
  TTCTTCATTAAACTCTAGTCATTCCAGGTCTCATGGGAATGATCACCATAGCAAGGAACATCAACGCTCC
  AAATCACCTCGGGACCCTGATGCAAACTGGGATTCTCCTTCCCGTGTACCTTTTTCAAGTGGGCAGCACT
  CAACTCAATCTTTCCCACCCTCATTGATGTCAAAGTCCAATTCAATGTTACAGAAACCCACTGCCTATGT
  GCGGCCCATGGACGGACAGGAGTCCATGGAACCAAAGCTGTCCTCTGAGCACTACAGCAGCCAATCCCAT
  GGCAACAGCATGACTGAGCTGAAGCCCAGCAGCAAAGCACATCTCACCAAGCTGAAAATACCTTCCCAAC
  CACTGGATGCATCAGCTTCTGGTGATGTGAGCTGTGTGGATGAAATCCTAAAAGAGATGACGCATTCATG
  GCCTCCCCCTCTAACGGCTATTCATACACCATGCAAAACAGAACCTTCCAAATTTCCTTTTCCAACTAAG
  GAGTCTCAGCAGTCCAATTTTGGCACTGGAGAACAAAAAAGATATAATCCTTCTAAAACTTCAAATGGGC
  ACCAGTCTAAATCTATGTTAAAAGATGACTTAAAACTAAGCAGCAGTGAAGACAGTGATGGGGAACAGGA
  TTGTGATAAGACAATGCCGAGGAGTACACCAGGAAGTAACTCTGAACCTTCACACCATAATAGTGAAGGA
  GCAGATAACTCCAGGGATGATTCTAGTAGCCACAGTGGATCTGAAAGCAGCTCTGGATCTGACTCAGAGA
  GTGAAAGTAGTTCCAGTGACAGTGAGGCAAATGAGCCATCCCAGAGTGCATCTCCCGAGCCTGAACCCCC
  GCCAACAAACAAATGGCAACTTGATAATTGGCTGAATAAAGTGAACCCACATAAAGTGTCACCCGCCTCT
  TCAGTGGACAGTAACATCCCATCATCTCAAGGCTACAAAAAGGAAGGCCGAGAGCAGGGCACTGGGAATA
  GCTACACTGATACAAGTGGACCTAAAGAAACGAGTTCCGCTACTCCGGGACGAGACTCCAAAACCATCCA
  AAAGGGATCAGAAAGTGGGCGTGGGAGGCAGAAATCTCCTGCACAGAGTGACAGCACAACACAGAGAAGA
  ACTGTAGGCAAAAAACAACCCAAAAAGGCTGAGAAGGCAGCTGCTGAAGAGCCTCGTGGAGGCCTGAAGA
  TAGAAAGTGAAACCCCTGTAGACTTGGCTAGCAGCATGCCCTCCAGCAGACACAAAGCAGCCACCAAAGG
  CTCAAGGAAACCCAATATAAAGAAGGAGTCTAAGTCTTCCCCTCGACCTACAGCAGAGAAAAAGAAATAT
  AAGTCAACAAGTAAATCTTCCCAGAAATCAAGGGAAATCATAGAAACAGATACCTCATCCTCAGATTCAG
  ATGAAAGTGAGAGCCTTCCTCCTTCCTCACAAACTCCTAAGTACCCCGAGAGCAATAGGACTCCTGTTAA
  ACCCTCCTCAGTGGAGGAAGAAGATAGCTTTTTTCGGCAACGAATGTTCTCTCCTATGGAAGAGAAGGAA
  CTTCTTTCACCCCTCAGTGAGCCTGATGACAGGTACCCACTTATTGTGAAGATTGACCTGAATCTTTTGA
  CTAGAATACCAGGAAAGCCTTACAAAGAAACAGAGCCGCCCAAGGGGGAAAAGAAAAATGTGCCAGAAAA
  GCACACGAGAGAGGCTCAGAAACAAGCCTCAGAAAAAGTTTCCAACAAAGGCAAGAGGAAGCATAAGAAT
  GAAGATGATAACCGAGCCAGTGAGAGCAAGAAACCCAAAACGGAGGACAAGAATTCAGCAGGCCATAAGC
  CATCCAGCAACAGAGAGTCATCTAAGCAGAGTGCTGCAAAAGAAAAGGATTTGTTGCCTTCTCCCGCTGG
  GCCTGTTCCTTCAAAAGATCCAAAAACAGAGCATGGCTCTCGGAAGAGGACTATTAGTCAGTCTTCTTCC
  TTAAAGTCAAGCAGTAACAGCAACAAGGAGACGAGTGGCAGCAGCAAAAACAGTTCCTCCACATCAAAGC
  AGAAGAAGACCGAAGGGAAGACTTCCAGTAGCTCCAAGGAGGTTAAGGAAAAGGCTCCAAGTAGCTCCTC
  TAACTGTCCTCCATCTGCACCAACTCTTGATTCTTCTAAGCCTCGGAGAACAAAGCTTGTCTTTGATGAC
  AGAAATTATTCAGCAGACCATTATTTACAAGAAGCAAAAAAGCTAAAGCACAATGCAGATGCATTGTCTG
  ATAGGTTTGAGAAAGCTGTATACTATCTTGATGCTGTGGTATCTTTCATTGAATGTGGGAATGCATTAGA
  GAAGAATGCTCAGGAATCCAAATCCCCATTCCCTATGTATTCAGAGACGGTGGATCTCATCAAATACACT
  ATGAAGCTAAAGAATTACTTGGCACCAGATGCTACAGCTGCAGATAAACGACTCACAGTACTTTGCCTGC
  GATGCGAGTCTTTGCTGTACCTGAGGCTGTTCAAACTGAAGAAGGAAAATGCTCTGAAGTACTCAAAGAC
  ACTGACAGAGCACCTGAAGAATTCTTATAATAATTCTCAAGCACCATCGCCTGGCTTGGGAAGCAAAGCT
  GTGGGGATGCCTTCCCCTGTTTCTCCAAAGCTGTCACCAGGCAATTCAGGAAATTATTCATCTGGGGCCA
  GTAGTGCTTCTGCAAGTGGTTCTTCAGTGACCATTCCACAGAAGATCCACCAGATGGCAGCCAGCTATGT
  TCAGGTCACATCCAACTTCCTCTATGCCACCGAAATTTGGGACCAAGCTGAACAGCTTTCCAAAGAGCAA
  AAAGAATTCTTTGCTGAACTGGATAAAGTAATGGGCCCTCTCATCTTTAATGCAAGCATCATGACAGATC
  TAGTTCGTTATACCCGGCAGGGACTGCACTGGCTTCGCCAGGATGCCAAGTTGATATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217692 representing NM_014423
  Red=Cloning site Green=Tags(s)
  
  MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRS
  IPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGS
  SSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRS
  KSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSH
  GNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTK
  ESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEG
  ADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPAS
  SVDSNIPSSQGYKKEGREQGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRR
  TVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKY
  KSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKE
  LLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKN
  EDDNRASESKKPKTEDKNSAGHKPSSNRESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSS
  LKSSSNSNKETSGSSKNSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDD
  RNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSETVDLIKYT
  MKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTEHLKNSYNNSQAPSPGLGSKA
  VGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQ
  KEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014423
• ORF Size: 3489 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014423.1, NM_014423.2, NM_014423.3, NP_055238.1
• RefSeq Size: 9528 bp
• RefSeq ORF: 3492 bp
• Locus ID: 27125
• Domains: AF-4
• Protein Families: Transcription Factors
• MW: 127.3 kDa
• Gene Summary: The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011]