RBP2 (NM_004164) Human Tagged ORF Clone

CAT#: RC217970

RBP2 (Myc-DDK-tagged)-Human retinol binding protein 2, cellular (RBP2)

Product Images

Western blot validation of overexpression lysate (Cat# LY418171) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217970 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RBP2 protein (Cat# TP317970). The protein was produced from HEK293T cells transfected with RBP2 cDNA clone (Cat# RC217970) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RBP2
• Synonyms: CRABP-II; CRBP2; CRBPII; RBPC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217970 representing NM_004164
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAGGGACCAGAATGGAACCTGGGAGATGGAGAGTAATGAAAACTTTGAGGGCTACATGAAGGCCC
  TGGATATTGATTTTGCCACCCGCAAGATTGCAGTACGTCTCACTCAGACGAAGGTTATTGATCAAGATGG
  TGATAACTTCAAGACAAAAACCACTAGCACATTCCGCAACTATGATGTGGATTTCACTGTTGGAGTAGAG
  TTTGACGAGTACACAAAGAGCCTGGATAACCGGCATGTTAAGGCACTGGTCACCTGGGAAGGTGATGTCC
  TTGTGTGTGTGCAAAAGGGGGAGAAGGAGAACCGCGGCTGGAAGCAGTGGATTGAGGGGGACAAGCTGTA
  CCTGGAGCTGACCTGTGGTGACCAGGTGTGCCGTCAAGTGTTCAAAAAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217970 representing NM_004164
  Red=Cloning site Green=Tags(s)
  
  MTRDQNGTWEMESNENFEGYMKALDIDFATRKIAVRLTQTKVIDQDGDNFKTKTTSTFRNYDVDFTVGVE
  FDEYTKSLDNRHVKALVTWEGDVLVCVQKGEKENRGWKQWIEGDKLYLELTCGDQVCRQVFKKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004164
• ORF Size: 402 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004164.1, NM_004164.2, NP_004155.2
• RefSeq Size: 700 bp
• RefSeq ORF: 405 bp
• Locus ID: 5948
• Cytogenetics: 3q23
• MW: 15.5 kDa
• Gene Summary: 'This gene encodes an abundant protein present in the small intestinal epithelium. It is thought to participate in the uptake and/or intracellular metabolism of vitamin A. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. This protein may also modulate the supply of retinoic acid to the nuclei of endometrial cells during the menstrual cycle. [provided by RefSeq, Aug 2015]'