DAAM2 (NM_015345) Human Tagged ORF Clone

CAT#: RC220800

DAAM2 (Myc-DDK-tagged)-Human dishevelled associated activator of morphogenesis 2 (DAAM2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DAAM2
• Synonyms: dJ90A20A.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220800 representing NM_015345
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCCGCAAGAGGAGCCACCATGGCCTGGGCTTCCTGTGCTGCTTCGGGGGCAGTGACATCCCCG
  AAATCAACCTCCGGGACAACCACCCTCTGCAGTTCATGGAGTTCTCCAGCCCCATCCCGAACGCAGAGGA
  GCTCAACATCCGCTTTGCAGAGCTGGTGGATGAATTGGATCTCACTGACAAAAACCGAGAGGCTATGTTT
  GCACTGCCCCCTGAGAAGAAATGGCAGATCTACTGCAGCAAGAAGAAGGAGCAGGAGGACCCCAACAAGC
  TGGCAACCAGCTGGCCTGACTATTACATCGACCGCATCAATTCCATGGCTGCGATGCAGAGTCTGTACGC
  GTTTGATGAGGAGGAGACGGAGATGAGGAACCAAGTCGTGGAAGACCTGAAGACAGCCCTCCGGACACAG
  CCTATGAGGTTTGTGACCCGCTTCATTGAGCTGGAGGGCTTGACCTGTCTGCTAAATTTCCTCCGGAGCA
  TGGACCACGCCACCTGTGAGAGCCGCATCCACACCTCACTCATTGGCTGCATCAAAGCATTGATGAACAA
  CTCCCAGGGGCGGGCACATGTGCTGGCACAGCCTGAGGCCATTAGTACCATAGCCCAGAGCCTACGCACA
  GAGAACAGCAAGACCAAGGTGGCTGTGCTGGAGATCCTGGGTGCTGTGTGCCTCGTGCCTGGTGGCCACA
  AGAAGGTGCTGCAGGCCATGCTGCACTACCAGGTGTATGCAGCAGAGCGAACCCGCTTCCAGACCCTGCT
  GAACGAGCTAGACCGAAGTCTGGGCCGGTACCGGGATGAAGTGAATCTGAAAACAGCCATCATGTCCTTC
  ATCAATGCTGTCCTCAATGCTGGAGCTGGAGAGGATAATCTGGAGTTCCGCCTACATCTACGGTATGAAT
  TCCTGATGCTGGGTATACAGCCTGTGATTGACAAGCTCCGGCAACATGAAAATGCCATCCTGGACAAACA
  TTTAGACTTCTTCGAGATGGTGCGGAATGAGGATGACCTGGAGCTAGCCAGGAGGTTTGACATGGTCCAC
  ATCGACACCAAGAGTGCTTCCCAGATGTTTGAGTTGATCCACAAGAAGCTGAAGTACACGGAGGCCTACC
  CCTGCCTGCTCTCTGTGCTGCACCACTGCCTGCAGATGCCCTACAAACGGAACGGTGGCTACTTCCAGCA
  GTGGCAGCTCCTGGACCGCATCCTCCAGCAGATTGTCCTCCAGGATGAGCGGGGTGTGGACCCTGACCTG
  GCTCCCTTGGAGAACTTCAATGTCAAGAACATCGTCAACATGCTCATCAACGAGAATGAAGTGAAACAGT
  GGCGAGACCAGGCAGAGAAGTTCCGGAAAGAACACATGGAGCTTGTGAGCCGTCTGGAGAGGAAGGAGCG
  GGAATGCGAGACAAAGACATTGGAGAAGGAAGAGATGATGCGGACGCTGAACAAAATGAAGGACAAGCTG
  GCCCGGGAGTCCCAGGAGCTGCGCCAGGCTCGGGGACAAGTGGCAGAGCTGGTAGCCCAGCTCAGTGAAC
  TCTCAACAGGCCCTGTATCTTCCCCACCACCCCCTGGGGGCCCACTCACCTTGTCTTCCTCAATGACAAC
  CAATGACCTGCCTCCACCCCCTCCTCCTCTGCCCTTTGCCTGTTGTCCCCCTCCCCCACCACCACCCCTT
  CCTCCCGGGGGACCCCCGACTCCCCCAGGTGCCCCACCTTGCCTCGGCATGGGCCTGCCCCTCCCTCAGG
  ACCCCTACCCCAGCAGTGACGTCCCACTCAGGAAAAAGCGTGTCCCCCAGCCTTCTCACCCACTGAAGTC
  CTTCAACTGGGTGAAGCTGAATGAGGAGCGTGTCCCTGGCACCGTATGGAATGAGATTGATGACATGCAG
  GTATTTCGGATCCTGGACCTAGAGGATTTTGAAAAGATGTTTTCAGCCTACCAGAGGCACCAGAAAGAGC
  TGGGCTCCACTGAAGACATCTACCTGGCTTCCCGCAAGGTCAAAGAGCTGTCGGTCATTGATGGCCGGAG
  GGCCCAAAACTGCATCATCCTTCTTTCCAAGTTGAAGCTTTCTAACGAGGAGATCCGGCAGGCCATCTTG
  AAGATGGATGAGCAGGAGGACCTTGCTAAGGACATGCTGGAGCAGCTCCTCAAGTTCATCCCAGAGAAGA
  GTGACATTGACCTCCTGGAGGAGCACAAGCATGAAATTGAGCGGATGGCCCGTGCTGACCGCTTCCTCTA
  TGAAATGAGCAGGATTGACCACTACCAGCAGCGACTGCAAGCCCTCTTCTTCAAGAAGAAATTCCAGGAG
  CGGCTGGCTGAGGCAAAGCCCAAAGTGGAAGCCATCCTGTTGGCCTCCCGGGAGCTGGTCCGCAGCAAGC
  GTCTTAGACAGATGCTAGAGGTCATCCTAGCCATAGGCAACTTCATGAACAAAGGGCAGCGTGGGGGCGC
  CTACGGGTTCCGGGTGGCCAGCCTCAACAAGATCGCTGACACCAAGTCCAGCATCGACAGAAACATCTCT
  CTGCTCCATTACCTGATCATGATCCTGGAGAAGCATTTTCCTGATATTCTAAACATGCCTTCAGAGCTGC
  AACATCTTCCAGAAGCTGCCAAAGTCAACCTAGCAGAACTGGAGAAGGAGGTGGGCAACCTCAGGAGGGG
  CCTGAGAGCGGTGGAGGTGCTGGAGTATCAGAGGCGCCAGGTACGGGAGCCCAGTGACAAGTTTGTCCCT
  GTCATGAGCGACTTCATCACGGTGTCCAGCTTCAGCTTCTCCGAGCTGGAGGACCAGCTAAATGAGGCCA
  GGGACAAGTTCGCCAAGGCCTTGATGCACTTCGGGGAGCATGACAGCAAGATGCAGCCAGACGAATTCTT
  TGGCATCTTTGATACCTTCTTGCAGGCCTTCTCAGAGGCCCGGCAGGATCTAGAGGCCATGAGGAGGAGG
  AAGGAGGAGGAGGAGCGGCGGGCGCGCATGGAAGCCATGCTGAAGGAGCAGAGGGAACGTGAGCGGTGGC
  AGCGGCAGCGGAAGGTCCTGGCTGCAGGCAGCTCGCTGGAGGAGGGAGGAGAGTTCGATGACCTGGTGTC
  GGCCCTGCGCTCTGGGGAGGTCTTCGACAAGGACTTATGCAAGCTCAAGCGCAGCCGCAAGCGATCAGGG
  AGCCAGGCCCTGGAAGTTACCCGGGAGCGGGCAATAAACCGGCTAAATTAT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220800 representing NM_015345
  Red=Cloning site Green=Tags(s)
  
  MAPRKRSHHGLGFLCCFGGSDIPEINLRDNHPLQFMEFSSPIPNAEELNIRFAELVDELDLTDKNREAMF
  ALPPEKKWQIYCSKKKEQEDPNKLATSWPDYYIDRINSMAAMQSLYAFDEEETEMRNQVVEDLKTALRTQ
  PMRFVTRFIELEGLTCLLNFLRSMDHATCESRIHTSLIGCIKALMNNSQGRAHVLAQPEAISTIAQSLRT
  ENSKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQVYAAERTRFQTLLNELDRSLGRYRDEVNLKTAIMSF
  INAVLNAGAGEDNLEFRLHLRYEFLMLGIQPVIDKLRQHENAILDKHLDFFEMVRNEDDLELARRFDMVH
  IDTKSASQMFELIHKKLKYTEAYPCLLSVLHHCLQMPYKRNGGYFQQWQLLDRILQQIVLQDERGVDPDL
  APLENFNVKNIVNMLINENEVKQWRDQAEKFRKEHMELVSRLERKERECETKTLEKEEMMRTLNKMKDKL
  ARESQELRQARGQVAELVAQLSELSTGPVSSPPPPGGPLTLSSSMTTNDLPPPPPPLPFACCPPPPPPPL
  PPGGPPTPPGAPPCLGMGLPLPQDPYPSSDVPLRKKRVPQPSHPLKSFNWVKLNEERVPGTVWNEIDDMQ
  VFRILDLEDFEKMFSAYQRHQKELGSTEDIYLASRKVKELSVIDGRRAQNCIILLSKLKLSNEEIRQAIL
  KMDEQEDLAKDMLEQLLKFIPEKSDIDLLEEHKHEIERMARADRFLYEMSRIDHYQQRLQALFFKKKFQE
  RLAEAKPKVEAILLASRELVRSKRLRQMLEVILAIGNFMNKGQRGGAYGFRVASLNKIADTKSSIDRNIS
  LLHYLIMILEKHFPDILNMPSELQHLPEAAKVNLAELEKEVGNLRRGLRAVEVLEYQRRQVREPSDKFVP
  VMSDFITVSSFSFSELEDQLNEARDKFAKALMHFGEHDSKMQPDEFFGIFDTFLQAFSEARQDLEAMRRR
  KEEEERRARMEAMLKEQRERERWQRQRKVLAAGSSLEEGGEFDDLVSALRSGEVFDKDLCKLKRSRKRSG
  SQALEVTRERAINRLNY
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015345
• ORF Size: 3201 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015345.1, NM_015345.2, NM_015345.3, NP_056160.2
• RefSeq Size: 6215
• RefSeq ORF: 3204
• Locus ID: 23500
• Protein Pathways: Wnt signaling pathway
• MW: 123.2 kDa