CTNND1 (NM_001085458) Human Tagged ORF Clone

CAT#: RC222771

CTNND1 (Myc-DDK-tagged)-Human catenin (cadherin-associated protein), delta 1 (CTNND1), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CTNND1 (Cat# RC222771, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNND1(Cat# TA800807). Positive lysates LY421307 (100ug) and LC421307 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY421307) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222771 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CTNND1 protein (Cat# TP322771). The protein was produced from HEK293T cells transfected with CTNND1 cDNA clone (Cat# RC222771) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CTNND1
• Synonyms: BCDS2; CAS; CTNND; p120; p120(CAS); p120(CTN); P120CAS; P120CTN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222771 representing NM_001085458.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACGACTCAGAGGTGGAGTCGACCGCCAGCATCTTGGCCTCTGTGAAGGAACAAGAGGCCCAGTTT
  GAGAAGCTGACCCGGGCGCTGGAGGAGGAACGGCGCCACGTCTCGGCGCAGCTGGAACGCGTCCGGGTC
  TCACCACAAGATGCCAACCCACTCATGGCCAACGGCACACTCACCCGCCGGCATCAGAACGGCCGGTTT
  GTGGGCGATGCTGACCTTGAAAGACAGAAATTTTCAGATTTGAAACTCAACGGACCCCAGGATCACAGT
  CACCTTCTATATAGCACCATCCCCAGGATGCAGGAGCCGGGGCAGATTGTGGAGACCTACACGGAGGAG
  GATCCTGAGGGAGCCATGTCTGTAGTCTCTGTGGAGACCTCAGATGATGGGACCACTCGGCGCACAGAG
  ACCACGGTCAAGAAAGTAGTGAAGACTGTGACAACACGGACAGTACAGCCAGTCGCTATGGGACCAGAC
  GGGTTGCCTGTGGATGCTTCATCAGTTTCTAACAACTATATCCAGACTTTGGGTCGTGATTTCCGCAAG
  AATGGCAATGGGGGACCTGGTCCCTATGTGGGGCAAGCTGGCACTGCTACCCTTCCTAGGAACTTCCAC
  TACCCTCCTGATGGTTATAGTCGCCACTATGAAGATGGTTATCCAGGTGGCAGTGATAACTATGGCAGT
  CTGTCCCGGGTGACCCGCATTGAGGAGCGGTATAGGCCCAGCATGGAAGGCTACCGGGCACCTAGTAGA
  CAGGATGTGTATGGGCCCCAACCCCAGGTTCGGGTAGGTGGGAGCAGCGTGGATCTGCATCGCTTTCAT
  CCAGAGCCTTATGGGCTAGAGGATGACCAGCGTAGTATGGGCTATGATGACCTGGATTATGGTATGATG
  TCTGATTATGGCACTGCCCGTCGGACTGGGACACCCTCTGACCCTCGTCGGCGCCTCAGGAGCTATGAA
  GACATGATTGGTGAGGAGGTGCCATCGGATCAATACTACTGGGCTCCTTTGGCCCAGCATGAGCGAGGA
  AGTTTAGCAAGCTTGGATAGCCTGCGCAAAGGAGGGCCTCCACCTCCTAATTGGAGACAGCCAGAGCTG
  CCAGAGGTGATCGCCATGCTTGGATTCCGCTTGGATGCTGTCAAGTCCAATGCAGCTGCATACCTGCAA
  CACTTATGCTACCGCAATGACAAGGTGAAGACTGACGTGCGGAAGCTCAAGGGCATCCCAGTACTGGTG
  GGATTGTTAGACCATCCCAAAAAGGAAGTGCACCTTGGAGCCTGTGGAGCTCTCAAGAATATCTCTTTT
  GGACGTGACCAGGATAACAAGATTGCCATAAAAAACTGTGATGGTGTGCCTGCCCTTGTGCGATTGCTT
  CGAAAGGCTCGTGATATGGACCTTACTGAAGTTATTACCGGAACCCTGTGGAATCTTTCATCCCATGAC
  TCAATCAAAATGGAGATTGTGGACCATGCACTGCATGCCTTGACAGATGAAGTGATCATTCCTCATTCT
  GGTTGGGAGCGGGAACCTAATGAAGACTGTAAGCCACGCCATATTGAGTGGGAATCGGTGCTCACCAAC
  ACAGCTGGCTGCCTTAGGAATGTAAGCTCAGAGAGGAGTGAAGCTCGCCGGAAACTTCGGGAATGTGAT
  GGTTTAGTTGATGCCCTCATTTTCATTGTTCAGGCTGAGATTGGGCAGAAGGATTCAGACAGCAAGCTT
  GTAGAGAACTGTGTTTGCCTTCTTCGGAACTTATCATATCAAGTTCACCGGGAGATCCCACAGGCAGAG
  CGTTACCAAGAGGCAGCTCCCAATGTTGCCAACAATACTGGGCCACATGCTGCCAGTTGCTTTGGGGCC
  AAGAAGGGCAAAGATGAGTGGTTCTCCAGAGGGAAAAAACCTATAGAGGATCCAGCAAACGATACAGTG
  GATTTCCCTAAAAGAACGAGTCCAGCTCGAGGCTATGAGCTCTTATTTCAGCCAGAGGTGGTTCGGATA
  TACATCTCACTTCTTAAGGAGAGCAAGACTCCTGCCATCCTAGAAGCCTCAGCTGGAGCTATCCAGAAC
  TTGTGTGCTGGGCGCTGGACGTATGGTCGATACATCCGCTCTGCTCTGCGTCAAGAGAAGGCTCTTTCT
  GCCATAGCTGACCTCCTGACTAATGAACATGAACGGGTGGTGAAAGCTGCATCTGGAGCACTGAGAAAC
  CTGGCTGTGGATGCTCGCAACAAAGAATTAATTGGTAAACATGCTATTCCTAACTTGGTAAAGAATCTG
  CCAGGAGGACAGCAGAACTCCTCTTGGAATTTCTCTGAGGACACTGTCATCTCTATTTTGAACACTATC
  AACGAGGTTATCGCTGAGAACTTGGAGGCTGCCAAAAAGCTTCGAGAGACACAGGGTATTGAGAAGCTG
  GTGTTGATCAACAAATCAGGGAACCGCTCAGAAAAAGAAGTTCGAGCAGCAGCACTTGTATTACAGACA
  ATCTGGGGATATAAGGAACTGCGGAAGCCACTGGAAAAAGAAGGATGGAAGAAATCAGACTTTCAGGTG
  AATCTAAACAATGCTTCCCGAAGCCAGAGCAGTCATTCATATGATGATAGTACTCTCCCTCTCATTGAC
  CGGAACCAAAAATCAGATAAGAAACCTGATCGGGAAGAAATTCAGATGAGCAATATGGGATCAAACACA
  AAATCACTAGATAACAACTATTCCACACCAAATGAGAGAGGAGACCACAATAGAACACTGGATCGATCG
  GGGGATCTAGGCGACATGGAGCCATTGAAGGGAACAACACCCTTGATGCAGGACGAGGGGCAGGAATCT
  CTGGAGGAAGAGTTGGATGTGTTGGTTTTGGATGATGAGGGGGGCCAAGTGTCTTACCCCTCCATGCAG
  AAGATT
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC222771
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDDSEVESTASILASVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANPLMANGTLTRRHQNGRF
  VGDADLERQKFSDLKLNGPQDHSHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTE
  TTVKKVVKTVTTRTVQPVAMGPDGLPVDASSVSNNYIQTLGRDFRKNGNGGPGPYVGQAGTATLPRNFH
  YPPDGYSRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQPQVRVGGSSVDLHRFH
  PEPYGLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQHERG
  SLASLDSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLV
  GLLDHPKKEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHD
  SIKMEIVDHALHALTDEVIIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECD
  GLVDALIFIVQAEIGQKDSDSKLVENCVCLLRNLSYQVHREIPQAERYQEAAPNVANNTGPHAASCFGA
  KKGKDEWFSRGKKPIEDPANDTVDFPKRTSPARGYELLFQPEVVRIYISLLKESKTPAILEASAGAIQN
  LCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNL
  PGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQT
  IWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNT
  KSLDNNYSTPNERGDHNRTLDRSGDLGDMEPLKGTTPLMQDEGQESLEEELDVLVLDDEGGQVSYPSMQ
  KI
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC222771 also available, TP322771M
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001085458
• ORF Size: 2904 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001085458.1, NP_001078927.1
• RefSeq Size: 6363 bp
• RefSeq ORF: 2907 bp
• Locus ID: 1500
• UniProt ID: O60716
• Cytogenetics: 11q12.1
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Leukocyte transendothelial migration
• MW: 108.2 kDa
• Gene Summary: This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene. [provided by RefSeq, Dec 2010]