GAK (NM_005255) Human Tagged Lenti ORF Clone
CAT#: RC223572L4
- LentiORF®
Lenti ORF clone of Human cyclin G associated kinase (GAK), mGFP tagged
"NM_005255" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | GAK |
Synonyms | DNAJ26; DNAJC26 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC223572).
|
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene |
ACCN | NM_005255 |
ORF Size | 3933 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005255.1 |
RefSeq Size | 4331 bp |
RefSeq ORF | 3936 bp |
Locus ID | 2580 |
UniProt ID | O14976 |
Cytogenetics | 4p16.3 |
Domains | pkinase, TyrKc, DnaJ, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
MW | 143 kDa |
Gene Summary | In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC116876 | GAK (untagged)-Human cyclin G associated kinase (GAK) |
USD 2,660.00 |
|
SC323361 | GAK (untagged)-Kinase deficient mutant (K69M) of Human cyclin G associated kinase (GAK) |
USD 2,660.00 |
|
RC223572 | GAK (Myc-DDK-tagged)-Human cyclin G associated kinase (GAK) |
USD 1,380.00 |
|
RG223572 | GAK (GFP-tagged) - Human cyclin G associated kinase (GAK) |
USD 1,520.00 |
|
RC223572L1 | Lenti ORF clone of Human cyclin G associated kinase (GAK), Myc-DDK-tagged |
USD 1,956.00 |
|
RC223572L2 | Lenti ORF clone of Human cyclin G associated kinase (GAK), mGFP tagged |
USD 1,580.00 |
|
RC223572L3 | Lenti ORF clone of Human cyclin G associated kinase (GAK), Myc-DDK-tagged |
USD 1,580.00 |
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