CMAS (NM_018686) Human Tagged ORF Clone

CAT#: RC223608

CMAS (Myc-DDK-tagged)-Human cytidine monophosphate N-acetylneuraminic acid synthetase (CMAS)

Product Images

Western blot validation of overexpression lysate (Cat# LY402711) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223608 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CMAS protein (Cat# TP323608). The protein was produced from HEK293T cells transfected with CMAS cDNA clone (Cat# RC223608) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CMAS
• Synonyms: CSS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223608 representing NM_018686
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCGGTGGAGAAGGGGGCCGCCACCTCCGTCTCCAACCCGCGGGGGCGACCGTCCCGGGGCCGGC
  CGCCGAAGCTGCAGCGCAACTCTCGCGGCGGCCAGGGCCGAGGTGTGGAGAAGCCCCCGCACCTGGCAGC
  CCTAATTCTGGCCCGGGGAGGCAGCAAAGGCATCCCCCTGAAGAACATTAAGCACCTGGCGGGGGTCCCG
  CTCATTGGCTGGGTCCTGCGTGCGGCCCTGGATTCAGGGGCCTTCCAGAGTGTATGGGTTTCGACAGACC
  ATGATGAAATTGAGAATGTGGCCAAACAATTTGGTGCACAAGTTCATCGAAGAAGTTCTGAAGTTTCAAA
  AGACAGCTCTACCTCACTAGATGCCATCATAGAATTTCTTAATTATCATAATGAGGTTGACATTGTAGGA
  AATATTCAAGCTACTTCTCCATGTTTACATCCTACTGATCTTCAAAAAGTTGCAGAAATGATTCGAGAAG
  AAGGATATGATTCTGTTTTCTCTGTTGTGAGACGCCATCAGTTTCGATGGAGTGAAATTCAGAAAGGAGT
  TCGTGAAGTGACCGAACCTCTGAATTTAAATCCAGCTAAACGGCCTCGTCGACAAGACTGGGATGGAGAA
  TTATATGAAAATGGCTCATTTTATTTTGCTAAAAGACATTTGATAGAGATGGGTTACTTGCAGGGTGGAA
  AAATGGCATACTACGAAATGCGAGCTGAACATAGTGTGGATATAGATGTGGATATTGATTGGCCTATTGC
  AGAGCAAAGAGTATTAAGATATGGCTATTTTGGCAAAGAGAAGCTTAAGGAAATAAAACTTTTGGTTTGC
  AATATTGATGGATGTCTCACCAATGGCCACATTTATGTATCAGGAGACCAAAAAGAAATAATATCTTATG
  ATGTAAAAGATGCTATTGGGATAAGTTTATTAAAGAAAAGTGGTATTGAGGTGAGGCTAATCTCAGAAAG
  GGCCTGTTCAAAGCAGACGCTGTCTTCTTTAAAACTGGATTGCAAAATGGAAGTCAGTGTATCAGACAAG
  CTAGCAGTTGTAGATGAATGGAGAAAAGAAATGGGCCTGTGCTGGAAAGAAGTGGCATATCTTGGAAATG
  AAGTGTCTGATGAAGAGTGCTTGAAGAGAGTGGGCCTAAGTGGCGCTCCTGCTGATGCCTGTTCTACTGC
  CCAGAAGGCTGTTGGATACATTTGCAAATGTAATGGTGGCCGTGGTGCCATCCGAGAATTTGCAGAGCAC
  ATTTGCCTACTAATGGAAAAGGTTAATAATTCATGCCAAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223608 representing NM_018686
  Red=Cloning site Green=Tags(s)
  
  MDSVEKGAATSVSNPRGRPSRGRPPKLQRNSRGGQGRGVEKPPHLAALILARGGSKGIPLKNIKHLAGVP
  LIGWVLRAALDSGAFQSVWVSTDHDEIENVAKQFGAQVHRRSSEVSKDSSTSLDAIIEFLNYHNEVDIVG
  NIQATSPCLHPTDLQKVAEMIREEGYDSVFSVVRRHQFRWSEIQKGVREVTEPLNLNPAKRPRRQDWDGE
  LYENGSFYFAKRHLIEMGYLQGGKMAYYEMRAEHSVDIDVDIDWPIAEQRVLRYGYFGKEKLKEIKLLVC
  NIDGCLTNGHIYVSGDQKEIISYDVKDAIGISLLKKSGIEVRLISERACSKQTLSSLKLDCKMEVSVSDK
  LAVVDEWRKEMGLCWKEVAYLGNEVSDEECLKRVGLSGAPADACSTAQKAVGYICKCNGGRGAIREFAEH
  ICLLMEKVNNSCQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018686
• ORF Size: 1302 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018686.1, NM_018686.2, NM_018686.3, NM_018686.4, NM_018686.5, NP_061156.1
• RefSeq Size: 1741 bp
• RefSeq ORF: 1305 bp
• Locus ID: 55907
• Domains: CTP_transf_3
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Metabolic pathways
• MW: 48.2 kDa
• Gene Summary: This gene encodes an enzyme that converts N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc). This process is important in the formation of sialylated glycoprotein and glycolipids. This modification plays a role in cell-cell communications and immune responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]