MMS19 (NM_022362) Human Tagged ORF Clone

CAT#: RC224035

MMS19 (Myc-DDK-tagged)-Human MMS19 nucleotide excision repair homolog (S. cerevisiae) (MMS19)

Product Images

Western blot validation of overexpression lysate (Cat# LY411688) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224035 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MMS19
• Synonyms: CIAO4; hMMS19; MET18; MMS19L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224035 representing NM_022362
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGGAGAGCCCGTCTCGAGCCACCGCCCATATCCCCTCCCACGGTCTCTAGTTCGCGTTATGGCCG
  CTGCCGCGGCTGTGGAGGCGGCGGCGCCTATGGGTGCCCTATGGGGCCTCGTGCACGACTTCGTCGTGGG
  TCAGCAAGAGGGCCCCGCTGACCAGGTGGCTGCAGATGTGAAATCTGGCAACTATACAGTGTTACAAGTT
  GTGGAAGCCCTTGGGTCCTCTCTAGAGAATCCAGAACCCCGAACTCGGGCACGAGCAATCCAGCTTTTGT
  CACAGGTGCTACTCCACTGTCACACCTTGCTCCTGGAGAAGGAAGTGGTACACCTGATACTGTTCTATGA
  GAACCGGCTGAAGGACCATCATCTTGTGATCCCATCTGTCCTGCAGGGTTTGAAGGCACTTAGCCTGTGT
  GTGGCCCTGCCCCCAGGGCTGGCTGTTTCTGTGCTTAAAGCCATCTTCCAGGAAGTGCATGTACAGTCCC
  TGCCACAGGTGGACCGACACACAGTCTACAATATCATCACCAATTTTATGCGAACCCGGGAAGAAGAGCT
  AAAGAGCCTAGGAGCTGACTTCACCTTTGGCTTCATCCAGGTGATGGATGGGGAAAAGGATCCCCGTAAT
  CTTCTGGTGGCCTTCCGCATCGTCCATGACCTCATCTCCAGGGACTATAGCCTGGGACCCTTTGTGGAGG
  AGTTGTTTGAAGTGACATCCTGTTATTTCCCTATCGATTTTACCCCTCCACCTAATGATCCCCATGGTAT
  CCAGAGAGAAGACCTCATCCTGAGTCTTCGCGCTGTGCTGGCTTCTACACCACGATTTGCTGAGTTTCTG
  CTGCCCCTGTTGATTGAGAAAGTGGATTCTGAGGTTCTGAGTGCCAAGTTGGATTCTCTACAGACTCTGA
  ATGCTTGCTGTGCTGTGTATGGACAGAAGGAACTGAAGGACTTCCTCCCCAGCCTTTGGGCTTCTATCCG
  CAGAGAGGTGTTCCAGACGGCAAGTGAGCGGGTGGAGGCAGAGGGCCTGGCGGCCCTCCACTCCCTGACT
  GCGTGTTTGTCTCGCTCTGTGCTGAGGGCTGATGCTGAGGACCTCCTTGACTCCTTCCTTAGCAACATTC
  TACAGGACTGCAGGCACCACCTGTGTGAACCGGACATGAAACTGGTGTGGCCTAGTGCCAAGCTGTTGCA
  GGCAGCTGCAGGTGCATCTGCCCGGGCCTGTGACTCTGTCACCAGCAATGTACTGCCTTTACTGCTGGAA
  CAGTTCCACAAGCACAGTCAGAGCAGCCAGCGGCGGACAATCCTTGAAATGCTCCTGGGTTTCTTGAAGC
  TGCAGCAGAAATGGAGCTATGAAGACAAAGATCAAAGGCCTCTGAATGGCTTCAAGGACCAGCTGTGCTC
  ACTGGTATTCATGGCTCTAACAGACCCCAGCACCCAGCTTCAGCTTGTTGGCATCCGTACACTGACAGTC
  TTGGGTGCCCAGCCAGATCTCCTATCTTATGAGGACTTGGAGCTGGCAGTGGGTCACCTGTACAGACTGA
  GCTTCCTGAAGGAGGATTCCCAGAGTTGCAGGGTGGCAGCACTGGAAGCATCAGGAACCCTGGCTGCTCT
  CTACCCTGTGGCCTTCAGCAGCCACCTCGTACCCAAGCTCGCTGAGGAGCTGCGTGTAGGGGAGTCAAAT
  TTGACTAACGGAGATGAGCCCACCCAATGCTCCCGGCATCTGTGCTGTCTGCAAGCCTTGTCAGCTGTAT
  CAACACATCCCAGCATCGTCAAGGAGACACTGCCTCTGCTGCTGCAGCATCTCTGGCAAGTGAACAGAGG
  GAATATGGTTGCACAATCCAGTGACGTTATTGCTGTCTGTCAGAGCCTCAGACAGATGGCAGAAAAATGT
  CAGCAGGACCCTGAGAGTTGCTGGTATTTCCACCAGACAGCTATACCTTGCCTGCTTGCCTTGGCTGTGC
  AGGCCTCTATGCCAGAGAAGGAGCCCTCAGTTCTGAGAAAAGTACTATTGGAGGATGAGGTGTTGGCTGC
  CATGGTGTCTGTCATTGGCACTGCTACAACCCACCTGAGCCCTGAGTTAGCTGCCCAGAGTGTGACACAC
  ATTGTGCCCCTCTTCTTGGATGGCAACGTGTCCTTTCTGCCTGAAAACAGCTTCCCGAGCAGATTCCAGC
  CATTCCAGGATGGCTCCTCAGGGCAGAGGCGGCTGATTGCACTGCTTATGGCCTTTGTCTGCTCCCTGCC
  TCGAAATGTGGAAATCCCTCAGCTGAACCAACTCATGCGGGAGCTTTTGGAACTGAGCTGCTGCCACAGC
  TGCCCCTTTTCTTCCACCGCTGCTGCCAAGTGCTTTGCAGGACTCCTCAACAAGCACCCTGCAGGGCAGC
  AGCTGGATGAATTCCTACAGCTAGCTGTGGACAAAGTGGAGGCTGGCCTGGGCTCTGGGCCCTGTCGTAG
  TCAGGCCTTCACTCTTCTTCTCTGGGTAACAAAGGCCCTAGTGCTCAGATACCATCCTCTCAGCTCCTGC
  CTTACAGCCCGGCTCATGGGCCTCCTGAGTGACCCAGAATTAGGTCCAGCAGCAGCTGATGGCTTCTCTC
  TGCTCATGTCTGACTGCACTGATGTGCTGACTCGTGCTGGCCATGCCGAAGTGCGGATCATGTTCCGCCA
  GCGGTTCTTCACAGATAATGTGCCTGCTTTGGTCCAGGGCTTCCATGCTGCTCCCCAAGATGTGAAGCCA
  AACTACTTGAAGGGTCTTTCTCATGTACTTAACAGGCTGCCCAAGCCTGTACTCTTGCCAGAGCTGCCCA
  CGCTTCTTTCCTTGCTGCTGGAGGCCCTGTCCTGCCCTGACTGTGTGGTGCAGCTCTCCACCCTCAGCTG
  CCTTCAGCCTCTTCTACTGGAAGCACCCCAAGTCATGAGTCTTCACGTGGACACCCTCGTCACCAAGTTT
  CTGAACCTCAGCTCTAGCCCTTCCATGGCTGTCCGGATCGCCGCACTGCAGTGCATGCATGCTCTCACTC
  GCCTGCCCACCCCTGTGCTGCTGCCGTACAAACCACAGGTGATTCGGGCCTTAGCCAAACCCCTGGATGA
  CAAGAAGAGACTGGTGCGCAAGGAAGCAGTGTCAGCCAGAGGGGAGTGGTTTCTGTTGGGGAGCCCTGGC
  AGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224035 representing NM_022362
  Red=Cloning site Green=Tags(s)
  
  MRGEPVSSHRPYPLPRSLVRVMAAAAAVEAAAPMGALWGLVHDFVVGQQEGPADQVAADVKSGNYTVLQV
  VEALGSSLENPEPRTRARAIQLLSQVLLHCHTLLLEKEVVHLILFYENRLKDHHLVIPSVLQGLKALSLC
  VALPPGLAVSVLKAIFQEVHVQSLPQVDRHTVYNIITNFMRTREEELKSLGADFTFGFIQVMDGEKDPRN
  LLVAFRIVHDLISRDYSLGPFVEELFEVTSCYFPIDFTPPPNDPHGIQREDLILSLRAVLASTPRFAEFL
  LPLLIEKVDSEVLSAKLDSLQTLNACCAVYGQKELKDFLPSLWASIRREVFQTASERVEAEGLAALHSLT
  ACLSRSVLRADAEDLLDSFLSNILQDCRHHLCEPDMKLVWPSAKLLQAAAGASARACDSVTSNVLPLLLE
  QFHKHSQSSQRRTILEMLLGFLKLQQKWSYEDKDQRPLNGFKDQLCSLVFMALTDPSTQLQLVGIRTLTV
  LGAQPDLLSYEDLELAVGHLYRLSFLKEDSQSCRVAALEASGTLAALYPVAFSSHLVPKLAEELRVGESN
  LTNGDEPTQCSRHLCCLQALSAVSTHPSIVKETLPLLLQHLWQVNRGNMVAQSSDVIAVCQSLRQMAEKC
  QQDPESCWYFHQTAIPCLLALAVQASMPEKEPSVLRKVLLEDEVLAAMVSVIGTATTHLSPELAAQSVTH
  IVPLFLDGNVSFLPENSFPSRFQPFQDGSSGQRRLIALLMAFVCSLPRNVEIPQLNQLMRELLELSCCHS
  CPFSSTAAAKCFAGLLNKHPAGQQLDEFLQLAVDKVEAGLGSGPCRSQAFTLLLWVTKALVLRYHPLSSC
  LTARLMGLLSDPELGPAAADGFSLLMSDCTDVLTRAGHAEVRIMFRQRFFTDNVPALVQGFHAAPQDVKP
  NYLKGLSHVLNRLPKPVLLPELPTLLSLLLEALSCPDCVVQLSTLSCLQPLLLEAPQVMSLHVDTLVTKF
  LNLSSSPSMAVRIAALQCMHALTRLPTPVLLPYKPQVIRALAKPLDDKKRLVRKEAVSARGEWFLLGSPG
  S
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022362
• ORF Size: 3153 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022362.3, NP_071757.3
• RefSeq Size: 3606
• RefSeq ORF: 3093
• Locus ID: 64210
• Protein Families: Druggable Genome, Transcription Factors
• MW: 113.1 kDa