PFTK1 (CDK14) (NM_012395) Human Tagged ORF Clone

CAT#: RC224426

CDK14 (Myc-DDK-tagged)-Human cyclin-dependent kinase 14 (CDK14)

Product Images

Western blot validation of overexpression lysate (Cat# LY415792) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224426 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CDK14 protein (Cat# TP324426). The protein was produced from HEK293T cells transfected with CDK14 cDNA clone (Cat# RC224426) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDK14
• Synonyms: PFTAIRE1; PFTK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224426 representing NM_012395
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACGGTTACTTTGGCTGCAATGCTGCTGCAGAGCCCGGTTACTCTGCCTTCGTGGGAACTCCACAGA
  TATGTGTCACAAAGATGTCTACACGGAACTGCCAGGGAATGGACTCAGTGATCAAACCCCTGGACACAAT
  TCCTGAGGATAAAAAAGTCAGAGTTCAGAGGACACAGAGCACTTTTGACCCATTTGAGAAACCAGCTAAT
  CAAGTAAAGAGGGTGCATTCTGAGAACAATGCTTGCATTAACTTTAAGACCTCCTCCACTGGCAAAGAGT
  CACCTAAAGTTAGGCGGCACTCCAGCCCCAGCTCGCCAACAAGTCCCAAATTTGGAAAAGCTGACTCATA
  TGAAAAGCTGGAAAAACTAGGGGAAGGATCTTATGCTACAGTATACAAAGGGAAAAGCAAGGTAAATGGG
  AAGTTGGTAGCTCTGAAGGTGATCAGGCTGCAGGAAGAAGAAGGGACACCTTTCACAGCTATCAGGGAAG
  CTTCTCTTTTAAAAGGACTAAAACATGCTAACATAGTGCTACTTCATGACATCATCCATACCAAGGAGAC
  GCTGACACTTGTGTTTGAATATGTGCACACTGATTTATGTCAGTACATGGACAAGCACCCTGGGGGGCTG
  CATCCAGATAATGTGAAGTTGTTTTTATTTCAGTTGCTGCGAGGTCTGTCTTACATCCACCAGCGTTATA
  TTTTGCACAGAGACCTGAAACCACAGAACCTTCTGATCAGTGACACGGGGGAGTTAAAGCTGGCAGATTT
  CGGTCTTGCAAGAGCAAAATCCGTCCCTAGCCACACATACTCCAACGAAGTGGTTACCTTGTGGTACAGA
  CCTCCAGATGTCCTTCTAGGCTCAACAGAATATTCCACCTGCCTTGACATGTGGGGAGTAGGTTGCATCT
  TTGTTGAAATGATCCAAGGAGTTGCTGCTTTTCCAGGAATGAAAGACATTCAGGATCAACTTGAACGAAT
  ATTTCTGGTTCTTGGAACACCAAATGAGGACACATGGCCTGGAGTTCATTCTTTACCACATTTTAAGCCA
  GAACGCTTTACCCTGTACAGCTCTAAAAACCTTAGACAAGCATGGAATAAGCTCAGCTATGTGAACCATG
  CAGAGGACCTGGCCTCCAAGCTCCTACAATGTTCCCCAAAGAACAGACTGTCGGCACAGGCTGCCTTGAG
  CCACGAGTATTTTAGTGACCTGCCGCCACGGCTATGGGAACTCACCGACATGTCTTCTATTTTTACTGTC
  CCAAATGTGAGATTGCAACCAGAAGCTGGAGAAAGCATGCGGGCCTTTGGGAAAAACAATAGTTATGGCA
  AAAGTCTATCAAACAGCAAGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224426 representing NM_012395
  Red=Cloning site Green=Tags(s)
  
  MHGYFGCNAAAEPGYSAFVGTPQICVTKMSTRNCQGMDSVIKPLDTIPEDKKVRVQRTQSTFDPFEKPAN
  QVKRVHSENNACINFKTSSTGKESPKVRRHSSPSSPTSPKFGKADSYEKLEKLGEGSYATVYKGKSKVNG
  KLVALKVIRLQEEEGTPFTAIREASLLKGLKHANIVLLHDIIHTKETLTLVFEYVHTDLCQYMDKHPGGL
  HPDNVKLFLFQLLRGLSYIHQRYILHRDLKPQNLLISDTGELKLADFGLARAKSVPSHTYSNEVVTLWYR
  PPDVLLGSTEYSTCLDMWGVGCIFVEMIQGVAAFPGMKDIQDQLERIFLVLGTPNEDTWPGVHSLPHFKP
  ERFTLYSSKNLRQAWNKLSYVNHAEDLASKLLQCSPKNRLSAQAALSHEYFSDLPPRLWELTDMSSIFTV
  PNVRLQPEAGESMRAFGKNNSYGKSLSNSKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012395
• ORF Size: 1353 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012395.1, NM_012395.2, NM_012395.3, NP_036527.1
• RefSeq Size: 4953 bp
• RefSeq ORF: 1356 bp
• Locus ID: 5218
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 50.5 kDa
• Gene Summary: PFTK1 is a member of the CDC2 (MIM 116940)-related protein kinase family (Yang and Chen, 2001 [PubMed 11313143]). [supplied by OMIM, Mar 2008]