CTNNA3 (NM_001127384) Human Tagged ORF Clone

CAT#: RC226241

CTNNA3 (Myc-DDK-tagged)-Human catenin (cadherin-associated protein), alpha 3 (CTNNA3), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY426767) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226241 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CTNNA3
• Synonyms: ARVD13; VR22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226241 representing NM_001127384
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCTGAAACACCAATCACATTGAATATCGATCCTCAGGATCTGCAGGTCCAAACATTCACCGTGG
  AGAAGCTACTGGAGCCTCTCATAATCCAGGTTACCACACTTGTAAACTGTCCCCAGAACCCTTCCAGCAG
  GAAAAAAGGACGTTCGAAAAGAGCCAGTGTCCTTCTAGCTTCTGTGGAGGAAGCAACTTGGAATTTATTA
  GACAAGGGAGAGAAGATTGCCCAGGAAGCTACAGTTTTAAAGGATGAGCTTACGGCTTCACTTGAGGAAG
  TTCGCAAAGAAAGTGAAGCTCTGAAAGTATCAGCTGAGAGATTTACAGATGACCCCTGTTTTCTCCCAAA
  AAGGGAGGCTGTGGTTCAAGCTGCCCGTGCCTTGCTGGCTGCGGTGACGAGACTCCTTATCCTTGCGGAC
  ATGATTGATGTCATGTGCCTCTTGCAACATGTGTCAGCTTTTCAAAGGACATTTGAGTCTCTCAAAAATG
  TTGCCAACAAATCTGACCTCCAGAAAACCTACCAGAAGCTTGGGAAGGAGCTGGAAAATTTGGATTATTT
  AGCCTTCAAACGTCAGCAGGACTTAAAATCTCCAAATCAGAGAGATGAAATTGCAGGAGCCCGAGCTTCA
  CTGAAGGAGAACTCTCCCCTCTTGCATTCAATTTGTTCAGCTTGTTTGGAGCATTCTGATGTTGCTTCCC
  TCAAAGCAAGCAAGGACACAGTTTGTGAAGAAATTCAGAATGCTCTCAATGTAATTTCAAATGCTTCACA
  AGGGATCCAGAATATGACAACCCCACCAGAACCTCAGGCAGCAACCCTGGGAAGTGCCCTTGATGAGCTG
  GAGAATTTAATTGTCCTGAATCCACTCACAGTAACTGAGGAGGAAATACGACCATCACTAGAGAAACGCC
  TTGAAGCCATTATCAGTGGGGCTGCTCTGCTGGCGGATTCTTCATGTACGAGGGACTTACACCGAGAGCG
  GATTATCGCAGAATGCAACGCCATTCGCCAGGCTCTTCAGGATCTGCTTTCAGAGTACATGAACAACGCT
  GGAAAAAAAGAAAGGAGTAATACCCTGAATATTGCTTTAGACAACATGTGTAAGAAGACAAGAGACCTTC
  GCAGACAGCTCCGCAAGGCTATTATAGATCATGTGTCAGACTCTTTCCTGGATACGACAGTCCCTCTTTT
  GGTTCTCATTGAAGCTGCTAAGAATGGCCGGGAAAAGGAAATAAAAGAATATGCTGCGATATTTCATGAA
  CACACCAGCAGGCTTGTAGAGGTGGCAAATCTTGCTTGTTCCATGTCAACAAATGAAGATGGAATTAAAA
  TTGTCAAAATTGCAGCCAATCATTTGGAAACCTTGTGTCCACAGATTATTAATGCTGCACTTGCTTTGGC
  TGCAAGACCCAAAAGTCAAGCGGTCAAAAACACCATGGAAATGTACAAGCGTACATGGGAGAATCATATA
  CATGTCCTCACTGAAGCCGTAGATGACATTACAAGCATTGATGACTTCCTTGCTGTATCTGAAAGCCATA
  TCTTGGAAGATGTCAACAAGTGTATCATAGCCTTAAGAGACCAGGATGCTGATAATTTAGACCGTGCTGC
  GGGTGCTATCAGAGGCCGGGCAGCAAGAGTTGCTCACATCGTCACGGGTGAAATGGACAGTTACGAGCCA
  GGGGCTTACACGGAAGGTGTAATGAGAAATGTTAACTTCCTTACAAGTACTGTAATTCCTGAATTTGTAA
  CACAAGTGAATGTTGCCTTGGAAGCCTTAAGCAAAAGCTCATTGAATGTGTTGGATGATAATCAATTTGT
  GGACATCTCAAAGAAGATCTATGATACAATTCATGATATCAGATGTTCAGTCATGATGATTCGGACCCCA
  GAGGAACTGGAGGATGTTTCTGACCTTGAAGAGGAACACGAGGTCCGCAGTCACACCAGCATTCAGACCG
  AAGGGAAAACTGATAGGGCTAAGATGACTCAACTGCCTGAGGCAGAAAAAGAAAAGATTGCTGAGCAAGT
  TGCTGATTTCAAGAAAGTAAAGAGTAAGCTGGATGCTGAGATTGAGATATGGGATGATACAAGCAACGAC
  ATCATTGTTCTGGCCAAGAACATGTGTATGATCATGATGGAGATGACAGACTTCACTAGGGGCAAAGGAC
  CACTAAAGCATACAACTGATGTGATCTATGCAGCGAAAATGATATCAGAATCAGGATCAAGGATGGATGT
  CCTTGCTCGGCAGATTGCTAATCAGTGCCCAGATCCATCTTGTAAACAGGACTTGTTGGCCTACCTGGAA
  CAGATTAAGTTCTACTCCCACCAACTGAAAATCTGCAGTCAAGTTAAAGCTGAGATCCAGAACCTGGGAG
  GAGAGCTCATCATGTCAGCTTTGGACAGTGTCACATCCCTGATCCAAGCAGCCAAAAATTTAATGAATGC
  TGTAGTGCAAACAGTGAAAATGTCTTACATTGCCTCAACCAAGATCATCCGAATCCAGAGTCCTGCTGGG
  CCCCGGCACCCAGTTGTGATGTGGAGAATGAAGGCTCCTGCAAAAAAACCCTTGATTAAAAGAGAGAAGC
  CAGAGGAAACGTGTGCAGCTGTCAGACGAGGCTCAGCAAAGAAAAAAATCCATCCATTGCAAGTCATGAG
  TGAATTTAGAGGAAGACAAATCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226241 representing NM_001127384
  Red=Cloning site Green=Tags(s)
  
  MSAETPITLNIDPQDLQVQTFTVEKLLEPLIIQVTTLVNCPQNPSSRKKGRSKRASVLLASVEEATWNLL
  DKGEKIAQEATVLKDELTASLEEVRKESEALKVSAERFTDDPCFLPKREAVVQAARALLAAVTRLLILAD
  MIDVMCLLQHVSAFQRTFESLKNVANKSDLQKTYQKLGKELENLDYLAFKRQQDLKSPNQRDEIAGARAS
  LKENSPLLHSICSACLEHSDVASLKASKDTVCEEIQNALNVISNASQGIQNMTTPPEPQAATLGSALDEL
  ENLIVLNPLTVTEEEIRPSLEKRLEAIISGAALLADSSCTRDLHRERIIAECNAIRQALQDLLSEYMNNA
  GKKERSNTLNIALDNMCKKTRDLRRQLRKAIIDHVSDSFLDTTVPLLVLIEAAKNGREKEIKEYAAIFHE
  HTSRLVEVANLACSMSTNEDGIKIVKIAANHLETLCPQIINAALALAARPKSQAVKNTMEMYKRTWENHI
  HVLTEAVDDITSIDDFLAVSESHILEDVNKCIIALRDQDADNLDRAAGAIRGRAARVAHIVTGEMDSYEP
  GAYTEGVMRNVNFLTSTVIPEFVTQVNVALEALSKSSLNVLDDNQFVDISKKIYDTIHDIRCSVMMIRTP
  EELEDVSDLEEEHEVRSHTSIQTEGKTDRAKMTQLPEAEKEKIAEQVADFKKVKSKLDAEIEIWDDTSND
  IIVLAKNMCMIMMEMTDFTRGKGPLKHTTDVIYAAKMISESGSRMDVLARQIANQCPDPSCKQDLLAYLE
  QIKFYSHQLKICSQVKAEIQNLGGELIMSALDSVTSLIQAAKNLMNAVVQTVKMSYIASTKIIRIQSPAG
  PRHPVVMWRMKAPAKKPLIKREKPEETCAAVRRGSAKKKIHPLQVMSEFRGRQIY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127384
• ORF Size: 2685 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001127384.1, NM_001127384.2, NP_001120856.1
• RefSeq ORF: 2688
• Locus ID: 29119
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Endometrial cancer, Leukocyte transendothelial migration, Pathways in cancer, Tight junction
• MW: 99.6 kDa
• Gene Summary: This gene encodes a protein that belongs to the vinculin/alpha-catenin family. The encoded protein plays a role in cell-cell adhesion in muscle cells. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia, familial 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]