DAGLB (NM_001142936) Human Tagged ORF Clone

CAT#: RC226682

DAGLB (Myc-DDK-tagged)-Human diacylglycerol lipase, beta (DAGLB), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY428303) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226682 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DAGLB
• Synonyms: DAGLBETA; KCCR13L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226682 representing NM_001142936
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGGATGGTACTCTTCGGCCGGCGCTGGGCCATCGCCAGCGACGACTTGGTCTTCCCAGGGTTCT
  TCGAGCTGGTCGTGCGAGTGCTGTGGTGGATTGGCATTCTGACGTTGTATCTCATGCACAGAGGAAAGCT
  GGACTGTGCTGGTGGAGCCTTGCTCAGCAGTTACTTGATCGTCCTCATGATTCTCCTGGCAGTTGTCATA
  TGTACTGTGTCAGCCATCATGTGTGTCAGCATGAGAGGAACGATTTGTAACCCTGGACCGCGGAAGTCTA
  TGTCTAAGCTGCTTTACATCCGCCTGGCGCTGTTTTTTCCAGAGATGGTCTGGGCCTCTCTGGGGGCTGC
  CTGGGTGGCAGATGGTGTTCAGTGCGACAGGACAGTTGTAAACGGCATCATCGCAACCGTCGTGGTCAGG
  ACACAGATCTGGTGCCCAGCGACATTGCGGCGGGCCTCGCCCTGCTTCATCAGCAACAGGACAATATCAG
  GAACAACCAAGAGCCTGCCCAGGTGGTCTGCCATGCCCCAGGGAGCTCCCAGCTGCAGAAGCAGAACCAC
  AGACTATGACTTGGTCGGAGGCGATCAGCTCAACTGTCACTTCGGCTCCATCCTGCACACCACAGGGCTG
  CAGTACAGGGACTTCATCCACGTCAGCTTCCATGACAAGGTTTACGAGCTGCCGTTTTTAGTGGCTCTGG
  ATCACAGGAAAGAGTCTGTTGTGGTCGCTGTGAGGGGGACCATGTCTCTGCAGGATGTCCTTACGGACCT
  GTCAGCGGAGAGTGAGGTGCTGGACGTGGAGTGTGAGGTGCAGGACCGCCTGGCACACAAGGGTATTTCT
  CAAGCTGCCAGATACGTTTACCAACGACTCATCAACGACGGGATTTTGAGCCAAGCCTTCAGCATTGCTC
  CTGAGTACCGGCTGGTCATAGTGGGCCACAGCCTCGGGGGCGGGGCGGCCGCCCTGCTGGCCACCATGCT
  CAGAGCCGCCTACCCGCAGGTCAGGTGCTACGCCTTCTCCCCACCCCGGGGGCTGTGGAGCAAAGCTCTG
  CAGGAATATTCTCAGAGCTTCATCGTGTCACTCGTCCTGGGGAAGGATGTGATTCCCAGGCTCAGTGTGA
  CCAACTTGGAAGATCTGAAGAGAAGAATCTTGCGAGTGGTCGCGCACTGCAATAAACCCAAGTACAAGAT
  CTTGCTGCACGGTTTGTGGTACGAACTGTTTGGAGGAAACCCCAACAACTTGCCCACGGAGCTGGACGGG
  GGCGACCAGGAAGTCCTGACACAGCCTCTTCTGGGGGAGCAGAGCCTACTGACGCGCTGGTCCCCGGCCT
  ACAGCTTCTCCAGCGACTCCCCACTGGACTCTTCTCCCAAGTACCCCCCTCTCTACCCTCCCGGCAGGAT
  CATCCACCTGCAGGAGGAGGGCGCCTCGGGGCGGTTTGGCTGCTGCTCTGCTGCTCACTATAGCGCCAAG
  TGGTCACACGAAGCGGAATTCAGCAAAATACTCATAGGTCCGAAGATGCTCACCGACCACATGCCAGACA
  TCCTGATGCGGGCCTTGGACAGCGTGGTCTCCGACAGAGCGGCCTGCGTCTCCTGTCCAGCACAAGGGGT
  CTCCAGTGTGGACGTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226682 representing NM_001142936
  Red=Cloning site Green=Tags(s)
  
  MPGMVLFGRRWAIASDDLVFPGFFELVVRVLWWIGILTLYLMHRGKLDCAGGALLSSYLIVLMILLAVVI
  CTVSAIMCVSMRGTICNPGPRKSMSKLLYIRLALFFPEMVWASLGAAWVADGVQCDRTVVNGIIATVVVR
  TQIWCPATLRRASPCFISNRTISGTTKSLPRWSAMPQGAPSCRSRTTDYDLVGGDQLNCHFGSILHTTGL
  QYRDFIHVSFHDKVYELPFLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDVECEVQDRLAHKGIS
  QAARYVYQRLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATMLRAAYPQVRCYAFSPPRGLWSKAL
  QEYSQSFIVSLVLGKDVIPRLSVTNLEDLKRRILRVVAHCNKPKYKILLHGLWYELFGGNPNNLPTELDG
  GDQEVLTQPLLGEQSLLTRWSPAYSFSSDSPLDSSPKYPPLYPPGRIIHLQEEGASGRFGCCSAAHYSAK
  WSHEAEFSKILIGPKMLTDHMPDILMRALDSVVSDRAACVSCPAQGVSSVDVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142936
• ORF Size: 1629 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142936.1, NP_001136408.1
• RefSeq ORF: 1632
• Locus ID: 221955
• Protein Families: Transmembrane
• MW: 59.7 kDa