AGXT2L1 (ETNPPL) (NM_001146627) Human Tagged ORF Clone

CAT#: RC228353

AGXT2L1 (Myc-DDK-tagged)-Human alanine-glyoxylate aminotransferase 2-like 1 (AGXT2L1), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY431381) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228353 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ETNPPL
• Synonyms: AGXT2L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228353 representing NM_001146627
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACACTGTCACCCAGGAGTGGTCAAAGCTGCCCTGAAACAGATGGAACTGCTAAATACAAATTCTC
  GATTCCTCCACGACAACATTGTTGAGTATGCCAAACGCCTTTCAGCAACTCTGCCGGAGAAACTCTCTGT
  TTGTTATTTTACAAATTCAGGATCCGAAGCCAACGACTTAGCCTTACGCCTGGCTCGGCAGTTCAGAGGC
  CACCAGGATGTGATCACTCTTGACCATGCTTACCATGGTCACCTATCATCCTTAATTGAGATTAGCCCAT
  ATAAGTTTCAGAAAGGAAAAGATGTCAAAAAAGAATTTGTACATGTGGCACCAACTCCAGATACTTACAG
  AGGAAAATATAGAGAAGACCATGCAGACTCAGCCAGTGCTTATGCAGATGAAGTGAAGAAAATCATTGAA
  GATGCTCATAACAGTGGAAGGAAGATTGCTGCCTTTATTGCTGAATCCATGCAGAGTTGTGGCGGACAAA
  TAATTCCTCCAGCAGGCTACTTCCAGAAAGTGGCAGAATATGTACACGGTGCAGGGGGTGTGTTTATAGC
  TGATGAAGTTCAAGTGGGCTTTGGCAGAGTTGGGAAACATTTCTGGAGCTTCCAGATGTATGGTGAAGAC
  TTTGTTCCAGACATCGTCACAATGGGAAAACCGATGGGCAACGGCCACCCGGTGGCATGTGTGGTAACAA
  CCAAAGAAATTGCAGAAGCCTTCAGCAGCTCTGGGATGGAATATTTTAATACGTATGGAGGAAATCCAGT
  ATCTTGTGCTGTTGGTTTGGCTGTCCTGGATATAATTGAAAATGAAGACCTTCAAGGAAATGCCAAGAGA
  GTAGGGAATTATCTCACTGAGTTACTGAAAAAACAGAAGGCTAAACACACTTTGATAGGAGATATTAGGG
  GCATTGGCCTTTTTATTGGAATTGATTTAGTGAAGGACCATCTGAAAAGGACCCCTGCCACAGCTGAAGC
  TCAGCACATCATCTACAAGATGAAAGAAAAACGAGTGCTTCTCAGTGCCGATGGACCTCATAGAAATGTA
  CTTAAAATAAAACCACCTATGTGCTTCACTGAAGAAGATGCAAAGTTCATGGTGGACCAACTTGATAGGA
  TTCTAACAGTTTTAGAAGAAGCTATGGGAACCAAAACCGAAAGTGTGACCTCTGAGAATACTCCATGCAA
  AACAAAGATGCTGAAAGAAGCCCACATAGAACTGCTTAGGGACAGCACCACTGACTCCAAAGAAAATCCC
  AGCAGAAAGAGAAATGGAATGTGCACGGATACACATTCACTGCTCAGTAAGAGGCTCAAGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228353 representing NM_001146627
  Red=Cloning site Green=Tags(s)
  
  MGHCHPGVVKAALKQMELLNTNSRFLHDNIVEYAKRLSATLPEKLSVCYFTNSGSEANDLALRLARQFRG
  HQDVITLDHAYHGHLSSLIEISPYKFQKGKDVKKEFVHVAPTPDTYRGKYREDHADSASAYADEVKKIIE
  DAHNSGRKIAAFIAESMQSCGGQIIPPAGYFQKVAEYVHGAGGVFIADEVQVGFGRVGKHFWSFQMYGED
  FVPDIVTMGKPMGNGHPVACVVTTKEIAEAFSSSGMEYFNTYGGNPVSCAVGLAVLDIIENEDLQGNAKR
  VGNYLTELLKKQKAKHTLIGDIRGIGLFIGIDLVKDHLKRTPATAEAQHIIYKMKEKRVLLSADGPHRNV
  LKIKPPMCFTEEDAKFMVDQLDRILTVLEEAMGTKTESVTSENTPCKTKMLKEAHIELLRDSTTDSKENP
  SRKRNGMCTDTHSLLSKRLKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146627
• ORF Size: 1323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001146627.1, NP_001140099.1
• RefSeq ORF: 1326
• Locus ID: 64850
• Protein Families: Druggable Genome
• MW: 48.8 kDa