AGXT2L1 (ETNPPL) (NM_001146590) Human Tagged ORF Clone

CAT#: RC228408

AGXT2L1 (Myc-DDK-tagged)-Human alanine-glyoxylate aminotransferase 2-like 1 (AGXT2L1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY431436) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228408 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ETNPPL
• Synonyms: AGXT2L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228408 representing NM_001146590
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCGAGCTGTACAGTAAGCGGGACACTCTGGGGCTGAGGAAGAAGCACATCGGGCCCTCATGCAAAG
  TTTTCTTTGCATCGGATCCCATCAAAATAGTGAGAGCCCAGAGGCAGTACATGTTTGATGAGAACGGTGA
  ACAGTACTTGGACTGCATCAACAATGTTGCCCATGGAGTGGTCAAAGCTGCCCTGAAACAGATGGAACTG
  CTAAATACAAATTCTCGATTCCTCCACGACAACATTGTTGAGTATGCCAAACGCCTTTCAGCAACTCTGC
  CGGAGAAACTCTCTGTTTGTTATTTTACAAATTCAGGATCCGAAGCCAACGACTTAGCCTTACGCCTGGC
  TCGGCAGTTCAGAGGCCACCAGGATGTGATCACTCTTGACCATGCTTACCATGGTCACCTATCATCCTTA
  ATTGAGATTAGCCCATATAAGTTTCAGAAAGGAAAAGATGTCAAAAAAGAATTTGTACATGTGGCACCAA
  CTCCAGATACTTACAGAGGAAAATATAGAGAAGACCATGCAGACTCAGCCAGTGCTTATGCAGATGAAGT
  GAAGAAAATCATTGAAGATGCTCATAACAGTGGAAGGAAGATTGCTGCCTTTATTGCTGAATCCATGCAG
  AGTTGTGGCGGACAAATAATTCCTCCAGCAGGCTACTTCCAGAAAGTGGCAGAATATGTACACGGTGCAG
  GGGGTGTGTTTATAGCTGATGAAGTTCAAGTGGGCTTTGGCAGAGTTGGGAAACATTTCTGGAGCTTCCA
  GATGTATGGTGAAGACTTTGTTCCAGACATCGTCACAATGGGAAAACCGATGGGCAACGGCCACCCGGTG
  GCATGTGTGGTAACAACCAAAGAAATTGCAGAAGCCTTCAGCAGCTCTGGGATGGAATATTTTAATACGT
  ATGGAGGAAATCCAGTATCTTGTGCTGTTGGTTTGGCTGTCCTGGATATAATTGAAAATGAAGACCTTCA
  AGGAAATGCCAAGAGAGTAGGGAATTATCTCACTGAGTTACTGAAAAAACAGAAGGCTAAACACACTTTG
  ATAGGAGATATTAGGGGCATTGGCCTTTTTATTGGAATTGATTTAGTGAAGGACCATCTGAAAAGGACCC
  CTGCCACAGCTGAAGCTCAGCACATCATCTACAAGATGAAAGAAAAACGAGTGCTTCTCAGTGCCGATGG
  ACCTCATAGAAATGTACTTAAAATAAAACCACCTATGTGCTTCACTGAAGAAGATGCAAAGTTCATGGTG
  GACCAACTTGATAGGATTCTAACAGTTTTAGAAGAAGCTATGGGAACCAAAACCGAAAGTGTGACCTCTG
  AGAATACTCCATGCAAAACAAAGATGCTGAAAGAAGCCCACATAGAACTGCTTAGGGACAGCACCACTGA
  CTCCAAAGAAAATCCCAGCAGAAAGAGAAATGGAATGTGCACGGATACACATTCACTGCTCAGTAAGAGG
  CTCAAGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228408 representing NM_001146590
  Red=Cloning site Green=Tags(s)
  
  MCELYSKRDTLGLRKKHIGPSCKVFFASDPIKIVRAQRQYMFDENGEQYLDCINNVAHGVVKAALKQMEL
  LNTNSRFLHDNIVEYAKRLSATLPEKLSVCYFTNSGSEANDLALRLARQFRGHQDVITLDHAYHGHLSSL
  IEISPYKFQKGKDVKKEFVHVAPTPDTYRGKYREDHADSASAYADEVKKIIEDAHNSGRKIAAFIAESMQ
  SCGGQIIPPAGYFQKVAEYVHGAGGVFIADEVQVGFGRVGKHFWSFQMYGEDFVPDIVTMGKPMGNGHPV
  ACVVTTKEIAEAFSSSGMEYFNTYGGNPVSCAVGLAVLDIIENEDLQGNAKRVGNYLTELLKKQKAKHTL
  IGDIRGIGLFIGIDLVKDHLKRTPATAEAQHIIYKMKEKRVLLSADGPHRNVLKIKPPMCFTEEDAKFMV
  DQLDRILTVLEEAMGTKTESVTSENTPCKTKMLKEAHIELLRDSTTDSKENPSRKRNGMCTDTHSLLSKR
  LKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146590
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001146590.1, NP_001140062.1
• RefSeq ORF: 1482
• Locus ID: 64850
• Protein Families: Druggable Genome
• MW: 54.9 kDa