DACT3 (NM_145056) Human Tagged ORF Clone

CAT#: RC229315

DACT3 (Myc-DDK-tagged)-Human dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) (DACT3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DACT3
• Synonyms: DAPPER3; RRR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229315 representing NM_145056
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCGGGCCTTCTCGTTCCCGGTGAGCCCTGAGCGGGGCCGGCTGCGGGGCTGGCTGGAGGGTAGCC
  TGGCCGGGCTCTGCGAGTTACATTGGCTCCGGGAGAGGCAGGAGTACCGCGTGCAGCAGGCGCTGCGGCT
  GGCCCAGCCCGGAATGGGGGGCGCCGAGGCCGAGGACGAGGAGGACGCCGATGAGGATGAAGATGCGGCG
  GCGGCGCGCCGGGCCGCAGCGGCCCTGGAGGAGCAGCTGGAGGCCCTGCCTGGTCTCGTCTGGGACCTGG
  GACAGCAGCTGGGAGACCTGAGCCTGGAGTCTGGGGGCCTGGAACAGGAGAGCGGGCGTAGCTCGGGCTT
  CTATGAAGATCCCAGCTCTACAGGAGGTCCAGATTCACCACCCTCAACCTTCTGTGGGGACAGTGGCTTC
  TCTGGATCCAGCTCCTATGGTCGCCTGGGTCCCTCTGAGCCCCGGGGCATCTATGCCAGTGAGAGGCCCA
  AGTCCCTAGGAGACGCCAGTCCCAGCGCTCCGGAGGTGGTGGGCGCGCGGGCAGCGGTGCCGCGGTCCTT
  CTCAGCGCCCTACCCGACGGCAGGGGGGTCCGCCGGCCCGGAGGCCTGCTCCTCGGCGGAGCGGCGGGCC
  CGCGCCGGGCCCTTTCTGACGCCCAGCCCCCTGCACGCCGTGGCGATGCGCAGCCCGCGGCCCTGCGGCC
  GCCCTCCCACCGACTCGCCCGACGCGGGGGGCGCAGGGCGGCCCCTGGACGGCTACATCTCGGCGCTCCT
  GCGCAGGCGCCGCCGCCGGGGGGCGGGCCAGCCCCGGACCAGTCCCGGGGGCGCGGACGGCGGCCCGCGG
  CGCCAGAACAGCGTGCGCCAGCGGCCGCCCGACGCGTCTCCGTCCCCCGGCAGCGCGCGACCCGCGCGGG
  AGCCCTCGTTGGAGCGCGTCGGGGGCCACCCCACCAGCCCTGCCGCCTTGAGCCGCGCCTGGGCGTCGTC
  GTGGGAGTCGGAGGCGGCACCCGAGCCCGCTGCGCCGCCCGCCGCCCCCTCACCCCCCGACAGCCCGGCT
  GAGGGCCGCTTGGTGAAGGCGCAGTACATCCCGGGCGCGCAGGCGGCCACCCGAGGCCTCCCTGGCCGCG
  CCGCCCGCCGCAAACCGCCGCCACTGACCCGCGGCCGCAGCGTGGAGCAGTCACCACCCCGGGAGCGTCC
  CCGGGCCGCCGGCCGCCGTGGACGCATGGCCGAGGCTTCGGGCCGCCGCGGCTCGCCCAGGGCCCGCAAG
  GCCTCGCGCTCCCAGTCTGAGACCAGCCTGCTGGGCCGCGCCTCCGCGGTCCCTTCGGGGCCCCCTAAGT
  ACCCCACGGCGGAGCGGGAAGAGCCTCGGCCTCCACGGCCACGCCGCGGCCCAGCGCCCACGCTGGCGGC
  CCAGGCCGCAGGGTCCTGCCGTCGCTGGCGCTCCACTGCGGAGATCGACGCTGCCGATGGGCGCCGCGTG
  CGGCCCCGAGCCCCTGCGGCGCGTGTTCCCGGCCCCGGCCCGTCCCCGTCAGCTCCCCAGCGTCGTCTGC
  TTTACGGCTGCGCGGGCAGCGACTCCGAGTGCTCGGCTGGGCGCCTGGGGCCCCTGGGACGCCGGGGGCC
  TGCGGGAGGCGTCGGCGGGGGTTACGGGGAGAGCGAATCGAGCGCCAGCGAGGGAGAATCGCCTGCCTTC
  AGCTCTGCCTCCAGCGACTCAGACGGCAGCGGTGGCCTCGTGTGGCCGCAGCAGCTGGTGGCGGCCACCG
  CGGCCTCTGGGGGTGGAGCAGGTGCAGGGGCGCCCGCAGGCCCCGCCAAAGTCTTCGTGAAAATCAAAGC
  TTCCCACGCGCTCAAGAAAAAGATACTGCGTTTCCGTTCGGGTTCTCTCAAGGTCATGACTACAGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229315 representing NM_145056
  Red=Cloning site Green=Tags(s)
  
  MIRAFSFPVSPERGRLRGWLEGSLAGLCELHWLRERQEYRVQQALRLAQPGMGGAEAEDEEDADEDEDAA
  AARRAAAALEEQLEALPGLVWDLGQQLGDLSLESGGLEQESGRSSGFYEDPSSTGGPDSPPSTFCGDSGF
  SGSSSYGRLGPSEPRGIYASERPKSLGDASPSAPEVVGARAAVPRSFSAPYPTAGGSAGPEACSSAERRA
  RAGPFLTPSPLHAVAMRSPRPCGRPPTDSPDAGGAGRPLDGYISALLRRRRRRGAGQPRTSPGGADGGPR
  RQNSVRQRPPDASPSPGSARPAREPSLERVGGHPTSPAALSRAWASSWESEAAPEPAAPPAAPSPPDSPA
  EGRLVKAQYIPGAQAATRGLPGRAARRKPPPLTRGRSVEQSPPRERPRAAGRRGRMAEASGRRGSPRARK
  ASRSQSETSLLGRASAVPSGPPKYPTAEREEPRPPRPRRGPAPTLAAQAAGSCRRWRSTAEIDAADGRRV
  RPRAPAARVPGPGPSPSAPQRRLLYGCAGSDSECSAGRLGPLGRRGPAGGVGGGYGESESSASEGESPAF
  SSASSDSDGSGGLVWPQQLVAATAASGGGAGAGAPAGPAKVFVKIKASHALKKKILRFRSGSLKVMTTV
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145056
• ORF Size: 1887 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145056.2, NP_659493.2
• RefSeq ORF: 1890 bp
• Locus ID: 147906
• Cytogenetics: 19q13.32
• MW: 64.8 kDa