ETFDH (NM_001281737) Human Tagged ORF Clone

CAT#: RC238932

ETFDH (myc-DDK-tagged) - Human electron-transferring-flavoprotein dehydrogenase (ETFDH), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ETFDH
• Synonyms: ETFQO; MADD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238932 representing NM_001281737
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGTGCCGCTAGCCAAGCTGTCCTGCCTGGGAGTGAACATGGAAAGGTTTGCAGAAGAAGCAGATG
  TTGTAATAGTTGGTGCAGGCCCTGCAGGGCTCTCTGCAGCTGTTCGTCTAAAACAGTTGGCTGTGGCACA
  TGAAAAGGACATCCGTGTGTGTCTAGTGGAGAAAGCTGCCCAGATAGGAGCTCATACTCTCTCAGGGGCT
  TGCCTTGATCCAGGTGCTTTTAAAGAACTCTTCCCAGACTGGAAAGAGAAGGGGGCTCCACTTAACACTC
  CTGTAACAGAAGACAGATTTGGAATTTTAACAGAGAAATACAGAATTCCTGTGCCAATTCTTCCAGGGCT
  TCCAATGAATAATCATGGCAATTACATTGTACGCTTGGGACATTTAGTGAGCTGGATGGGCGAACAAGCA
  GAAGCCCTTGGTGTTGAAGTATACCCTGGTTATGCAGCTGCTGAGGTCCTTTTTCATGATGATGGTAGTG
  TAAAAGGAATTGCCACTAACGATGTAGGGATACAAAAGGATGGTGCACCAAAGGCAACATTTGAGAGAGG
  ACTGGAACTACATGCTAAAGTCACAATTTTTGCAGAAGGTTGCCATGGACATCTAGCCAAGCAACTATAT
  AAGAAGTTTGATTTGAGAGCAAATTGTGAACCTCAAACCTACGGGATTGGACTGAAGGAGTTATGGGTTA
  TTGATGAAAAGAACTGGAAACCTGGGAGAGTAGATCACACTGTTGGTTGGCCCTTGGACAGACATACCTA
  TGGAGGATCTTTCCTCTATCATTTGAATGAAGGTGAACCCCTAGTAGCTCTTGGTCTTGTGGTTGGTCTA
  GACTATCAGAATCCATACCTGAGTCCATTTAGAGAGTTCCAAAGGTGGAAACACCATCCTAGCATTCGGC
  CAACCTTGGAAGGTGGAAAAAGGATTGCATACGGAGCCAGAGCTCTCAATGAAGGTGGCTTTCAGTCTAT
  ACCAAAACTCACCTTTCCTGGTGGTTTACTAATTGGTTGTAGTCCTGGTTTTATGAATGTTCCCAAGATC
  AAAGGTACTCACACAGCAATGAAAAGTGGAATTTTAGCAGCAGAATCTATTTTTAATCAACTAACTAGTG
  AAAATCTCCAATCAAAGACAATAGGACTCCATGTAACTGAATATGAGGACAATTTGAAGAACTCATGGGT
  ATGGAAAGAGCTATATTCTGTTAGAAATATAAGACCGTCCTGCCACGGAGTACTGGGTGTATATGGAGGG
  ATGATTTACACTGGAATCTTTTACTGGATATTGAGAGGAATGGAGCCGTGGACTCTGAAACATAAAGGTT
  CTGACTTTGAACGGCTCAAGCCAGCCAAGGATTGCACACCTATTGAGTATCCAAAACCCGATGGACAGAT
  CAGTTTTGACCTCTTGTCATCTGTGGCTCTGAGTGGTACTAATCATGAACATGACCAGCCGGCACACTTA
  ACCTTAAGGGATGACAGTATACCTGTAAATAGAAATCTGTCGATATATGATGGGCCCGAGCAGCGATTCT
  GTCCTGCAGGAGTTTATGAATTTGTACCTGTGGAACAAGGTGATGGATTTCGGTTACAGATAAATGCTCA
  GAACTGTGTACATTGTAAAACATGTGATATTAAAGATCCAAGTCAGAATATTAACTGGGTGGTACCTGAA
  GGTGGAGGAGGACCTGCTTACAATGGAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238932 representing NM_001281737
  Red=Cloning site Green=Tags(s)
  
  MLVPLAKLSCLGVNMERFAEEADVVIVGAGPAGLSAAVRLKQLAVAHEKDIRVCLVEKAAQIGAHTLSGA
  CLDPGAFKELFPDWKEKGAPLNTPVTEDRFGILTEKYRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQA
  EALGVEVYPGYAAAEVLFHDDGSVKGIATNDVGIQKDGAPKATFERGLELHAKVTIFAEGCHGHLAKQLY
  KKFDLRANCEPQTYGIGLKELWVIDEKNWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVALGLVVGL
  DYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKI
  KGTHTAMKSGILAAESIFNQLTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGG
  MIYTGIFYWILRGMEPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHL
  TLRDDSIPVNRNLSIYDGPEQRFCPAGVYEFVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPE
  GGGGPAYNGM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001281737
• ORF Size: 1710 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281737.1, NP_001268666.1
• RefSeq Size: 2255 bp
• RefSeq ORF: 1713 bp
• Locus ID: 2110
• Cytogenetics: 4q32.1
• MW: 63.3 kDa
• Gene Summary: 'This gene encodes a component of the electron-transfer system in mitochondria and is essential for electron transfer from a number of mitochondrial flavin-containing dehydrogenases to the main respiratory chain. Mutations in this gene are associated with glutaric acidemia. Alternatively spliced transcript variants that encode distinct isoforms have been observed. [provided by RefSeq, Aug 2013]'