USP8 (NM_001283049) Human Tagged ORF Clone

CAT#: RC239882

USP8 (myc-DDK-tagged) - Human ubiquitin specific peptidase 8 (USP8), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP8
• Synonyms: HumORF8; PITA4; SPG59; UBPY
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239882 representing NM_001283049
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGCTGTGGCTTCAGTTCCTAAAGAACTCTACCTCAGTTCTTCACTAAAAGACCTTAATAAGAAGA
  CAGAAGTTAAACCAGAGAAAATAAGCACTAAGAGATATGAAGAAGCTGAAGTCCGGAAAAAACTTGAGGA
  AAAAGACAGGCAGGAGGAAGCACAGCGGCTACAACAAAAAAGGCAGGAAACAGGAAGAGAGGATGGTGGC
  ACATTGGCTAAAGGCTCTTTGGAGAATGTTTTGGATTCCAAAGACAAAACCCAAAAGAGCAATGGTGAAA
  AGAATGAAAAATGTGAGACCAAAGAGAAAGGAGCAATCACAGCAAAGGAACTATACACAATGATGACGGA
  TAAAAACATCAGCTTGATTATAATGGATGCTCGAAGAATGCAGGATTATCAGGATTCCTGTATTTTACAT
  TCTCTCAGTGTTCCTGAAGAAGCCATCAGTCCAGGAGTCACTGCTAGTTGGATTGAAGCACACCTGCCAG
  ATGATTCTAAAGACACATGGAAGAAGAGGGGGAATGTGGAGTATGTGGTACTTCTTGACTGGTTTAGTTC
  TGCCAAAGATTTACAGATTGGAACAACTCTCCGGAGTCTGAAAGATGCACTTTTCAAGTGGGAAAGTAAA
  ACTGTCCTGCGCAATGAGCCTTTGGTTTTAGAGGGAGGCTATGAAAACTGGCTCCTTTGTTATCCCCAGT
  ATACAACAAATGCTAAGGTCACTCCACCCCCACGACGCCAGAATGAAGAGGTGTCTATCTCATTGGATTT
  TACTTATCCCTCATTGGAAGAATCAATTCCTTCTAAACCTGCTGCCCAGACGCCACCTGCATCTATAGAA
  GTAGATGAAAATATAGAATTGATAAGTGGTCAAAATGAGAGAATGGGACCACTGAATATATCAACTCCAG
  TTGAACCAGTTGCTGCTTCTAAATCTGATGTTTCACCCATAATTCAGCCAGTGCCTAGTATAAAGAATGT
  TCCACAGATTGATCGTACTAAAAAACCAGCAGTCAAATTGCCTGAAGAGCATAGAATAAAATCTGAAAGT
  ACAAACCATGAGCAACAATCTCCTCAGAGTGGAAAAGTTATTCCTGATCGTTCCACCAAGCCAGTAGTTT
  TTTCTCCAACTCTCATGTTAACAGATGAAGAAAAGGCTCGTATTCATGCAGAAACTGCTCTTCTAATGGA
  AAAAAACAAACAAGAAAAAGAACTTCGGGAAAGGCAGCAAGAGGAACAGAAAGAGAAACTGAGGAAGGAA
  GAACAAGAACAAAAAGCCAAAAAGAAACAAGAAGCTGAAGAAAATGAAATTACAGAGAAGCAACAAAAAG
  CAAAAGAAGAAATGGAGAAGAAAGAAAGTGAACAGGCCAAGAAAGAAGATAAAGAAACCTCAGCAAAGAG
  GGGCAAAGAAATAACAGGAGTAAAAAGACAAAGTAAAAGTGAACATGAAACTTCTGATGCCAAGAAATCT
  GTAGAAGATAGGGGGAAAAGGTGTCCAACCCCAGAAATACAGAAAAAGTCAACAGGAGATGTGCCCCATA
  CATCTGTGACAGGGGATTCAGGTTCAGGCAAGGCTCAACGAGAACCTTTGACAAGAGCACGAAGTGAAGA
  AATGGGGAGGATCGTACCAGGACTGCCTTCAGGCTGGGCCAAGTTTCTTGACCCAATCACTGGAACCTTT
  CGTTATTATCATTCACCCACCAACACTGTTCATATGTACCCACCGGAAATGGCTCCTTCATCTGCACCTC
  CTTCCACCCCTCCAACTCATAAAGCCAAGCCACAGATTCCTGCTGAGCGGGATAGGGAACCTTCCAAACT
  GAAGCGCTCCTACTCCTCCCCAGATATAACCCAGGCTATTCAAGAGGAAGAGAAGAGGAAGCCAACAGTA
  ACTCCAACAGTTAATCGGGAAAACAAGCCAACATGTTATCCTAAAGCTGAGATCTCAAGGCTTTCTGCTT
  CTCAGATTCGGAACCTCAATCCTGTTTTTGGAGGTTCTGGACCAGCTCTTACTGGACTTCGTAACTTAGG
  AAATACTTGTTATATGAACTCAATATTGCAGTGCCTATGTAACGCTCCACATTTGGCTGATTATTTCAAC
  CGAAACTGTTATCAGGATGATATTAACAGGTCAAATTTGTTGGGGCATAAAGGTGAAGTGGCAGAAGAAT
  TTGGTATAATCATGAAAGCCCTGTGGACAGGACAGTATAGATATATCAGTCCAAAGGACTTTAAAATCAC
  CATTGGGAAGATCAATGACCAGTTTGCAGGATACAGTCAGCAAGATTCACAAGAATTGCTTCTGTTCCTA
  ATGGATGGTCTCCATGAAGATCTAAATAAAGCTGATAATCGGAAGAGATATAAAGAAGAAAATAATGATC
  ATCTCGATGACTTTAAAGCTGCAGAACATGCCTGGCAGAAACACAAGCAGCTCAATGAGTCTATTATTGT
  TGCACTTTTTCAGGGTCAATTCAAATCTACAGTACAGTGCCTCACATGTCACAAAAAGTCTAGGACATTT
  GAGGCCTTCATGTATTTGTCTCTACCACTAGCATCCACAAGTAAATGTACATTACAGGATTGCCTTAGAT
  TATTTTCCAAAGAAGAAAAACTCACAGATAACAACAGATTTTACTGCAGTCATTGCAGAGCTCGACGGGA
  TTCTCTAAAAAAGATAGAAATCTGGAAGTTACCACCTGTGCTTTTAGTGCATCTGAAACGTTTTTCCTAC
  GATGGCAGGTGGAAACAAAAATTACAGACATCTGTGGACTTCCCGTTAGAAAATCTTGACTTGTCACAGT
  ATGTTATTGGTCCAAAGAACAATTTGAAGAAATATAATTTGTTTTCTGTTTCAAATCACTACGGTGGGCT
  GGATGGAGGCCACTACACAGCCTATTGTAAAAATGCAGCAAGACAACGGTGGTTTAAGTTTGATGATCAT
  GAAGTTTCTGATATCTCCGTTTCTTCTGTGAAATCTTCAGCAGCTTATATCCTCTTTTATACTTCATTGG
  GACCACGAGTAACTGATGTAGCCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239882 representing NM_001283049
  Red=Cloning site Green=Tags(s)
  
  MPAVASVPKELYLSSSLKDLNKKTEVKPEKISTKRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGG
  TLAKGSLENVLDSKDKTQKSNGEKNEKCETKEKGAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILH
  SLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNVEYVVLLDWFSSAKDLQIGTTLRSLKDALFKWESK
  TVLRNEPLVLEGGYENWLLCYPQYTTNAKVTPPPRRQNEEVSISLDFTYPSLEESIPSKPAAQTPPASIE
  VDENIELISGQNERMGPLNISTPVEPVAASKSDVSPIIQPVPSIKNVPQIDRTKKPAVKLPEEHRIKSES
  TNHEQQSPQSGKVIPDRSTKPVVFSPTLMLTDEEKARIHAETALLMEKNKQEKELRERQQEEQKEKLRKE
  EQEQKAKKKQEAEENEITEKQQKAKEEMEKKESEQAKKEDKETSAKRGKEITGVKRQSKSEHETSDAKKS
  VEDRGKRCPTPEIQKKSTGDVPHTSVTGDSGSGKAQREPLTRARSEEMGRIVPGLPSGWAKFLDPITGTF
  RYYHSPTNTVHMYPPEMAPSSAPPSTPPTHKAKPQIPAERDREPSKLKRSYSSPDITQAIQEEEKRKPTV
  TPTVNRENKPTCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFN
  RNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKITIGKINDQFAGYSQQDSQELLLFL
  MDGLHEDLNKADNRKRYKEENNDHLDDFKAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCHKKSRTF
  EAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSY
  DGRWKQKLQTSVDFPLENLDLSQYVIGPKNNLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDH
  EVSDISVSSVKSSAAYILFYTSLGPRVTDVAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001283049
• ORF Size: 3036 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001283049.1, NP_001269978.1
• RefSeq Size: 5250
• RefSeq ORF: 3039
• Locus ID: 9101
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Endocytosis
• MW: 115.5 kDa
• Gene Summary: This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]