BRMS1 (NM_015399) Human Tagged ORF Clone

CAT#: RG203428

BRMS1 (GFP-tagged) - Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BRMS1
• Synonyms: DKFZp564A063
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203428 representing NM_015399
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGTCCAGCCTCCAAGCAAAGACACAGAAGAGATGGAAGCAGAGGGTGATTCTGCTGCTGAGATGA
  ATGGGGAGGAGGAAGAGAGTGAGGAGGAGCGGAGCGGCAGCCAGACAGAGTCAGAAGAGGAGAGCTCCGA
  GATGGATGATGAGGACTATGAGCGACGCCGCAGCGAGTGTGTCAGTGAGATGCTGGACCTAGAGAAGCAG
  TTCTCGGAGCTAAAGGAGAAGTTGTTCAGGGAACGACTGAGTCAGCTGCGGTTGCGGCTGGAGGAAGTGG
  GGGCTGAGAGAGCCCCTGAATACACGGAGCCCCTTGGGGGGCTGCAGCGGAGCCTCAAGATTCGCATTCA
  GGTGGCAGGGATCTACAAGGGCTTCTGTCTGGATGTGATCAGGAATAAGTACGAATGTGAGCTGCAGGGA
  GCCAAACAGCACCTGGAGAGTGAGAAGCTGCTGCTCTATGACACGCTGCAGGGGGAGCTGCAGGAGCGGA
  TCCAGAGGCTGGAGGAGGACCGCCAGAGCCTGGATCTCAGCTCTGAATGGTGGGACGACAAACTGCACGC
  CAGAGGCAGCTCCAGGTCTTGGGACTCCCTGCCGCCCAGCAAGAGGAAGAAGGCACCTCTGGTTTCTGGC
  CCATACATCGTGTACATGCTTCAAGAGATCGACATCCTGGAGGACTGGACAGCCATCAAAAAGGCTAGGG
  CAGCTGTGTCCCCTCAGAAGAGAAAATCGGATGGACCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203428 representing NM_015399
  Red=Cloning site Green=Tags(s)
  
  MPVQPPSKDTEEMEAEGDSAAEMNGEEEESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQ
  FSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQRSLKIRIQVAGIYKGFCLDVIRNKYECELQG
  AKQHLESEKLLLYDTLQGELQERIQRLEEDRQSLDLSSEWWDDKLHARGSSRSWDSLPPSKRKKAPLVSG
  PYIVYMLQEIDILEDWTAIKKARAAVSPQKRKSDGP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015399
• ORF Size: 738 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015399.3, NP_056214.1
• RefSeq Size: 1455
• RefSeq ORF: 741
• Locus ID: 25855
• Protein Families: Druggable Genome
• Gene Summary: This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]