C5R1 (C5AR1) (NM_001736) Human Tagged ORF Clone

CAT#: RG203784

C5AR1 (GFP-tagged) - Human complement component 5a receptor 1 (C5AR1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C5AR1
• Synonyms: C5A; C5AR; C5R1; CD88
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203784 representing NM_001736
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCCTTCAATTATACCACCCCTGATTATGGGCACTATGATGACAAGGATACCCTGGACCTCAACA
  CCCCTGTGGATAAAACTTCTAACACGCTGCGTGTTCCAGACATCCTGGCCTTGGTCATCTTTGCAGTCGT
  CTTCCTGGTGGGAGTGCTGGGCAATGCCCTGGTGGTCTGGGTGACGGCATTCGAGGCCAAGCGGACCATC
  AATGCCATCTGGTTCCTCAACTTGGCGGTAGCCGACTTCCTCTCCTGCCTGGCGCTGCCCATCTTGTTCA
  CGTCCATTGTACAGCATCACCACTGGCCCTTTGGCGGGGCCGCCTGCAGCATCCTGCCCTCCCTCATCCT
  GCTCAACATGTACGCCAGCATCCTGCTCCTGGCCACCATCAGCGCCGACCGCTTTCTGCTGGTGTTTAAA
  CCCATCTGGTGCCAGAACTTCCGAGGGGCCGGCTTGGCCTGGATCGCCTGTGCCGTGGCTTGGGGTTTAG
  CCCTGCTGCTGACCATACCCTCCTTCCTGTACCGGGTGGTCCGGGAGGAGTACTTTCCACCAAAGGTGTT
  GTGTGGCGTGGACTACAGCCACGACAAACGGCGGGAGCGAGCCGTGGCCATCGTCCGGCTGGTCCTGGGC
  TTCCTGTGGCCTCTACTCACGCTCACGATTTGTTACACTTTCATCCTGCTCCGGACGTGGAGCCGCAGGG
  CCACGCGGTCCACCAAGACACTCAAGGTGGTGGTGGCAGTGGTGGCCAGTTTCTTTATCTTCTGGTTGCC
  CTACCAGGTGACGGGGATAATGATGTCCTTCCTGGAGCCATCGTCACCCACCTTCCTGCTGCTGAATAAG
  CTGGACTCCCTGTGTGTCTCCTTTGCCTACATCAACTGCTGCATCAACCCCATCATCTACGTGGTGGCCG
  GCCAGGGCTTCCAGGGCCGACTGCGGAAATCCCTCCCCAGCCTCCTCCGGAACGTGTTGACTGAAGAGTC
  CGTGGTTAGGGAGAGCAAGTCATTCACGCGCTCCACAGTGGACACTATGGCCCAGAAGACCCAGGCAGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203784 representing NM_001736
  Red=Cloning site Green=Tags(s)
  
  MNSFNYTTPDYGHYDDKDTLDLNTPVDKTSNTLRVPDILALVIFAVVFLVGVLGNALVVWVTAFEAKRTI
  NAIWFLNLAVADFLSCLALPILFTSIVQHHHWPFGGAACSILPSLILLNMYASILLLATISADRFLLVFK
  PIWCQNFRGAGLAWIACAVAWGLALLLTIPSFLYRVVREEYFPPKVLCGVDYSHDKRRERAVAIVRLVLG
  FLWPLLTLTICYTFILLRTWSRRATRSTKTLKVVVAVVASFFIFWLPYQVTGIMMSFLEPSSPTFLLLNK
  LDSLCVSFAYINCCINPIIYVVAGQGFQGRLRKSLPSLLRNVLTEESVVRESKSFTRSTVDTMAQKTQAV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001736
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001736.3, NP_001727.1
• RefSeq Size: 2342 bp
• RefSeq ORF: 1053 bp
• Locus ID: 728
• Cytogenetics: 19q13.32
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Neuroactive ligand-receptor interaction
• Gene Summary: ''