CTBP1 (NM_001012614) Human Tagged ORF Clone

CAT#: RG208594

CTBP1 (GFP-tagged) - Human C-terminal binding protein 1 (CTBP1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CTBP1
• Synonyms: BARS; HADDTS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208594 representing NM_001012614
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGCGTCCGACCTCCGATCATGAACGGGCCCCTGCACCCGCGGCCCCTGGTGGCATTGCTGGATG
  GCCGGGACTGCACAGTGGAGATGCCCATCCTGAAGGACGTGGCCACTGTGGCCTTCTGCGACGCGCAGTC
  CACGCAGGAGATCCATGAGAAGGTCCTGAACGAGGCTGTGGGGGCCCTGATGTACCACACCATCACTCTC
  ACCAGGGAGGACCTGGAGAAGTTCAAAGCCCTCCGCATCATCGTCCGGATTGGCAGTGGTTTTGACAACA
  TCGACATCAAGTCGGCCGGGGATTTAGGCATTGCCGTCTGCAACGTGCCCGCGGCGTCTGTGGAGGAGAC
  GGCCGACTCGACGCTGTGCCACATCCTGAACCTGTACCGGCGGGCCACCTGGCTGCACCAGGCGCTGCGG
  GAGGGCACACGAGTCCAGAGCGTCGAGCAGATCCGCGAGGTGGCGTCCGGCGCTGCCAGGATCCGCGGGG
  AGACCTTGGGCATCATCGGACTTGGTCGCGTGGGGCAGGCAGTGGCGCTGCGGGCCAAGGCCTTCGGCTT
  CAACGTGCTCTTCTACGACCCTTACTTGTCGGATGGCGTGGAGCGGGCGCTGGGGCTGCAGCGTGTCAGC
  ACCCTGCAGGACCTGCTCTTCCACAGCGACTGCGTGACCCTGCACTGCGGCCTCAACGAGCACAACCACC
  ACCTCATCAACGACTTCACCGTCAAGCAGATGAGACAAGGGGCCTTCCTGGTGAACACAGCCCGGGGTGG
  CCTGGTGGATGAGAAGGCGCTGGCCCAGGCCCTGAAGGAGGGCCGGATCCGCGGCGCGGCCCTGGATGTG
  CACGAGTCGGAACCCTTCAGCTTTAGCCAGGGCCCTCTGAAGGATGCACCCAACCTCATCTGCACCCCCC
  ATGCTGCATGGTACAGCGAGCAGGCATCCATCGAGATGCGAGAGGAGGCGGCACGGGAGATCCGCAGAGC
  CATCACAGGCCGGATCCCAGACAGCCTGAAGAACTGTGTCAACAAGGACCATCTGACAGCCGCCACCCAC
  TGGGCCAGCATGGACCCCGCCGTCGTGCACCCTGAGCTCAATGGGGCTGCCTATAGGTACCCTCCGGGCG
  TGGTGGGCGTGGCCCCCACTGGCATCCCAGCTGCTGTGGAAGGTATCGTCCCCAGCGCCATGTCCCTGTC
  CCACGGCCTGCCCCCTGTGGCCCACCCGCCCCACGCCCCTTCTCCTGGCCAAACCGTCAAGCCCGAGGCG
  GATAGAGACCACGCCAGTGACCAGTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208594 representing NM_001012614
  Red=Cloning site Green=Tags(s)
  
  MSGVRPPIMNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITL
  TREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRATWLHQALR
  EGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGVERALGLQRVS
  TLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDV
  HESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATH
  WASMDPAVVHPELNGAAYRYPPGVVGVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEA
  DRDHASDQL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012614
• ORF Size: 1287 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012614.1, NP_001012632.1
• RefSeq Size: 2483 bp
• RefSeq ORF: 1290 bp
• Locus ID: 1487
• Cytogenetics: 4p16.3
• Protein Pathways: Chronic myeloid leukemia, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
• Gene Summary: 'This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]'