DDX47 (NM_016355) Human Tagged ORF Clone

CAT#: RG209448

DDX47 (GFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 47 (DDX47), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DDX47
• Synonyms: E4-DBP; HQ0256; MSTP162; RRP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209448 representing NM_016355
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCACCCGAGGAACACGATTCTCCGACCGAAGCGTCCCAGCCGATTGTGGAAGAGGAGGAAACTA
  AAACATTTAAAGACCTGGGTGTGACAGATGTGTTGTGTGAAGCTTGTGACCAGTTGGGATGGACAAAACC
  CACCAAGATCCAGATTGAAGCTATTCCTTTGGCCTTACAAGGTCGTGATATCATTGGGCTTGCAGAAACT
  GGCTCTGGAAAGACAGGCGCCTTTGCTTTGCCCATTCTAAACGCACTGCTGGAGACCCCGCAGCGTTTGT
  TTGCCCTAGTTCTTACCCCGACTCGGGAGCTGGCCTTTCAGATCTCAGAGCAGTTTGAAGCCCTGGGGTC
  CTCTATTGGAGTGCAGAGTGCTGTGATTGTAGGTGGAATTGATTCAATGTCTCAATCTTTGGCCCTTGCA
  AAAAAACCACATATAATAATAGCAACTCCTGGTCGACTGATTGACCACTTGGAAAATACGAAAGGTTTCA
  ACTTGAGAGCTCTCAAATACTTGGTCATGGATGAAGCCGACCGAATACTGAATATGGATTTTGAGACAGA
  GGTTGACAAGATCCTCAAAGTGATTCCTCGAGATCGGAAAACATTCCTCTTCTCTGCCACCATGACCAAG
  AAGGTTCAAAAACTTCAGCGAGCAGCTCTGAAGAATCCTGTGAAATGTGCCGTTTCCTCTAAATACCAGA
  CAGTTGAAAAATTACAGCAATATTATATTTTTATTCCCTCTAAATTCAAGGATACCTACCTGGTTTATAT
  TCTAAATGAATTGGCTGGAAACTCCTTTATGATATTCTGCAGCACCTGTAATAATACCCAGAGAACAGCT
  TTGCTACTGCGAAATCTTGGCTTCACTGCCATCCCCCTCCATGGACAAATGAGTCAGAGTAAGCGCCTAG
  GATCCCTTAATAAGTTTAAGGCCAAGGCCCGTTCCATTCTTCTAGCAACTGACGTTGCCAGCCGAGGTTT
  GGACATACCTCATGTAGATGTGGTTGTCAACTTTGACATTCCTACCCATTCCAAGGATTACATCCATCGA
  GTAGGTCGAACAGCTAGAGCTGGGCGCTCCGGAAAGGCTATTACTTTTGTCACACAGTATGATGTGGAAC
  TCTTCCAGCGCATAGAACACTTAATTGGGAAGAAACTACCAGGTTTTCCAACACAGGATGATGAGGTTAT
  GATGCTGACAGAACGCGTCGCTGAAGCCCAAAGGTTTGCCCGAATGGAGTTAAGGGAGCATGGAGAAAAG
  AAGAAACGCTCGCGAGAGGATGCTGGAGATAATGATGACACAGAGGGTGCTATTGGTGTCAGGAACAAGG
  TGGCTGGAGGAAAAATGAAGAAGCGGAAAGGCCGT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209448 representing NM_016355
  Red=Cloning site Green=Tags(s)
  
  MAAPEEHDSPTEASQPIVEEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQGRDIIGLAET
  GSGKTGAFALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIGVQSAVIVGGIDSMSQSLALA
  KKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRDRKTFLFSATMTK
  KVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTA
  LLLRNLGFTAIPLHGQMSQSKRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHR
  VGRTARAGRSGKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEK
  KKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016355
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016355.3, NP_057439.2
• RefSeq Size: 1836
• RefSeq ORF: 1368
• Locus ID: 51202
• Domains: DEAD, helicase_C
• Gene Summary: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]