CCKAR (NM_000730) Human Tagged ORF Clone
CAT#: RG210435
- TrueORF®
CCKAR (GFP-tagged) - Human cholecystokinin A receptor (CCKAR)
"NM_000730" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | CCKAR |
Synonyms | CCK-A; CCK1-R; CCK1R; CCKRA |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG210435 representing NM_000730
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTGGTTGACAGCCTTCTTGTGAATGGAAGCAACATCACTCCTCCCTGTGAACTCGGGCTCGAAA ATGAGACGCTTTTCTGCTTGGATCAGCCCCGTCCTTCCAAAGAGTGGCAGCCAGCGGTGCAGATTCTCTT GTACTCCTTGATATTCCTGCTCAGCGTGCTGGGAAACACGCTGGTCATCACCGTGCTGATTCGGAACAAG CGGATGCGGACGGTCACCAACATCTTCCTCCTCTCCCTGGCTGTCAGCGACCTCATGCTCTGTCTCTTCT GCATGCCGTTCAACCTCATCCCCAATCTGCTCAAGGATTTCATCTTCGGGAGCGCCGTTTGCAAGACCAC CACCTACTTCATGGGCACCTCTGTGAGTGTATCTACCTTTAATCTGGTAGCCATATCTCTAGAGAGATAT GGTGCGATTTGCAAACCCTTACAGTCCCGGGTCTGGCAGACAAAATCCCATGCTTTGAAGGTGATTGCTG CTACCTGGTGCCTTTCCTTTACCATCATGACTCCGTACCCCATTTATAGCAACTTGGTGCCTTTTACCAA AAATAACAACCAGACCGCGAATATGTGCCGCTTTCTACTGCCAAATGATGTTATGCAGCAGTCCTGGCAC ACATTCCTGTTACTCATCCTCTTTCTTATTCCTGGAATTGTGATGATGGTGGCATATGGATTAATCTCTT TGGAACTCTACCAGGGAATAAAATTTGAGGCTAGCCAGAAGAAGTCTGCTAAAGAAAGGAAACCTAGCAC CACCAGCAGCGGCAAATATGAGGACAGCGATGGGTGTTACCTGCAAAAGACCAGGCCCCCGAGGAAGCTG GAGCTCCGGCAGCTGTCCACCGGCAGCAGCAGCAGGGCCAACCGCATCCGGAGTAACAGCTCCGCAGCCA ACCTGATGGCCAAGAAAAGGGTGATCCGCATGCTCATCGTCATCGTGGTCCTCTTCTTCCTGTGCTGGAT GCCCATCTTCAGCGCCAACGCCTGGCGGGCCTACGACACCGCCTCCGCAGAGCGCCGCCTCTCAGGAACC CCCATTTCCTTCATCCTCCTCCTGTCCTACACCTCCTCCTGCGTCAACCCCATCATCTACTGCTTCATGA ACAAACGCTTCCGCCTCGGCTTCATGGCCACCTTCCCCTGCTGCCCCAATCCTGGTCCCCCAGGGGCGAG GGGAGAGGTGGGGGAGGAGGAGGAAGGCGGGACCACAGGAGCCTCTCTGTCCAGGTTCTCGTACAGCCAT ATGAGTGCCTCGGTGCCACCCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210435 representing NM_000730
Red=Cloning site Green=Tags(s) MDVVDSLLVNGSNITPPCELGLENETLFCLDQPRPSKEWQPAVQILLYSLIFLLSVLGNTLVITVLIRNK RMRTVTNIFLLSLAVSDLMLCLFCMPFNLIPNLLKDFIFGSAVCKTTTYFMGTSVSVSTFNLVAISLERY GAICKPLQSRVWQTKSHALKVIAATWCLSFTIMTPYPIYSNLVPFTKNNNQTANMCRFLLPNDVMQQSWH TFLLLILFLIPGIVMMVAYGLISLELYQGIKFEASQKKSAKERKPSTTSSGKYEDSDGCYLQKTRPPRKL ELRQLSTGSSSRANRIRSNSSAANLMAKKRVIRMLIVIVVLFFLCWMPIFSANAWRAYDTASAERRLSGT PISFILLLSYTSSCVNPIIYCFMNKRFRLGFMATFPCCPNPGPPGARGEVGEEEEGGTTGASLSRFSYSH MSASVPPQ TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000730 |
ORF Size | 1284 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000730.2, NP_000721.1 |
RefSeq Size | 1682 bp |
RefSeq ORF | 1287 bp |
Locus ID | 886 |
Cytogenetics | 4p15.2 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Calcium signaling pathway, Neuroactive ligand-receptor interaction |
Gene Summary | 'This gene encodes a G-protein coupled receptor that binds non-sulfated members of the cholecystokinin (CCK) family of peptide hormones. This receptor is a major physiologic mediator of pancreatic enzyme secretion and smooth muscle contraction of the gallbladder and stomach. In the central and peripheral nervous system this receptor regulates satiety and the release of beta-endorphin and dopamine. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC300127 | CCKAR (untagged)-Human cholecystokinin A receptor (CCKAR) |
USD 760.00 |
|
RC210435 | CCKAR (Myc-DDK-tagged)-Human cholecystokinin A receptor (CCKAR) |
USD 420.00 |
|
RC210435L1 | Lenti ORF clone of Human cholecystokinin A receptor (CCKAR), Myc-DDK-tagged |
USD 768.00 |
|
RC210435L2 | Lenti ORF clone of Human cholecystokinin A receptor (CCKAR), mGFP tagged |
USD 620.00 |
|
RC210435L3 | Lenti ORF clone of Human cholecystokinin A receptor (CCKAR), Myc-DDK-tagged |
USD 768.00 |
|
RC210435L4 | Lenti ORF clone of Human cholecystokinin A receptor (CCKAR), mGFP tagged |
USD 620.00 |
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