Bombesin Receptor 3 (BRS3) (NM_001727) Human Tagged ORF Clone

CAT#: RG213923

BRS3 (GFP-tagged) - Human bombesin-like receptor 3 (BRS3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BRS3
• Synonyms: BB3; BB3R; BBR3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213923 representing NM_001727
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAAAGGCAGCCTCACTCACCTAATCAGACTTTAATTTCAATCACAAATGACACAGAATCATCAA
  GCTCTGTGGTTTCTAACGATAACACAAATAAAGGATGGAGCGGGGACAACTCTCCAGGAATAGAAGCATT
  GTGTGCCATCTATATTACTTATGCTGTGATCATTTCAGTGGGCATCCTTGGAAATGCTATTCTCATCAAA
  GTCTTTTTCAAGACCAAATCCATGCAAACAGTTCCAAATATTTTCATCACCAGCCTGGCTTTTGGAGATC
  TTTTACTTCTGCTAACTTGTGTGCCAGTGGATGCAACTCACTACCTTGCAGAAGGATGGCTGTTCGGAAG
  AATTGGTTGTAAGGTGCTCTCTTTCATCCGGCTCACTTCTGTTGGTGTGTCAGTGTTCACATTAACAATT
  CTCAGCGCTGACAGATACAAGGCAGTTGTGAAGCCACTTGAGCGACAGCCCTCCAATGCCATCCTGAAGA
  CTTGTGTAAAAGCTGGCTGCGTCTGGATCGTGTCTATGATATTTGCTCTACCTGAGGCTATATTTTCAAA
  TGTATACACTTTTCGAGATCCCAATAAAAATATGACATTTGAATCATGTACCTCTTATCCTGTCTCTAAG
  AAGCTCTTGCAAGAAATACATTCTCTGCTGTGCTTCTTAGTGTTCTACATTATTCCACTCTCTATTATCT
  CTGTCTACTATTCCTTGATTGCTAGGACCCTTTACAAAAGCACCCTGAACATACCTACTGAGGAACAAAG
  CCATGCCCGTAAGCAGATTGAATCCCGAAAGAGAATTGCCAGAACGGTATTGGTGTTGGTGGCTCTGTTT
  GCCCTCTGCTGGTTGCCAAATCACCTCCTGTACCTCTACCATTCATTCACTTCTCAAACCTATGTAGACC
  CCTCTGCCATGCATTTCATTTTCACCATTTTCTCTCGGGTTTTGGCTTTCAGCAATTCTTGCGTAAACCC
  CTTTGCTCTCTACTGGCTGAGCAAAAGCTTCCAGAAGCATTTTAAAGCTCAGTTGTTCTGTTGCAAGGCG
  GAGCGGCCTGAGCCTCCTGTTGCTGACACCTCTCTTACCACCCTGGCTGTGATGGGAACGGTCCCGGGCA
  CTGGGAGCATACAGATGTCTGAAATTAGTGTGACCTCGTTCACTGGGTGTAGTGTGAAGCAGGCAGAGGA
  CAGATTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213923 representing NM_001727
  Red=Cloning site Green=Tags(s)
  
  MAQRQPHSPNQTLISITNDTESSSSVVSNDNTNKGWSGDNSPGIEALCAIYITYAVIISVGILGNAILIK
  VFFKTKSMQTVPNIFITSLAFGDLLLLLTCVPVDATHYLAEGWLFGRIGCKVLSFIRLTSVGVSVFTLTI
  LSADRYKAVVKPLERQPSNAILKTCVKAGCVWIVSMIFALPEAIFSNVYTFRDPNKNMTFESCTSYPVSK
  KLLQEIHSLLCFLVFYIIPLSIISVYYSLIARTLYKSTLNIPTEEQSHARKQIESRKRIARTVLVLVALF
  ALCWLPNHLLYLYHSFTSQTYVDPSAMHFIFTIFSRVLAFSNSCVNPFALYWLSKSFQKHFKAQLFCCKA
  ERPEPPVADTSLTTLAVMGTVPGTGSIQMSEISVTSFTGCSVKQAEDRF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001727
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001727.1, NP_001718.1
• RefSeq Size: 1413 bp
• RefSeq ORF: 1200 bp
• Locus ID: 680
• Cytogenetics: Xq26.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'The protein encoded by this gene is a G protein-coupled membrane receptor that binds bombesin-like peptides. This binding results in activation of a phosphatidylinositol-calcium second messenger system, with physiological effects including regulation of metabolic rate, glucose metabolism, and hypertension. [provided by RefSeq, Sep 2011]'