CD35 (CR1) (NM_000573) Human Tagged ORF Clone

CAT#: RG216533

CR1 (GFP-tagged) - Human complement component (3b/4b) receptor 1 (Knops blood group) (CR1), transcript variant F

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CR1
• Synonyms: C3BR; C4BR; CD35; KN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216533 representing NM_000573
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCCTCTTCTCCAAGAAGCCCGGAGCCTGTCGGGCCGCCGGCGCCCGGTCTCCCCTTCTGCTGCG
  GAGGATCCCTGCTGGCGGTTGTGGTGCTGCTTGCGCTGCCGGTGGCCTGGGGTCAATGCAATGCCCCAGA
  ATGGCTTCCATTTGCCAGGCCTACCAACCTAACTGATGAATTTGAGTTTCCCATTGGGACATATCTGAAC
  TATGAATGCCGCCCTGGTTATTCCGGAAGACCGTTTTCTATCATCTGCCTAAAAAACTCAGTCTGGACTG
  GTGCTAAGGACAGGTGCAGACGTAAATCATGTCGTAATCCTCCAGATCCTGTGAATGGCATGGTGCATGT
  GATCAAAGGCATCCAGTTCGGATCCCAAATTAAATATTCTTGTACTAAAGGATACCGACTCATTGGTTCC
  TCGTCTGCCACATGCATCATCTCAGGTGATACTGTCATTTGGGATAATGAAACACCTATTTGTGACAGAA
  TTCCTTGTGGGCTACCCCCCACCATCACCAATGGAGATTTCATTAGCACCAACAGAGAGAATTTTCACTA
  TGGATCAGTGGTGACCTACCGCTGCAATCCTGGAAGCGGAGGGAGAAAGGTGTTTGAGCTTGTGGGTGAG
  CCCTCCATATACTGCACCAGCAATGACGATCAAGTGGGCATCTGGAGCGGCCCCGCCCCTCAGTGCATTA
  TACCTAACAAATGCACGCCTCCAAATGTGGAAAATGGAATATTGGTATCTGACAACAGAAGCTTATTTTC
  CTTAAATGAAGTTGTGGAGTTTAGGTGTCAGCCTGGCTTTGTCATGAAAGGACCCCGCCGTGTGAAGTGC
  CAGGCCCTGAACAAATGGGAGCCGGAGCTACCAAGCTGCTCCAGGGTATGTCAGCCACCTCCAGATGTCC
  TGCATGCTGAGCGTACCCAAAGGGACAAGGACAACTTTTCACCTGGGCAGGAAGTGTTCTACAGCTGTGA
  GCCCGGCTACGACCTCAGAGGGGCTGCGTCTATGCGCTGCACACCCCAGGGAGACTGGAGCCCTGCAGCC
  CCCACATGTGAAGTGAAATCCTGTGATGACTTCATGGGCCAACTTCTTAATGGCCGTGTGCTATTTCCAG
  TAAATCTCCAGCTTGGAGCAAAAGTGGATTTTGTTTGTGATGAAGGATTTCAATTAAAAGGCAGCTCTGC
  TAGTTACTGTGTCTTGGCTGGAATGGAAAGCCTTTGGAATAGCAGTGTTCCAGTGTGTGAACAAATCTTT
  TGTCCAAGTCCTCCAGTTATTCCTAATGGGAGACACACAGGAAAACCTCTGGAAGTCTTTCCCTTTGGGA
  AAACAGTAAATTACACATGCGACCCCCACCCAGACAGAGGGACGAGCTTCGACCTCATTGGAGAGAGCAC
  CATCCGCTGCACAAGTGACCCTCAAGGGAATGGGGTTTGGAGCAGCCCTGCCCCTCGCTGTGGAATTCTG
  GGTCACTGTCAAGCCCCAGATCATTTTCTGTTTGCCAAGTTGAAAACCCAAACCAATGCATCTGACTTTC
  CCATTGGGACATCTTTAAAGTACGAATGCCGTCCTGAGTACTACGGGAGGCCATTCTCTATCACATGTCT
  AGATAACCTGGTCTGGTCAAGTCCCAAAGATGTCTGTAAACGTAAATCATGTAAAACTCCTCCAGATCCA
  GTGAATGGCATGGTGCATGTGATCACAGACATCCAGGTTGGATCCAGAATCAACTATTCTTGTACTACAG
  GGCACCGACTCATTGGTCACTCATCTGCTGAATGTATCCTCTCGGGCAATGCTGCCCATTGGAGCACGAA
  GCCGCCAATTTGTCAACGAATTCCTTGTGGGCTACCCCCCACCATCGCCAATGGAGATTTCATTAGCACC
  AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCCTGGAAGCGGAGGGAGAAAGG
  TGTTTGAGCTTGTGGGTGAGCCCTCCATATACTGCACCAGCAATGACGATCAAGTGGGCATCTGGAGCGG
  CCCGGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAAATGGAATATTGGTATCT
  GACAACAGAAGCTTATTTTCCTTAAATGAAGTTGTGGAGTTTAGGTGTCAGCCTGGCTTTGTCATGAAAG
  GACCCCGCCGTGTGAAGTGCCAGGCCCTGAACAAATGGGAGCCGGAGCTACCAAGCTGCTCCAGGGTATG
  TCAGCCACCTCCAGATGTCCTGCATGCTGAGCGTACCCAAAGGGACAAGGACAACTTTTCACCCGGGCAG
  GAAGTGTTCTACAGCTGTGAGCCCGGCTATGACCTCAGAGGGGCTGCGTCTATGCGCTGCACACCCCAGG
  GAGACTGGAGCCCTGCAGCCCCCACATGTGAAGTGAAATCCTGTGATGACTTCATGGGCCAACTTCTTAA
  TGGCCGTGTGCTATTTCCAGTAAATCTCCAGCTTGGAGCAAAAGTGGATTTTGTTTGTGATGAAGGATTT
  CAATTAAAAGGCAGCTCTGCTAGTTATTGTGTCTTGGCTGGAATGGAAAGCCTTTGGAATAGCAGTGTTC
  CAGTGTGTGAACAAATCTTTTGTCCAAGTCCTCCAGTTATTCCTAATGGGAGACACACAGGAAAACCTCT
  GGAAGTCTTTCCCTTTGGAAAAGCAGTAAATTACACATGCGACCCCCACCCAGACAGAGGGACGAGCTTC
  GACCTCATTGGAGAGAGCACCATCCGCTGCACAAGTGACCCTCAAGGGAATGGGGTTTGGAGCAGCCCTG
  CCCCTCGCTGTGGAATTCTGGGTCACTGTCAAGCCCCAGATCATTTTCTGTTTGCCAAGTTGAAAACCCA
  AACCAATGCATCTGACTTTCCCATTGGGACATCTTTAAAGTACGAATGCCGTCCTGAGTACTACGGGAGG
  CCATTCTCTATCACATGTCTAGATAACCTGGTCTGGTCAAGTCCCAAAGATGTCTGTAAACGTAAATCAT
  GTAAAACTCCTCCAGATCCAGTGAATGGCATGGTGCATGTGATCACAGACATCCAGGTTGGATCCAGAAT
  CAACTATTCTTGTACTACAGGGCACCGACTCATTGGTCACTCATCTGCTGAATGTATCCTCTCAGGCAAT
  ACTGCCCATTGGAGCACGAAGCCGCCAATTTGTCAACGAATTCCTTGTGGGCTACCCCCAACCATCGCCA
  ATGGAGATTTCATTAGCACCAACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCT
  TGGAAGCAGAGGGAGAAAGGTGTTTGAGCTTGTGGGTGAGCCCTCCATATACTGCACCAGCAATGACGAT
  CAAGTGGGCATCTGGAGCGGCCCCGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGG
  AAAATGGAATATTGGTATCTGACAACAGAAGCTTATTTTCCTTAAATGAAGTTGTGGAGTTTAGGTGTCA
  GCCTGGCTTTGTCATGAAAGGACCCCGCCGTGTGAAGTGCCAGGCCCTGAACAAATGGGAGCCAGAGTTA
  CCAAGCTGCTCCAGGGTGTGTCAGCCGCCTCCAGAAATCCTGCATGGTGAGCATACCCCAAGCCATCAGG
  ACAACTTTTCACCTGGGCAGGAAGTGTTCTACAGCTGTGAGCCTGGCTATGACCTCAGAGGGGCTGCGTC
  TCTGCACTGCACACCCCAGGGAGACTGGAGCCCTGAAGCCCCGAGATGTGCAGTGAAATCCTGTGATGAC
  TTCTTGGGTCAACTCCCTCATGGCCGTGTGCTATTTCCACTTAATCTCCAGCTTGGGGCAAAGGTGTCCT
  TTGTCTGTGATGAAGGGTTTCGCTTAAAGGGCAGTTCCGTTAGTCATTGTGTCTTGGTTGGAATGAGAAG
  CCTTTGGAATAACAGTGTTCCTGTGTGTGAACATATCTTTTGTCCAAATCCTCCAGCTATCCTTAATGGG
  AGACACACAGGAACTCCCTCTGGAGATATTCCCTATGGAAAAGAAATATCTTACACATGTGACCCCCACC
  CAGACAGAGGGATGACCTTCAACCTCATTGGGGAGAGCACCATCCGCTGCACAAGTGACCCTCATGGGAA
  TGGGGTTTGGAGCAGCCCTGCCCCTCGCTGTGAACTTTCTGTTCGTGCTGGTCACTGTAAAACCCCAGAG
  CAGTTTCCATTTGCCAGTCCTACGATCCCAATTAATGACTTTGAGTTTCCAGTCGGGACATCTTTGAATT
  ATGAATGCCGTCCTGGGTATTTTGGGAAAATGTTCTCTATCTCCTGCCTAGAAAACTTGGTCTGGTCAAG
  TGTTGAAGACAACTGTAGACGAAAATCATGTGGACCTCCACCAGAACCCTTCAATGGAATGGTGCATATA
  AACACAGATACACAGTTTGGATCAACAGTTAATTATTCTTGTAATGAAGGGTTTCGACTCATTGGTTCCC
  CATCTACTACTTGTCTCGTCTCAGGCAATAATGTCACATGGGATAAGAAGGCACCTATTTGTGAGATCAT
  ATCTTGTGAGCCACCTCCAACCATATCCAATGGAGACTTCTACAGCAACAATAGAACATCTTTTCACAAT
  GGAACGGTGGTAACTTACCAGTGCCACACTGGACCAGATGGAGAACAGCTGTTTGAGCTTGTGGGAGAAC
  GGTCAATATATTGCACCAGCAAAGATGATCAAGTTGGTGTTTGGAGCAGCCCTCCCCCTCGGTGTATTTC
  TACTAATAAATGCACAGCTCCAGAAGTTGAAAATGCAATTAGAGTACCAGGAAACAGGAGTTTCTTTACC
  CTCACTGAGATCATCAGATTTAGATGTCAGCCCGGGTTTGTCATGGTAGGGTCCCACACTGTGCAGTGCC
  AGACCAATGGCAGATGGGGGCCCAAGCTGCCACACTGCTCCAGGGTGTGTCAGCCGCCTCCAGAAATCCT
  GCATGGTGAGCATACCCTAAGCCATCAGGACAACTTTTCACCTGGGCAGGAAGTGTTCTACAGCTGTGAG
  CCCAGCTATGACCTCAGAGGGGCTGCGTCTCTGCACTGCACGCCCCAGGGAGACTGGAGCCCTGAAGCCC
  CTAGATGTACAGTGAAATCCTGTGATGACTTCCTGGGCCAACTCCCTCATGGCCGTGTGCTACTTCCACT
  TAATCTCCAGCTTGGGGCAAAGGTGTCCTTTGTTTGCGATGAAGGGTTCCGATTAAAAGGCAGGTCTGCT
  AGTCATTGTGTCTTGGCTGGAATGAAAGCCCTTTGGAATAGCAGTGTTCCAGTGTGTGAACAAATCTTTT
  GTCCAAATCCTCCAGCTATCCTTAATGGGAGACACACAGGAACTCCCTTTGGAGATATTCCCTATGGAAA
  AGAAATATCTTACGCATGCGACACCCACCCAGACAGAGGGATGACCTTCAACCTCATTGGGGAGAGCTCC
  ATCCGCTGCACAAGTGACCCTCAAGGGAATGGGGTTTGGAGCAGCCCTGCCCCTCGCTGTGAACTTTCTG
  TTCCTGCTGCCTGCCCACATCCACCCAAGATCCAAAACGGGCATTACATTGGAGGACACGTATCTCTATA
  TCTTCCTGGGATGACAATCAGCTACATTTGTGACCCCGGCTACCTGTTAGTGGGAAAGGGCTTCATTTTC
  TGTACAGACCAGGGAATCTGGAGCCAATTGGATCATTATTGCAAAGAAGTAAATTGTAGCTTCCCACTGT
  TTATGAATGGAATCTCGAAGGAGTTAGAAATGAAAAAAGTATATCACTATGGAGATTATGTGACTTTGAA
  GTGTGAAGATGGGTATACTCTGGAAGGCAGTCCCTGGAGCCAGTGCCAGGCGGATGACAGATGGGACCCT
  CCTCTGGCCAAATGTACCTCTCGTACACATGATGCTCTCATAGTTGGCACTTTATCTGGTACGATCTTCT
  TTATTTTACTCATCATTTTCCTCTCTTGGATAATTCTAAAGCACAGAAAAGGCAATAATGCACATGAAAA
  CCCTAAAGAAGTGGCTATCCATTTACATTCTCAAGGAGGCAGCAGCGTTCATCCCCGAACTCTGCAAACA
  AATGAAGAAAATAGCAGGGTCCTTCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216533 representing NM_000573
  Red=Cloning site Green=Tags(s)
  
  MGASSPRSPEPVGPPAPGLPFCCGGSLLAVVVLLALPVAWGQCNAPEWLPFARPTNLTDEFEFPIGTYLN
  YECRPGYSGRPFSIICLKNSVWTGAKDRCRRKSCRNPPDPVNGMVHVIKGIQFGSQIKYSCTKGYRLIGS
  SSATCIISGDTVIWDNETPICDRIPCGLPPTITNGDFISTNRENFHYGSVVTYRCNPGSGGRKVFELVGE
  PSIYCTSNDDQVGIWSGPAPQCIIPNKCTPPNVENGILVSDNRSLFSLNEVVEFRCQPGFVMKGPRRVKC
  QALNKWEPELPSCSRVCQPPPDVLHAERTQRDKDNFSPGQEVFYSCEPGYDLRGAASMRCTPQGDWSPAA
  PTCEVKSCDDFMGQLLNGRVLFPVNLQLGAKVDFVCDEGFQLKGSSASYCVLAGMESLWNSSVPVCEQIF
  CPSPPVIPNGRHTGKPLEVFPFGKTVNYTCDPHPDRGTSFDLIGESTIRCTSDPQGNGVWSSPAPRCGIL
  GHCQAPDHFLFAKLKTQTNASDFPIGTSLKYECRPEYYGRPFSITCLDNLVWSSPKDVCKRKSCKTPPDP
  VNGMVHVITDIQVGSRINYSCTTGHRLIGHSSAECILSGNAAHWSTKPPICQRIPCGLPPTIANGDFIST
  NRENFHYGSVVTYRCNPGSGGRKVFELVGEPSIYCTSNDDQVGIWSGPAPQCIIPNKCTPPNVENGILVS
  DNRSLFSLNEVVEFRCQPGFVMKGPRRVKCQALNKWEPELPSCSRVCQPPPDVLHAERTQRDKDNFSPGQ
  EVFYSCEPGYDLRGAASMRCTPQGDWSPAAPTCEVKSCDDFMGQLLNGRVLFPVNLQLGAKVDFVCDEGF
  QLKGSSASYCVLAGMESLWNSSVPVCEQIFCPSPPVIPNGRHTGKPLEVFPFGKAVNYTCDPHPDRGTSF
  DLIGESTIRCTSDPQGNGVWSSPAPRCGILGHCQAPDHFLFAKLKTQTNASDFPIGTSLKYECRPEYYGR
  PFSITCLDNLVWSSPKDVCKRKSCKTPPDPVNGMVHVITDIQVGSRINYSCTTGHRLIGHSSAECILSGN
  TAHWSTKPPICQRIPCGLPPTIANGDFISTNRENFHYGSVVTYRCNLGSRGRKVFELVGEPSIYCTSNDD
  QVGIWSGPAPQCIIPNKCTPPNVENGILVSDNRSLFSLNEVVEFRCQPGFVMKGPRRVKCQALNKWEPEL
  PSCSRVCQPPPEILHGEHTPSHQDNFSPGQEVFYSCEPGYDLRGAASLHCTPQGDWSPEAPRCAVKSCDD
  FLGQLPHGRVLFPLNLQLGAKVSFVCDEGFRLKGSSVSHCVLVGMRSLWNNSVPVCEHIFCPNPPAILNG
  RHTGTPSGDIPYGKEISYTCDPHPDRGMTFNLIGESTIRCTSDPHGNGVWSSPAPRCELSVRAGHCKTPE
  QFPFASPTIPINDFEFPVGTSLNYECRPGYFGKMFSISCLENLVWSSVEDNCRRKSCGPPPEPFNGMVHI
  NTDTQFGSTVNYSCNEGFRLIGSPSTTCLVSGNNVTWDKKAPICEIISCEPPPTISNGDFYSNNRTSFHN
  GTVVTYQCHTGPDGEQLFELVGERSIYCTSKDDQVGVWSSPPPRCISTNKCTAPEVENAIRVPGNRSFFT
  LTEIIRFRCQPGFVMVGSHTVQCQTNGRWGPKLPHCSRVCQPPPEILHGEHTLSHQDNFSPGQEVFYSCE
  PSYDLRGAASLHCTPQGDWSPEAPRCTVKSCDDFLGQLPHGRVLLPLNLQLGAKVSFVCDEGFRLKGRSA
  SHCVLAGMKALWNSSVPVCEQIFCPNPPAILNGRHTGTPFGDIPYGKEISYACDTHPDRGMTFNLIGESS
  IRCTSDPQGNGVWSSPAPRCELSVPAACPHPPKIQNGHYIGGHVSLYLPGMTISYICDPGYLLVGKGFIF
  CTDQGIWSQLDHYCKEVNCSFPLFMNGISKELEMKKVYHYGDYVTLKCEDGYTLEGSPWSQCQADDRWDP
  PLAKCTSRTHDALIVGTLSGTIFFILLIIFLSWIILKHRKGNNAHENPKEVAIHLHSQGGSSVHPRTLQT
  NEENSRVLP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000573
• ORF Size: 6117 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000573.3, NP_000564.2
• RefSeq Size: 8629 bp
• RefSeq ORF: 6120 bp
• Locus ID: 1378
• Cytogenetics: 1q32.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Hematopoietic cell lineage
• Gene Summary: 'This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The genome is polymorphic at this locus with allele-specific splice variants encoding different isoforms, based on the presence/absence of long homologous repeats (LHRs). The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in this gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus, sarcoidosis and Alzheimer's disease. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. [provided by RefSeq, May 2020]'