DACH2 (NM_053281) Human Tagged ORF Clone

CAT#: RG219595

DACH2 (GFP-tagged) - Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DACH2
• Synonyms: FLJ31391; MGC138545
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219595 representing NM_053281
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTCTCCGCATCTCCAGTGATCTCTGCAACTTCCAGCGGCGCCGGCGTCCCGGGGGGCTTATTCC
  GGGCCGAACCCCTGTACTCGACTCCCAGAGAGCCCCCTCGTCTTACTCCTAATATGATCAATAGTTTCGT
  GGTTAATAACCACAGCAACAGTGCCGGAGGCGGCGGCAGGGGCAACACCAACACCAACGAGTGCCGCATG
  GTCGACATGCACGGGATGAAGGTGGCTTCGTTCCTGATGGACGGCCAGGAACTGATCTGCCTGCCGCAAG
  TCTTTGATCTTTTTCTCAAGCACCTGGTGGGAGGCTTGCACACTGTGTACACCAAGCTGAAGAGACTGGA
  TATATCCCCCGTGGTGTGTACTGTTGAGCAGGTCCGGATCCTCCGCGGGCTGGGGGCCATCCAGCCCGGG
  GTAAACCGCTGCAAACTCATCACCAGGAAAGACTTCGAAACTTTGTTCACCGATTGCACCAATGCCAGAA
  GGAAGAGGCAAATGACAAGAAAACAAGCTGTTAACAGTTCAAGACCCGGCAGGCCCCCTAAGCGTTCTTT
  GGGAGTGTTGCAGGAAAATGCCCGCCTTCTGACCCATGCAGTCCCAGGCCTCTTATCGCCAGGACTTATC
  ACTCCGACAGGTATAACAGCTGCAGCGATGGCTGAGGCGATGAAACTTCAGAAGATGAAGCTTATGGCTA
  TGAACACTCTTCAGGGAAATGGAAGCCAAAATGGGACCGAATCAGAGCCTGATGATCTTAATTCTAACAC
  AGGTGGAAGTGAATCCTCCTGGGATAAAGATAAGATGCAGTCTCCATTTGCTGCACCTGGACCCCAACAT
  GGAATTGCTCATGCAGCCCTAGCTGGCCAGCCAGGCATTGGGGGTGCTCCAACCCTCAATCCACTGCAGC
  AGAACCACCTGCTAACCAATAGACTGGATCTGCCATTTATGATGATGCCTCATCCCCTACTTCCAGTCAG
  CTTACCTCCTGCATCAGTTGCCATGGCAATGAATCAGATGAACCATCTCAATACTATTGCCAACATGGCT
  GCTGCAGCACAGATTCACAGTCCACTCTCCAGAGCTGGTACCTCTGTTATAAAGGAGCGGATCCCAGAGA
  GTCCTTCTCCTGCTCCTTCTCTAGAAGAGAATCATCGTCCTGGGAGCCAGACCTCTTCCCACACCAGCAG
  CAGTGTGTCCAGCTCTCCCTCTCAGATGGATCATCATTTGGAAAGAATGGAAGAGGTACCAGTTCAAATT
  CCAATAATGAAGTCACCCTTGGACAAGATACAGCTGACTCCTGGGCAGGCATTGCCCGCTGGATTCCCTG
  GACCATTCATTTTTGCTGATAGTCTGTCCTCCGTGGAGACTCTGTTGACCAACATTCAGGGTCTGCTGAA
  AGTTGCTTTGGATAATGCTCGCATCCAGGAGAAGCAGATTCAACAAGAAAAGAAGGAGCTGCGACTGGAG
  CTCTATAGAGAGAGAGAAATTAGAGAAAACCTTGAAAGACAACTTGCAGTTGAGCTTCAAAGCAGAACTA
  CTATGCAAAAGCGCCTGAAGAAGGAGAAAAAAACCAAGAGAAAATTGCAGGAAGCCTTGGAATTTGAATC
  AAAGCGCCGGGAGCAAGTGGAGCAGGCACTTAAGCAAGCCACCACTAGTGACAGTGGCCTGAGGATGTTA
  AAAGATACTGGAATTCCAGATATTGAAATAGAAAACAATGGGACTCCTCATGATAGTGCTGCTATGCAAG
  GAGGTAACTATTACTGTTTAGAAATGGCACAACAGTTGTATTCAGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219595 representing NM_053281
  Red=Cloning site Green=Tags(s)
  
  MAVSASPVISATSSGAGVPGGLFRAEPLYSTPREPPRLTPNMINSFVVNNHSNSAGGGGRGNTNTNECRM
  VDMHGMKVASFLMDGQELICLPQVFDLFLKHLVGGLHTVYTKLKRLDISPVVCTVEQVRILRGLGAIQPG
  VNRCKLITRKDFETLFTDCTNARRKRQMTRKQAVNSSRPGRPPKRSLGVLQENARLLTHAVPGLLSPGLI
  TPTGITAAAMAEAMKLQKMKLMAMNTLQGNGSQNGTESEPDDLNSNTGGSESSWDKDKMQSPFAAPGPQH
  GIAHAALAGQPGIGGAPTLNPLQQNHLLTNRLDLPFMMMPHPLLPVSLPPASVAMAMNQMNHLNTIANMA
  AAAQIHSPLSRAGTSVIKERIPESPSPAPSLEENHRPGSQTSSHTSSSVSSSPSQMDHHLERMEEVPVQI
  PIMKSPLDKIQLTPGQALPAGFPGPFIFADSLSSVETLLTNIQGLLKVALDNARIQEKQIQQEKKELRLE
  LYREREIRENLERQLAVELQSRTTMQKRLKKEKKTKRKLQEALEFESKRREQVEQALKQATTSDSGLRML
  KDTGIPDIEIENNGTPHDSAAMQGGNYYCLEMAQQLYSA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053281
• ORF Size: 1797 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053281.2, NP_444511.1
• RefSeq Size: 2232
• RefSeq ORF: 1800
• Locus ID: 117154
• Domains: Ski_Sno
• Protein Families: Transcription Factors
• Gene Summary: This gene is one of two genes which encode a protein similar to the Drosophila protein dachshund, a transcription factor involved in cell fate determination in the eye, limb and genital disc of the fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions. This gene is located on the X chromosome and is subject to inactivation by DNA methylation. The encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role in premature ovarian failure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]