Contactin 1 (CNTN1) (NM_175038) Human Tagged ORF Clone

CAT#: RG220493

CNTN1 (GFP-tagged) - Human contactin 1 (CNTN1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CNTN1
• Synonyms: F3; GP135; MYPCN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220493 representing NM_175038
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATGTGGTTGCTGGTCAGTCATCTTGTGATAATATCTATTACTACCTGTTTAGCAGTTTCTGAGG
  AAGACAAAGGATTTGGACCAATTTTTGAAGAGCAGCCAATCAATACCATTTATCCAGAGGAATCACTGGA
  AGGAAAAGTCTCACTCAACTGTAGGGCACGAGCCAGCCCTTTCCCGGTTTACAAATGGAGAATGAATAAT
  GGGGACGTTGATCTCACAAGTGATCGATACAGTATGGTAGGAGGAAACCTTGTTATCAACAACCCTGACA
  AACAGAAAGATGCTGGAATATACTACTGTTTAGCATCTAATAACTACGGGATGGTCAGAAGCACTGAAGC
  AACCCTGAGCTTTGGATATCTTGATCCTTTCCCACCTGAGGAACGTCCTGAGGTCAGAGTAAAAGAAGGG
  AAAGGAATGGTGCTTCTCTGTGACCCCCCATACCATTTTCCAGATGATCTTAGCTATCGCTGGCTTCTAA
  ATGAATTTCCTGTATTTATCACAATGGATAAACGGCGATTTGTGTCTCAGACAAATGGCAATCTCTACAT
  TGCAAATGTTGAGGCTTCCGACAAAGGCAATTATTCCTGCTTTGTTTCCAGTCCTTCTATTACAAAGAGC
  GTGTTCAGCAAATTCATCCCACTCATTCCAATACCTGAACGAACAACAAAACCATATCCTGCTGATATTG
  TAGTTCAGTTCAAGGATGTATATGCATTGATGGGCCAAAATGTGACCTTAGAATGTTTTGCACTTGGAAA
  TCCTGTTCCGGATATCCGATGGCGGAAGGTTCTAGAACCAATGCCAAGCACTGCTGAGATTAGCACCTCT
  GGGGCTGTTCTTAAGATCTTCAATATTCAGCTAGAAGATGAAGGCATCTATGAATGTGAGGCTGAGAACA
  TTAGAGGAAAGGATAAACATCAAGCAAGAATTTATGTTCAAGCATTCCCTGAGTGGGTAGAACACATCAA
  TGACACAGAGGTGGACATAGGCAGTGATCTCTACTGGCCTTGTGTGGCCACAGGAAAGCCCATCCCTACA
  ATCCGATGGTTGAAAAATGGATATGCGTATCATAAAGGGGAATTAAGACTGTATGATGTGACTTTTGAAA
  ATGCCGGAATGTATCAGTGCATAGCTGAAAACACATATGGAGCCATTTATGCAAATGCTGAGTTGAAGAT
  CTTGGCGTTGGCTCCAACTTTTGAAATGAATCCTATGAAGAAAAAGATCCTGGCTGCTAAAGGTGGAAGG
  GTGATAATTGAATGCAAACCTAAAGCTGCACCGAAACCAAAGTTTTCATGGAGTAAAGGGACAGAGTGGC
  TTGTCAATAGCAGCAGAATACTCATTTGGGAAGATGGTAGCTTGGAAATCAACAACATTACAAGGAATGA
  TGGAGGTATCTATACATGCTTTGCAGAAAATAACAGAGGGAAAGCTAATAGCACTGGAACCCTTGTTATC
  ACAGATCCTACGCGAATTATATTGGCCCCAATTAATGCCGATATCACAGTTGGAGAAAACGCCACCATGC
  AGTGTGCTGCGTCCTTTGATCCTGCCTTGGATCTCACATTTGTTTGGTCCTTCAATGGCTATGTGATCGA
  TTTTAACAAAGAGAATATTCACTACCAGAGGAATTTTATGCTGGATTCCAATGGGGAATTACTAATCCGA
  AATGCGCAGCTGAAACATGCTGGAAGATACACATGCACTGCCCAGACAATTGTGGACAATTCTTCAGCTT
  CAGCTGACCTTGTAGTGAGAGGCCCTCCAGGCCCTCCAGGTGGTCTGAGAATAGAAGACATTAGAGCCAC
  TTCTGTGGCACTTACTTGGAGCCGTGGTTCAGACAATCATAGTCCTATTTCTAAATACACTATCCAGACC
  AAGACTATTCTTTCAGATGACTGGAAAGATGCAAAGACAGATCCCCCAATTATTGAAGGAAATATGGAGG
  CAGCAAGAGCAGTGGACTTAATCCCATGGATGGAGTATGAATTCCGCGTGGTAGCAACCAATACACTGGG
  TAGAGGAGAGCCCAGTATACCATCTAACAGAATTAAAACAGACGGTGCTGCACCAAATGTGGCTCCTTCA
  GATGTAGGAGGTGGAGGTGGAAGAAACAGAGAGCTGACCATAACATGGGCGCCTTTGTCAAGAGAATACC
  ACTATGGCAACAATTTTGGTTACATAGTGGCATTTAAGCCATTTGATGGAGAAGAATGGAAAAAAGTCAC
  AGTTACTAATCCTGATACTGGCCGATATGTCCATAAAGATGAAACCATGAGCCCTTCCACTGCATTTCAA
  GTTAAAGTCAAGGCCTTCAACAACAAAGGAGATGGACCTTACAGCCTAGTAGCAGTCATTAATTCAGCAC
  AAGACGCTCCCAGTGAAGCCCCAACAGAAGTAGGTGTAAAAGTCTTATCATCTTCTGAGATATCTGTTCA
  TTGGGAACATGTTTTAGAAAAAATAGTGGAAAGCTATCAGATTCGGTATTGGGCTGCCCATGACAAAGAA
  GAAGCTGCAAACAGAGTTCAAGTCACCAGCCAAGAGTACTCGGCCAGGCTCGAGAACCTTCTGCCAGACA
  CCCAGTATTTTATAGAAGTCGGGGCCTGCAATAGTGCAGGGTGTGGACCTCCAAGTGACATGATTGAGGC
  TTTCACCAAGAAAGCACCTCCTAGCCAGCCTCCAAGGATCATCAGTTCAGTAAGGTCTGGTTCACGCTAT
  ATAATCACCTGGGATCATGTCGTTGCACTATCAAATGAATCTACAGTGACGGGATATAAGGTACTCTACA
  GACCTGATGGCCAGCATGATGGCAAGCTGTATTCAACTCACAAACACTCCATAGAAGTCCCAATCCCCAG
  AGATGGAGAATACGTTGTGGAGGTTCGCGCGCACAGTGATGGAGGAGATGGAGTGGTGTCTCAAGTCAAA
  ATTTCAGGTGCACCCACCCTATCCCCAAGTCTTCTCGGCTTACTGCTGCCTGCCTTTGGCATCCTTGTCT
  ACTTGGAATTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220493 representing NM_175038
  Red=Cloning site Green=Tags(s)
  
  MKMWLLVSHLVIISITTCLAVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARASPFPVYKWRMNN
  GDVDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPPEERPEVRVKEG
  KGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYSCFVSSPSITKS
  VFSKFIPLIPIPERTTKPYPADIVVQFKDVYALMGQNVTLECFALGNPVPDIRWRKVLEPMPSTAEISTS
  GAVLKIFNIQLEDEGIYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYWPCVATGKPIPT
  IRWLKNGYAYHKGELRLYDVTFENAGMYQCIAENTYGAIYANAELKILALAPTFEMNPMKKKILAAKGGR
  VIIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNRGKANSTGTLVI
  TDPTRIILAPINADITVGENATMQCAASFDPALDLTFVWSFNGYVIDFNKENIHYQRNFMLDSNGELLIR
  NAQLKHAGRYTCTAQTIVDNSSASADLVVRGPPGPPGGLRIEDIRATSVALTWSRGSDNHSPISKYTIQT
  KTILSDDWKDAKTDPPIIEGNMEAARAVDLIPWMEYEFRVVATNTLGRGEPSIPSNRIKTDGAAPNVAPS
  DVGGGGGRNRELTITWAPLSREYHYGNNFGYIVAFKPFDGEEWKKVTVTNPDTGRYVHKDETMSPSTAFQ
  VKVKAFNNKGDGPYSLVAVINSAQDAPSEAPTEVGVKVLSSSEISVHWEHVLEKIVESYQIRYWAAHDKE
  EAANRVQVTSQEYSARLENLLPDTQYFIEVGACNSAGCGPPSDMIEAFTKKAPPSQPPRIISSVRSGSRY
  IITWDHVVALSNESTVTGYKVLYRPDGQHDGKLYSTHKHSIEVPIPRDGEYVVEVRAHSDGGDGVVSQVK
  ISGAPTLSPSLLGLLLPAFGILVYLEF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175038
• ORF Size: 3021 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175038.1, NP_778203.1
• RefSeq Size: 3394 bp
• RefSeq ORF: 3024 bp
• Locus ID: 1272
• Cytogenetics: 12q12
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: 'The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]'