IQSEC1 (NM_001134382) Human Tagged ORF Clone

CAT#: RG226333

IQSEC1 (GFP-tagged) - Human IQ motif and Sec7 domain 1 (IQSEC1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IQSEC1
• Synonyms: ARF-GEP100; ARFGEP100; BRAG2; GEP100
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226333 representing NM_001134382
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGCAGAAGACGCTATTTCGTCGAGGGCGAGGCCCCCAGCAGTGAGACTGGCACATCCCTGGACA
  GCCCCTCAGCCTACCCCCAGGGCCCCTTGGTGCCCGGTTCCAGCCTGAGCCCGGATCACTACGAGCACAC
  GTCAGTGGGAGCCTATGGGCTGTACTCGGGGCCGCCGGGGCAACAGCAGCGCACGCGGAGGCCCAAGCTG
  CAGCACTCGACCTCCATCCTGCGCAAGCAGGCTGAGGAGGAGGCCATCAAGCGCTCACGCTCACTCTCCG
  AGAGCTATGAGCTCTCCTCGGACCTGCAGGACAAGCAGGTGGAGATGCTAGAACGAAAGTATGGGGGGCG
  CCTGGTAACCCGCCATGCGGCCCGCACCATCCAGACGGCGTTTCGCCAGTACCAGATGAACAAGAACTTC
  GAGCGCTTGCGCAGCTCCATGTCAGAGAACCGCATGTCACGCCGGATTGTGCTGTCCAACATGAGGATGC
  AGTTCTCCTTTGAGGGGCCTGAGAAAGTGCACAGCTCCTACTTCGAGGGGAAGCAGGTCTCAGTGACTAA
  CGACGGCTCCCAGCTGGGAGCCCTGGTGTCCCCTGAGTGTGGTGACCTCAGCGAGCCCACCACCCTCAAG
  TCTCCGGCCCCCTCCAGTGACTTTGCGGACGCCATCACCGAGCTGGAGGACGCCTTCTCTAGGCAAGTGA
  AATCACTGGCCGAGTCCATCGACGATGCCCTCAACTGCCGCAGCCTGCACACTGAGGAGGCACCGGCCCT
  GGATGCGGCGCGGGCCCGGGACACCGAACCCCAGACAGCCCTGCACGGCATGGACCACCGCAAACTGGAC
  GAGATGACGGCCTCGTACAGTGATGTCACCCTGTACATCGATGAGGAGGAGCTGTCGCCCCCTCTGCCCC
  TCTCGCAGGCAGGGGACCGGCCGTCCAGCACCGAGTCGGACCTGCGGCTACGGGCTGGGGGCGCAGCCCC
  AGACTACTGGGCCCTGGCCCACAAAGAGGACAAGGCTGACACGGACACGAGCTGCCGGAGCACGCCGTCG
  CTGGAGCGGCAGGAGCAGCGGCTGCGGGTGGAGCATCTGCCGCTGCTCACCATCGAGCCACCCAGCGACA
  GCTCTGTGGACCTTAGTGACCGCTCGGAGCGGGGGTCACTCAAGAGGCAGAGTGCTTACGAGCGCAGCCT
  TGGCGGGCAGCAGGGCAGTCCCAAGCATGGTCCCCACAGCGGCGCCCCCAAGAGCCTCCCCCGGGAGGAG
  CCTGAGTTGCGGCCCCGGCCCCCCAGGCCCCTGGACAGCCACTTGGCCATCAATGGCTCAGCCAACCGGC
  AGAGCAAGTCTGAGTCGGACTACTCAGACGGTGACAATGACAGCATCAACAGCACGTCCAACTCCAACGA
  TACCATCAACTGCAGCTCCGAGTCATCGTCCCGTGACAGCCTGCGGGAGCAGACGCTCAGCAAGCAGACC
  TACCACAAGGAGGCCCGCAACAGCTGGGACTCGCCTGCCTTTAGCAACGATGTCATCCGCAAGAGGCACT
  ACCGCATCGGCCTGAACCTCTTCAACAAGAAGCCTGAGAAGGGAGTCCAGTACCTCATCGAGCGTGGCTT
  TGTGCCCGACACGCCCGTCGGGGTGGCCCACTTCCTGCTGCAGCGCAAGGGCCTCAGCCGGCAGATGATC
  GGCGAGTTCCTGGGCAACCGGCAGAAGCAGTTCAACCGTGACGTGCTCGACTGCGTCGTGGACGAGATGG
  ACTTCTCTACCATGGAGCTGGATGAGGCCCTCAGGAAATTCCAGGCGCACATCCGTGTCCAAGGGGAGGC
  TCAGAAAGTGGAGCGGCTCATAGAGGCGTTCAGCCAGCGCTACTGCATCTGCAACCCTGGGGTGGTGCGG
  CAATTCCGGAACCCAGACACCATTTTCATCCTGGCCTTCGCCATCATCCTGCTGAACACCGACATGTACA
  GCCCCAATGTCAAGCCCGAGCGGAAAATGAAGCTAGAGGACTTCATCAAGAACCTCCGAGGTGTGGACGA
  TGGTGAGGACATTCCCCGTGAGATGCTGATGGGGATCTATGAACGGATCCGTAAGCGAGAGCTAAAGACC
  AATGAGGACCATGTGTCCCAGGTGCAGAAGGTGGAGAAGCTCATTGTGGGGAAAAAGCCGATCGGATCCC
  TGCATCCCGGGCTCGGCTGTGTGCTCTCTCTGCCCCACCGTCGGTTGGTCTGCTACTGCCGGCTCTTTGA
  GGTTCCAGACCCAAACAAGCCCCAGAAACTCGGACTACACCAGCGAGAAATCTTCCTGTTCAACGACCTC
  CTGGTGGTCACCAAGATCTTCCAGAAGAAGAAGAACTCGGTGACGTACAGCTTCCGACAGTCCTTCTCCT
  TGTACGGCATGCAGGTCCTGCTCTTCGAGAACCAGTACTACCCCAATGGCATCCGGCTCACCTCGTCTGT
  CCCCGGAGCAGATATCAAAGTGTTAATAAACTTCAACGCCCCCAACCCTCAAGACCGGAAGAAATTCACC
  GATGACCTGCGGGAGTCCATTGCGGAAGTCCAAGAGATGGAGAAGCACAGGATAGAGTCGGAGCTCGAGA
  AGCAGAAAGGCGTCGTGCGGCCCAGCATGTCCCAGTGCTCTAGCCTCAAAAAGGAGTCGGGCAACGGAAC
  ACTGAGCCGGGCCTGCCTGGACGACAGCTATGCCAGCGGTGAGGGCCTCAAGCGCAGCGCCCTCAGCAGC
  TCCCTGCGGGACCTCTCGGAAGCCGGGAAGCGAGGGCGTCGCAGCAGTGCGGGATCGCTAGAGAGCAATG
  TGGAAGGGTCCATCATTAGCAGTCCTCACATGCGCCGGAGAGCTACATCAACACGAGAGTGTCCATCTCG
  CCCACACCAGACTATGCCCAACTCATCTTCCCTCCTGGGCTCCTTATTCGGGAGCAAGAGAGGGAAGCCC
  CCTCCCCAGGCCCACCTGCCCTCAGCCCCAGCCCTGCCACCCCCCCACCCACCGGTGGTCCTGCCTCACT
  TGCAGCACTCTGTGGCTGGCCACCACCTGGGGCCCCCAGAGGGGCTGCCGCAGGCCGCCATGCACGGGCA
  TCACACCCAGTACTGCCACATGCAGAACCCTCCCCCGTACCACCATCACCACCACCACCACCCACCCCAG
  CACATCCAGCACGCACACCAGTACCACCACGGCCCCCATGGGGGCCACCCAGCCTACGGGGCCCATGCCC
  ACGGCCACCCGCCGCTGCCCTCGGCCCACGTGGGGCACACAGTGCACCACCATGGGCAGCCCCCTGCCCC
  GCCGCCCCCCACCAGCAGCAAGGCCAAACCCAGCGGCATCAGCACAATTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226333 representing NM_001134382
  Red=Cloning site Green=Tags(s)
  
  MACRRRYFVEGEAPSSETGTSLDSPSAYPQGPLVPGSSLSPDHYEHTSVGAYGLYSGPPGQQQRTRRPKL
  QHSTSILRKQAEEEAIKRSRSLSESYELSSDLQDKQVEMLERKYGGRLVTRHAARTIQTAFRQYQMNKNF
  ERLRSSMSENRMSRRIVLSNMRMQFSFEGPEKVHSSYFEGKQVSVTNDGSQLGALVSPECGDLSEPTTLK
  SPAPSSDFADAITELEDAFSRQVKSLAESIDDALNCRSLHTEEAPALDAARARDTEPQTALHGMDHRKLD
  EMTASYSDVTLYIDEEELSPPLPLSQAGDRPSSTESDLRLRAGGAAPDYWALAHKEDKADTDTSCRSTPS
  LERQEQRLRVEHLPLLTIEPPSDSSVDLSDRSERGSLKRQSAYERSLGGQQGSPKHGPHSGAPKSLPREE
  PELRPRPPRPLDSHLAINGSANRQSKSESDYSDGDNDSINSTSNSNDTINCSSESSSRDSLREQTLSKQT
  YHKEARNSWDSPAFSNDVIRKRHYRIGLNLFNKKPEKGVQYLIERGFVPDTPVGVAHFLLQRKGLSRQMI
  GEFLGNRQKQFNRDVLDCVVDEMDFSTMELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCICNPGVVR
  QFRNPDTIFILAFAIILLNTDMYSPNVKPERKMKLEDFIKNLRGVDDGEDIPREMLMGIYERIRKRELKT
  NEDHVSQVQKVEKLIVGKKPIGSLHPGLGCVLSLPHRRLVCYCRLFEVPDPNKPQKLGLHQREIFLFNDL
  LVVTKIFQKKKNSVTYSFRQSFSLYGMQVLLFENQYYPNGIRLTSSVPGADIKVLINFNAPNPQDRKKFT
  DDLRESIAEVQEMEKHRIESELEKQKGVVRPSMSQCSSLKKESGNGTLSRACLDDSYASGEGLKRSALSS
  SLRDLSEAGKRGRRSSAGSLESNVEGSIISSPHMRRRATSTRECPSRPHQTMPNSSSLLGSLFGSKRGKP
  PPQAHLPSAPALPPPHPPVVLPHLQHSVAGHHLGPPEGLPQAAMHGHHTQYCHMQNPPPYHHHHHHHPPQ
  HIQHAHQYHHGPHGGHPAYGAHAHGHPPLPSAHVGHTVHHHGQPPAPPPPTSSKAKPSGISTIV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134382
• ORF Size: 3342 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001134382.1, NP_001127854.1
• RefSeq Size: 7229
• RefSeq ORF: 3345
• Locus ID: 9922
• Protein Pathways: Endocytosis