DOC2A (NM_001282063) Human Tagged ORF Clone

CAT#: RG237985

DOC2A (GFP-tagged) - Human double C2-like domains, alpha (DOC2A), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: DOC2A
• Synonyms: Doc2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237985 representing NM_001282063.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTA
  CCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCC
  ATGAGGGGCCGCAGGGGCGATCGCATGACCATCAACATCCAGGAGCACATGGCCATCAACGTGTGCCCC
  GGGCCCATCCGGCCCATCCGCCAGATCTCTGACTACTTCCCCCGGGGACCAGGACCTGAAGGGGGCGGC
  GGGGGCGGCGGGGAGGCCCCCGCCCATCTGGTCCCCCTGGCTCTGGCCCCCCCTGCAGCCCTCCTTGGG
  GCCACCACGCCTGAGGATGGTGCGGAGGTGGACAGCTATGACTCGGATGATGCCACCGCCCTAGGCACG
  CTGGAGTTTGACCTTCTCTACGACCGGGCCTCCTGCACTCTGCACTGTAGCATCCTCAGGGCCAAGGGC
  CTCAAGCCCATGGATTTCAATGGCCTCGCCGACCCCTACGTCAAGCTGCACTTGCTGCCTGGAGCCTGT
  AAGGCCAATAAGCTAAAAACGAAGACTCAGAGGAACACACTGAATCCCGTGTGGAATGAGGACCTGACT
  TACAGCGGGATCACAGATGACGACATCACGCACAAGGTGCTCAGGATCGCCGTCTGTGATGAGGACAAG
  CTGAGTCACAATGAGTTTATTGGGGAGATCCGCGTGCCCCTCCGCCGCCTCAAGCCTTCGCAGAAGAAG
  CATTTTAACATCTGCCTCGAGCGCCAGGTCCCGCTGGCGTCCCCCTCTTCCATGTCAGCGGCGCTGAGG
  GGCATCTCCTGTTATCTGAAGGAGTTGGAGCAGGCGGAGCAGGGGCAGGGGCTGCTGGAGGAGCGTGGC
  CGCATCCTGCTGAGTCTCAGCTACAGCTCGCGGCGCCGGGGACTGCTGGTAGGCATCTTGCGCTGCGCC
  CATCTGGCTGCCATGGACGTCAACGGTTACTCGGACCCCTACGTCAAGACGTACCTGAGGCCCGATGTG
  GACAAGAAATCCAAGCATAAGACGTGTGTGAAGAAGAAGACTCTCAACCCAGAATTTAACGAGGAGTTT
  TTCTACGAGATAGAGCTCTCCACTCTGGCCACCAAGACCCTGGAAGTCACCGTCTGGGACTATGACATT
  GGCAAATCCAATGACTTCATTGGTGGCGTGTCCCTGGGGCCAGGTGCCCGAGGCGAGGCTCGGAAGCAC
  TGGAGTGACTGCCTGCAGCAGCCGGACGCAGCCCTGGAGCGCTGGCACACCCTGACCAGTGAGCTGCCC
  CCTGCGGCCGGGGCTCTGTCCTCAGCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237985
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRGRRGDRMTINIQEHMAINVCPGPIRPIRQISDYFPRGPGPEGGGGGGGEAPAHLVPLALAPPAALLG
  ATTPEDGAEVDSYDSDDATALGTLEFDLLYDRASCTLHCSILRAKGLKPMDFNGLADPYVKLHLLPGAC
  KANKLKTKTQRNTLNPVWNEDLTYSGITDDDITHKVLRIAVCDEDKLSHNEFIGEIRVPLRRLKPSQKK
  HFNICLERQVPLASPSSMSAALRGISCYLKELEQAEQGQGLLEERGRILLSLSYSSRRRGLLVGILRCA
  HLAAMDVNGYSDPYVKTYLRPDVDKKSKHKTCVKKKTLNPEFNEEFFYEIELSTLATKTLEVTVWDYDI
  GKSNDFIGGVSLGPGARGEARKHWSDCLQQPDAALERWHTLTSELPPAAGALSSA
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282063
• ORF Size: 1200 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282063.1, NP_001268992.1
• RefSeq Size: 2090 bp
• RefSeq ORF: 1203 bp
• Locus ID: 8448
• Cytogenetics: 16p11.2
• Protein Families: Secreted Protein
• MW: 44 kDa
• Gene Summary: There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]