Oprk1 (NM_017167) Rat Untagged Clone

CAT#: RN200637

Oprk1 (untagged ORF) - Rat opioid receptor, kappa 1 (Oprk1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Oprk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200637 representing NM_017167
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCCCCATCCAGATTTTCCGCGGAGAGCCAGGCCCTACCTGTGCTCCCAGTGCTTGCCTACTCC
  CCAACAGCAGCTCTTGGTTCCCCAACTGGGCCGAATCGGACAGCAATGGCAGTGTGGGCTCCGAGGACCA
  GCAGCTGGAGCCCGCGCACATCTCTCCAGCCATCCCTGTTATCATCACCGCTGTCTACTCTGTGGTGTTT
  GTGGTGGGCTTAGTGGGCAATTCCCTGGTCATGTTTGTCATCATCCGATACACAAAGATGAAGACCGCAA
  CCAACATCTACATATTTAACCTGGCTTTGGCAGATGCTTTGGTTACTACCACTATGCCCTTCCAGAGTGC
  TGTCTACTTGATGAATTCTTGGCCTTTTGGAGATGTTCTGTGCAAGATTGTCATTTCCATTGACTACTAC
  AACATGTTTACCAGCATATTCACCTTGACCATGATGAGTGTGGACCGCTACATTGCCGTGTGCCACCCTG
  TGAAAGCTTTGGATTTCCGAACACCTTTGAAAGCAAAGATCATCAACATCTGCATTTGGCTACTGGCATC
  ATCTGTTGGTATATCAGCGATAGTCCTTGGAGGCACCAAAGTCAGGGAAGATGTGGATGTCATTGAATGC
  TCCTTGCAGTTTCCTGATGATGAATATTCCTGGTGGGACCTCTTCATGAAGATCTGTGTCTTCGTCTTTG
  CCTTTGTTATCCCTGTCTTAATCATCATTGTCTGCTACACCCTGATGATCCTGCGCTTGAAGAGTGTCCG
  GCTCCTCTCGGGCTCTCGAGAGAAGGACCGAAATCTCCGCCGGATCACCAAGCTGGTGCTGGTAGTGGTT
  GCAGTCTTCATCATCTGTTGGACCCCCATCCACATCTTTATCCTGGTCGAGGCTCTAGGCAGCACCTCCC
  ACAGCACAGCTGTCCTCTCTAGCTATTACTTCTGCATTGCCTTGGGTTATACCAACAGCAGCTTGAATCC
  TGTTCTCTATGCCTTTCTTGATGAAAACTTCAAGCGGTGTTTTAGGGACTTCTGCTTCCCCATTAAGATG
  CGAATGGAGCGCCAGAGCACAAACAGAGTTAGAAACACAGTTCAGGATCCTGCTTCCATGAGGGATGTGG
  GTGGGATGAATAAGCCAGTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_017167
• ORF Size: 1143 bp
• Insert Size: 1143
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_017167.2, NP_058863.1
• RefSeq Size: 1358
• RefSeq ORF: 1143
• Locus ID: 29335
• Gene Summary: This gene encodes an opioid receptor, which is a member of the 7 transmembrane-spanning G protein-coupled receptor family. It functions as a receptor for endogenous ligands, as well as a receptor for various synthetic opioids. Ligand binding results in inhibition of adenylate cyclase activity and neurotransmitter release. This opioid receptor plays a role in the perception of pain and mediating the hypolocomotor, analgesic and aversive actions of synthetic opioids. Variations in this gene have also been associated with alcohol dependence and opiate addiction. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017]
  Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.