Mdh1 (NM_033235) Rat Untagged Clone

CAT#: RN210984

Mdh1 (untagged ORF) - Rat malate dehydrogenase 1, NAD (soluble) (Mdh1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Mdh1
• Synonyms: Mdhl; MDL1; Mor2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210984 representing NM_033235
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGCCAATCAGAGTCCTCGTGACAGGAGCAGCCGGTCAGATTGCATATTCGCTGCTGTACAGCA
  TTGGAAATGGATCTGTCTTTGGGAAAGACCAGCCCATCATTCTTGTGCTGTTGGACATCACCCCCATGAT
  GGGTGTTCTGGACGGTGTCCTGATGGAGCTGCAAGACTGTGCCCTTCCCCTTCTGCAGGATGTCATTGCA
  ACAGACAAAGAAGAGGTTGCCTTCAAAGACCTGGACGTGGCTGTCCTTGTGGGCTCCATGCCAAGAAGGG
  AGGGCATGGAGAGGAAGGACCTACTGAAAGCCAACGTGAAGATCTTCAAATCCCAGGGCGCAGCCTTGGA
  GAAGTACGCCAAGAAATCAGTTAAGGTCATTGTTGTGGGGAACCCAGCCAATACAAACTGCCTGACGGCC
  TCCAAGTCAGCACCATCGATCCCCAAGGAGAACTTCAGTTGCCTGACTCGATTGGACCACAACCGAGCAA
  AATCTCAAATTGCTCTTAAACTCGGTGTAACCGCTGATGATGTAAAAAATGTCATTATCTGGGGAAATCA
  TTCATCAACCCAGTATCCAGATGTCAATCATGCCAAGGTGAAATTGCAAGGAAAAGAAGTTGGTGTGTAT
  GAAGCCCTCAAAGACGACAGCTGGCTCAAGGGAGAGTTCATCACGACTGTGCAGCAGCGTGGTGCTGCTG
  TCATCAAGGCTCGGAAGCTGTCCAGTGCCATGTCTGCTGCGAAGGCCATCTCGGACCACATCAGAGACAT
  CTGGTTTGGAACCCCGGAGGGCGAGTTCGTGTCGATGGGCGTAATCTCTGATGGCAACTCCTATGGTGTC
  CCTGATGACCTGCTCTACTCGTTCCCTGTCGTGATCAAGAATAAGACCTGGAAGTTTGTTGAAGGCCTCC
  CCATTAACGACTTCTCCCGTGAGAAGATGGACCTGACAGCAAAGGAGCTGACCGAGGAAAAGGAAACGGC
  TTTTGAGTTTCTCTCCTCCGCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_033235
• ORF Size: 1005 bp
• Insert Size: 1005
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_033235.2, NP_150238.1
• RefSeq Size: 1878
• RefSeq ORF: 1005
• Locus ID: 24551
• Gene Summary: This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. [provided by RefSeq, Feb 2016]
  Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Mdh1) results from translation termination at the upstream UGA stop codon, while the longer isoform (Mdh1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (Mdh1), which is localized in the cytosol.